Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficit
Context: Sanchi promotes wound healing by repressing fibroblast proliferation. Objective: This study examined the effect of Sanchi on keratinocytes (KCs) and microvascular endothelial cells (MECs) and rats with skin injury. Materials & methods: Hydrogels containing different concentrations o...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024030135 |
| _version_ | 1827316490846601216 |
|---|---|
| author | Xiaoling Li Zhiwei Zhao Bo Cui Yanfeng Li |
| author_facet | Xiaoling Li Zhiwei Zhao Bo Cui Yanfeng Li |
| author_sort | Xiaoling Li |
| collection | DOAJ |
| description | Context: Sanchi promotes wound healing by repressing fibroblast proliferation. Objective: This study examined the effect of Sanchi on keratinocytes (KCs) and microvascular endothelial cells (MECs) and rats with skin injury. Materials & methods: Hydrogels containing different concentrations of Sanchi extract were prepared to observe wound closure over 10 days. SD rats were divided into the control, Hydrogel, 5% Hydrogel, 10% Hydrogel, 10% Hydrogel + Ad5-NC, and 10% Hydrogel + Ad5-IL1B groups. KCs and MECs were induced with H2O2 for 24 h. Cell viability, apoptosis, and the levels of inflammation- and oxidative stress-related factors were examined. The effect of IL1B on wound healing was also evaluated. Results: Compared to the Control group (83% ± 7.4%) or Hydrogel without Sanchi extract (84% ± 8.5%), Hydrogel with 5% (95% closure ± 4.0%) or 10% Sanchi extract (98% ± 1.7%) accelerated wound healing in rats and attenuated inflammation and oxidative stress. Hydrogels containing Sanchi extract increased collagen deposition and CD31 expression in tissues. H2O2 (100 μM) induced injury in KCs and MECs, whereas Sanchi rescued the viability of KCs and MECs. Sanchi inhibited cell inflammation and oxidative stress and decreased apoptosis. As Sanchi blocked the NFκB pathway via IL1B, IL1B mitigated the therapeutic effect of Sanchi. Discussion and conclusion: Sanchi demonstrated therapeutic effects on wound healing in rats by promoting KCs and MECs activity. These findings provide valuable information for the clinical application of Sanchi, which needs to be validated in future clinical trials. |
| first_indexed | 2024-03-07T14:28:38Z |
| format | Article |
| id | doaj.art-c6666bffd75947cdb8d09ce2c94fc3aa |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:15:22Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c6666bffd75947cdb8d09ce2c94fc3aa2024-03-17T07:56:58ZengElsevierHeliyon2405-84402024-03-01105e26982Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficitXiaoling Li0Zhiwei Zhao1Bo Cui2Yanfeng Li3Health Control Department, Luoyang Orthopedic Hospital of Henan Province (Orthopedic Hospital of Henan Province), Zhengzhou, 450016, Henan, PR ChinaDepartment of Hand Surgery & Micro Orthopedics, Luoyang Orthopedic Hospital of Henan Province (Orthopedic Hospital of Henan Province), Zhengzhou, 450016, Henan, PR China; Corresponding authorDepartment of Hand Surgery & Micro Orthopedics, Luoyang Orthopedic Hospital of Henan Province (Orthopedic Hospital of Henan Province), No. 100, Yongping Road, Guancheng Hui District, Zhengzhou 450016, Henan, PR China.Department of Hand Surgery & Micro Orthopedics, Luoyang Orthopedic Hospital of Henan Province (Orthopedic Hospital of Henan Province), Zhengzhou, 450016, Henan, PR ChinaDepartment of Hand Surgery & Micro Orthopedics, Luoyang Orthopedic Hospital of Henan Province (Orthopedic Hospital of Henan Province), Zhengzhou, 450016, Henan, PR ChinaContext: Sanchi promotes wound healing by repressing fibroblast proliferation. Objective: This study examined the effect of Sanchi on keratinocytes (KCs) and microvascular endothelial cells (MECs) and rats with skin injury. Materials & methods: Hydrogels containing different concentrations of Sanchi extract were prepared to observe wound closure over 10 days. SD rats were divided into the control, Hydrogel, 5% Hydrogel, 10% Hydrogel, 10% Hydrogel + Ad5-NC, and 10% Hydrogel + Ad5-IL1B groups. KCs and MECs were induced with H2O2 for 24 h. Cell viability, apoptosis, and the levels of inflammation- and oxidative stress-related factors were examined. The effect of IL1B on wound healing was also evaluated. Results: Compared to the Control group (83% ± 7.4%) or Hydrogel without Sanchi extract (84% ± 8.5%), Hydrogel with 5% (95% closure ± 4.0%) or 10% Sanchi extract (98% ± 1.7%) accelerated wound healing in rats and attenuated inflammation and oxidative stress. Hydrogels containing Sanchi extract increased collagen deposition and CD31 expression in tissues. H2O2 (100 μM) induced injury in KCs and MECs, whereas Sanchi rescued the viability of KCs and MECs. Sanchi inhibited cell inflammation and oxidative stress and decreased apoptosis. As Sanchi blocked the NFκB pathway via IL1B, IL1B mitigated the therapeutic effect of Sanchi. Discussion and conclusion: Sanchi demonstrated therapeutic effects on wound healing in rats by promoting KCs and MECs activity. These findings provide valuable information for the clinical application of Sanchi, which needs to be validated in future clinical trials.http://www.sciencedirect.com/science/article/pii/S2405844024030135KeratinocytesMicrovascular endothelial cellsTraditional Chinese medicineHydrogels |
| spellingShingle | Xiaoling Li Zhiwei Zhao Bo Cui Yanfeng Li Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficit Heliyon Keratinocytes Microvascular endothelial cells Traditional Chinese medicine Hydrogels |
| title | Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficit |
| title_full | Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficit |
| title_fullStr | Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficit |
| title_full_unstemmed | Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficit |
| title_short | Sanchi-mediated inactivation of IL1B accelerates wound healing through the NFκB pathway deficit |
| title_sort | sanchi mediated inactivation of il1b accelerates wound healing through the nfκb pathway deficit |
| topic | Keratinocytes Microvascular endothelial cells Traditional Chinese medicine Hydrogels |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024030135 |
| work_keys_str_mv | AT xiaolingli sanchimediatedinactivationofil1baccelerateswoundhealingthroughthenfkbpathwaydeficit AT zhiweizhao sanchimediatedinactivationofil1baccelerateswoundhealingthroughthenfkbpathwaydeficit AT bocui sanchimediatedinactivationofil1baccelerateswoundhealingthroughthenfkbpathwaydeficit AT yanfengli sanchimediatedinactivationofil1baccelerateswoundhealingthroughthenfkbpathwaydeficit |