Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins
The last two decades have seen an increasing demand for new protein-modification methods from the biotech industry and biomedical research communities. Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. In this regard, t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/458 |
| _version_ | 1797498760831434752 |
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| author | Dingpeng Zhang Zhen Wang Side Hu Julien Lescar James P. Tam Chuan-Fa Liu |
| author_facet | Dingpeng Zhang Zhen Wang Side Hu Julien Lescar James P. Tam Chuan-Fa Liu |
| author_sort | Dingpeng Zhang |
| collection | DOAJ |
| description | The last two decades have seen an increasing demand for new protein-modification methods from the biotech industry and biomedical research communities. Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. In this regard, the recently discovered peptidyl Asx-specific ligases (PALs) have emerged as powerful biotechnological tools in recent years. However, as a new class of peptide ligases, their scope and application remain underexplored. Herein, we report the use of a new PAL, VyPAL2, for a diverse range of protein modifications. We successfully showed that VyPAL2 was an efficient biocatalyst for protein labelling, inter-protein ligation, and protein cyclization. The labelled or cyclized protein ligands remained functionally active in binding to their target receptors. We also demonstrated on-cell labelling of protein ligands pre-bound to cellular receptors and cell-surface engineering via modifying a covalently anchored peptide substrate pre-installed on cell-surface glycans. Together, these examples firmly establish Asx-specific ligases, such as VyPAL2, as the biocatalysts of the future for site-specific protein modification, with a myriad of applications in basic research and drug discovery. |
| first_indexed | 2024-03-10T03:37:59Z |
| format | Article |
| id | doaj.art-c66b8278e3da4d929044afb422f1816e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:37:59Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c66b8278e3da4d929044afb422f1816e2023-11-23T11:40:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123145810.3390/ijms23010458Vypal2: A Versatile Peptide Ligase for Precision Tailoring of ProteinsDingpeng Zhang0Zhen Wang1Side Hu2Julien Lescar3James P. Tam4Chuan-Fa Liu5School of Biological Sciences, Nanyang Technological University, Singapore 637551, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore 637551, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore 637551, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore 637551, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore 637551, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore 637551, SingaporeThe last two decades have seen an increasing demand for new protein-modification methods from the biotech industry and biomedical research communities. Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. In this regard, the recently discovered peptidyl Asx-specific ligases (PALs) have emerged as powerful biotechnological tools in recent years. However, as a new class of peptide ligases, their scope and application remain underexplored. Herein, we report the use of a new PAL, VyPAL2, for a diverse range of protein modifications. We successfully showed that VyPAL2 was an efficient biocatalyst for protein labelling, inter-protein ligation, and protein cyclization. The labelled or cyclized protein ligands remained functionally active in binding to their target receptors. We also demonstrated on-cell labelling of protein ligands pre-bound to cellular receptors and cell-surface engineering via modifying a covalently anchored peptide substrate pre-installed on cell-surface glycans. Together, these examples firmly establish Asx-specific ligases, such as VyPAL2, as the biocatalysts of the future for site-specific protein modification, with a myriad of applications in basic research and drug discovery.https://www.mdpi.com/1422-0067/23/1/458biocatalystsVyPAL2protein labelingprotein cyclizationcell surface labeling |
| spellingShingle | Dingpeng Zhang Zhen Wang Side Hu Julien Lescar James P. Tam Chuan-Fa Liu Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins International Journal of Molecular Sciences biocatalysts VyPAL2 protein labeling protein cyclization cell surface labeling |
| title | Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins |
| title_full | Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins |
| title_fullStr | Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins |
| title_full_unstemmed | Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins |
| title_short | Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins |
| title_sort | vypal2 a versatile peptide ligase for precision tailoring of proteins |
| topic | biocatalysts VyPAL2 protein labeling protein cyclization cell surface labeling |
| url | https://www.mdpi.com/1422-0067/23/1/458 |
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