A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme
Abstract Background Chinese herbs have been widely used for thousands of years. In order to kill or control insects and fungus during the cultivation of herb plants, pesticides have played a significant role. More than 30 kinds of pesticides have been documented in the latest version of Chinese Phar...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-03-01
|
| Series: | Chinese Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13020-020-00304-2 |
| _version_ | 1818058386665635840 |
|---|---|
| author | Jinchao Wei Yan Xue Jiayi Dong Shuangpeng Wang Hao Hu Hao Gao Peng Li Yitao Wang |
| author_facet | Jinchao Wei Yan Xue Jiayi Dong Shuangpeng Wang Hao Hu Hao Gao Peng Li Yitao Wang |
| author_sort | Jinchao Wei |
| collection | DOAJ |
| description | Abstract Background Chinese herbs have been widely used for thousands of years. In order to kill or control insects and fungus during the cultivation of herb plants, pesticides have played a significant role. More than 30 kinds of pesticides have been documented in the latest version of Chinese Pharmacopoeia. It is urgent to develop new analytical methods for pesticide detection. Methods A fluorescent detection system was established by using Cerium based fluorescent polymer and Sm-CeO2. As a new doped nanozyme, Sm-CeO2 exhibits OPH-like activity to hydrolyze OPP pesticide. Results The morphology of the prepared CFP and Sm-CeO2 were characterized. The optimal conditions for CFP synthesis are CeCl3 (16 mmol L−1, 200 μL), ATP (4 mmol L−1, 200 μL) and Tris buffer (5 mmol L−1, 500 μL, pH 8.0). Sm-CeO2 shows the best mimic activity to hydrolyze OPP pesticide at pH = 8.0. The results exhibit good linear relationship between fluorescent quenching effect and MP in the range of 2–50 μmol L−1. Finally, this fluorescent technique was successfully applied in Poria cocos and Semen Coicis sample. Conclusions It is the first report on OPP detection by using CFP and doped nanozyme. The successful application in real sample indicates this method is a rapid, reliable strategy to detect OPP in Chinese herbs. |
| first_indexed | 2024-12-10T12:59:49Z |
| format | Article |
| id | doaj.art-c66cae5608404ae29383f2fc5c663873 |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-10T12:59:49Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-c66cae5608404ae29383f2fc5c6638732022-12-22T01:48:00ZengBMCChinese Medicine1749-85462020-03-011511710.1186/s13020-020-00304-2A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozymeJinchao Wei0Yan Xue1Jiayi Dong2Shuangpeng Wang3Hao Hu4Hao Gao5Peng Li6Yitao Wang7Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan UniversityState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauInstitute of Applied Physics and Materials Engineering, University of MacauInstitute of Applied Physics and Materials Engineering, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauInstitute of Traditional Chinese Medicine & Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan UniversityState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauAbstract Background Chinese herbs have been widely used for thousands of years. In order to kill or control insects and fungus during the cultivation of herb plants, pesticides have played a significant role. More than 30 kinds of pesticides have been documented in the latest version of Chinese Pharmacopoeia. It is urgent to develop new analytical methods for pesticide detection. Methods A fluorescent detection system was established by using Cerium based fluorescent polymer and Sm-CeO2. As a new doped nanozyme, Sm-CeO2 exhibits OPH-like activity to hydrolyze OPP pesticide. Results The morphology of the prepared CFP and Sm-CeO2 were characterized. The optimal conditions for CFP synthesis are CeCl3 (16 mmol L−1, 200 μL), ATP (4 mmol L−1, 200 μL) and Tris buffer (5 mmol L−1, 500 μL, pH 8.0). Sm-CeO2 shows the best mimic activity to hydrolyze OPP pesticide at pH = 8.0. The results exhibit good linear relationship between fluorescent quenching effect and MP in the range of 2–50 μmol L−1. Finally, this fluorescent technique was successfully applied in Poria cocos and Semen Coicis sample. Conclusions It is the first report on OPP detection by using CFP and doped nanozyme. The successful application in real sample indicates this method is a rapid, reliable strategy to detect OPP in Chinese herbs.http://link.springer.com/article/10.1186/s13020-020-00304-2NanozymeOrganophosphorusPesticideOrganophosphorus hydrolasePara-nitrophenolCerium oxide |
| spellingShingle | Jinchao Wei Yan Xue Jiayi Dong Shuangpeng Wang Hao Hu Hao Gao Peng Li Yitao Wang A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme Chinese Medicine Nanozyme Organophosphorus Pesticide Organophosphorus hydrolase Para-nitrophenol Cerium oxide |
| title | A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme |
| title_full | A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme |
| title_fullStr | A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme |
| title_full_unstemmed | A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme |
| title_short | A new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme |
| title_sort | new fluorescent technique for pesticide detection by using metal coordination polymer and nanozyme |
| topic | Nanozyme Organophosphorus Pesticide Organophosphorus hydrolase Para-nitrophenol Cerium oxide |
| url | http://link.springer.com/article/10.1186/s13020-020-00304-2 |
| work_keys_str_mv | AT jinchaowei anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT yanxue anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT jiayidong anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT shuangpengwang anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT haohu anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT haogao anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT pengli anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT yitaowang anewfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT jinchaowei newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT yanxue newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT jiayidong newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT shuangpengwang newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT haohu newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT haogao newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT pengli newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme AT yitaowang newfluorescenttechniqueforpesticidedetectionbyusingmetalcoordinationpolymerandnanozyme |