Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells
Skeletal muscle possesses a robust capacity to regenerate functional architectures with a unidirectional orientation. In this study, we successfully arranged skeletal myoblast (C2C12) cells along micropatterned gold strips on which chitohexaose was deposited via a vectorial chain immobilization appr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-01-01
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| Series: | Biomolecules |
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| Online Access: | http://www.mdpi.com/2218-273X/6/1/12 |
| _version_ | 1828826123778129920 |
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| author | Pornthida Poosala Takuya Kitaoka |
| author_facet | Pornthida Poosala Takuya Kitaoka |
| author_sort | Pornthida Poosala |
| collection | DOAJ |
| description | Skeletal muscle possesses a robust capacity to regenerate functional architectures with a unidirectional orientation. In this study, we successfully arranged skeletal myoblast (C2C12) cells along micropatterned gold strips on which chitohexaose was deposited via a vectorial chain immobilization approach. Hexa-N-acetyl-d-glucosamine (GlcNAc6) was site-selectively modified at its reducing end with thiosemicarbazide, then immobilized on a gold substrate in striped micropatterns via S–Au chemisorption. Gold micropatterns ranged from 100 to 1000 µm in width. Effects of patterning geometries on C2C12 cell alignment, morphology, and gene expression were investigated. Unidirectional alignment of C2C12 cells having GlcNAc6 receptors was clearly observed along the micropatterns. Decreasing striped pattern width increased cell attachment and proliferation, suggesting that the fixed GlcNAc6 and micropatterns impacted cell function. Possibly, interactions between nonreducing end groups of fixed GlcNAc6 and cell surface receptors initiated cellular alignment. Our technique for mimicking native tissue organization should advance applications in tissue engineering. |
| first_indexed | 2024-12-12T14:32:12Z |
| format | Article |
| id | doaj.art-c66a00b358ec4bb094dfb5fa0672e416 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-12T14:32:12Z |
| publishDate | 2016-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c66a00b358ec4bb094dfb5fa0672e4162022-12-22T00:21:29ZengMDPI AGBiomolecules2218-273X2016-01-01611210.3390/biom6010012biom6010012Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast CellsPornthida Poosala0Takuya Kitaoka1Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, JapanFaculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, JapanSkeletal muscle possesses a robust capacity to regenerate functional architectures with a unidirectional orientation. In this study, we successfully arranged skeletal myoblast (C2C12) cells along micropatterned gold strips on which chitohexaose was deposited via a vectorial chain immobilization approach. Hexa-N-acetyl-d-glucosamine (GlcNAc6) was site-selectively modified at its reducing end with thiosemicarbazide, then immobilized on a gold substrate in striped micropatterns via S–Au chemisorption. Gold micropatterns ranged from 100 to 1000 µm in width. Effects of patterning geometries on C2C12 cell alignment, morphology, and gene expression were investigated. Unidirectional alignment of C2C12 cells having GlcNAc6 receptors was clearly observed along the micropatterns. Decreasing striped pattern width increased cell attachment and proliferation, suggesting that the fixed GlcNAc6 and micropatterns impacted cell function. Possibly, interactions between nonreducing end groups of fixed GlcNAc6 and cell surface receptors initiated cellular alignment. Our technique for mimicking native tissue organization should advance applications in tissue engineering.http://www.mdpi.com/2218-273X/6/1/12biointerfacecarbohydrate-protein interactioncell alignmentmicropatternmouse myoblast celloligosaccharidetissue engineering |
| spellingShingle | Pornthida Poosala Takuya Kitaoka Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells Biomolecules biointerface carbohydrate-protein interaction cell alignment micropattern mouse myoblast cell oligosaccharide tissue engineering |
| title | Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells |
| title_full | Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells |
| title_fullStr | Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells |
| title_full_unstemmed | Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells |
| title_short | Chitooligomer-Immobilized Biointerfaces with Micropatterned Geometries for Unidirectional Alignment of Myoblast Cells |
| title_sort | chitooligomer immobilized biointerfaces with micropatterned geometries for unidirectional alignment of myoblast cells |
| topic | biointerface carbohydrate-protein interaction cell alignment micropattern mouse myoblast cell oligosaccharide tissue engineering |
| url | http://www.mdpi.com/2218-273X/6/1/12 |
| work_keys_str_mv | AT pornthidapoosala chitooligomerimmobilizedbiointerfaceswithmicropatternedgeometriesforunidirectionalalignmentofmyoblastcells AT takuyakitaoka chitooligomerimmobilizedbiointerfaceswithmicropatternedgeometriesforunidirectionalalignmentofmyoblastcells |