Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer
Simultaneous detection of multiple analytes from a single biological sample is gaining more attention in the development of more reliable and point-of-care diagnostic devices. We developed a multiplexed strategy that combined outcomes of clinical biomarkers with analysis of the protein corona that f...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/13/1/93 |
| _version_ | 1797543042449670144 |
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| author | Damiano Caputo Luca Digiacomo Chiara Cascone Daniela Pozzi Sara Palchetti Riccardo Di Santo Erica Quagliarini Roberto Coppola Morteza Mahmoudi Giulio Caracciolo |
| author_facet | Damiano Caputo Luca Digiacomo Chiara Cascone Daniela Pozzi Sara Palchetti Riccardo Di Santo Erica Quagliarini Roberto Coppola Morteza Mahmoudi Giulio Caracciolo |
| author_sort | Damiano Caputo |
| collection | DOAJ |
| description | Simultaneous detection of multiple analytes from a single biological sample is gaining more attention in the development of more reliable and point-of-care diagnostic devices. We developed a multiplexed strategy that combined outcomes of clinical biomarkers with analysis of the protein corona that forms around graphene oxide sheets upon exposure to patient’s plasma. As a paradigmatic case study, we selected pancreatic ductal adenocarcinoma (PDAC), mainly because of the absence of effective detection strategies that resulted in an extremely low five-year survival rate after diagnosis (<10%). Association of protein corona analysis and haemoglobin levels discriminated PDAC patients from healthy volunteers in up to 90% of cases. If further confirmed in larger-cohort studies, this approach may be used in the detection of PDAC. |
| first_indexed | 2024-03-10T13:40:03Z |
| format | Article |
| id | doaj.art-c66a19887c1046f8bfef744d034f45c0 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T13:40:03Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-c66a19887c1046f8bfef744d034f45c02023-11-21T03:10:52ZengMDPI AGCancers2072-66942020-12-011319310.3390/cancers13010093Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic CancerDamiano Caputo0Luca Digiacomo1Chiara Cascone2Daniela Pozzi3Sara Palchetti4Riccardo Di Santo5Erica Quagliarini6Roberto Coppola7Morteza Mahmoudi8Giulio Caracciolo9Department of Surgery, University Campus Bio-Medico di Roma, Via Alvaro del Portillo 200, 00128 Rome, ItalyDepartment of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, ItalyDepartment of Surgery, University Campus Bio-Medico di Roma, Via Alvaro del Portillo 200, 00128 Rome, ItalyDepartment of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, ItalyDepartment of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, ItalyDepartment of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, ItalyDepartment of Surgery, University Campus Bio-Medico di Roma, Via Alvaro del Portillo 200, 00128 Rome, ItalyDepartment of Radiology and Precision Health Program, Michigan State University, East Lansing, MI 48824, USADepartment of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, ItalySimultaneous detection of multiple analytes from a single biological sample is gaining more attention in the development of more reliable and point-of-care diagnostic devices. We developed a multiplexed strategy that combined outcomes of clinical biomarkers with analysis of the protein corona that forms around graphene oxide sheets upon exposure to patient’s plasma. As a paradigmatic case study, we selected pancreatic ductal adenocarcinoma (PDAC), mainly because of the absence of effective detection strategies that resulted in an extremely low five-year survival rate after diagnosis (<10%). Association of protein corona analysis and haemoglobin levels discriminated PDAC patients from healthy volunteers in up to 90% of cases. If further confirmed in larger-cohort studies, this approach may be used in the detection of PDAC.https://www.mdpi.com/2072-6694/13/1/93pancreatic cancergraphene oxideearly detection |
| spellingShingle | Damiano Caputo Luca Digiacomo Chiara Cascone Daniela Pozzi Sara Palchetti Riccardo Di Santo Erica Quagliarini Roberto Coppola Morteza Mahmoudi Giulio Caracciolo Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer Cancers pancreatic cancer graphene oxide early detection |
| title | Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer |
| title_full | Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer |
| title_fullStr | Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer |
| title_full_unstemmed | Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer |
| title_short | Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer |
| title_sort | synergistic analysis of protein corona and haemoglobin levels detects pancreatic cancer |
| topic | pancreatic cancer graphene oxide early detection |
| url | https://www.mdpi.com/2072-6694/13/1/93 |
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