δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a
Objective To determine the effects of δ-tocotrienol on the proliferation of cervical carcinoma HeLa cells and whether miR-34a is involved in this process. Methods The expression of miR-34a and proliferation-related genes, cell viability, the protein levels of proliferation-related genes and cell cyc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2018-12-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0523.htm |
| _version_ | 1819071711704776704 |
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| author | YU Ying HUANG Jing LI Rong LIU Lianbin WANG Qi LUO Deping |
| author_facet | YU Ying HUANG Jing LI Rong LIU Lianbin WANG Qi LUO Deping |
| author_sort | YU Ying |
| collection | DOAJ |
| description | Objective To determine the effects of δ-tocotrienol on the proliferation of cervical carcinoma HeLa cells and whether miR-34a is involved in this process. Methods The expression of miR-34a and proliferation-related genes, cell viability, the protein levels of proliferation-related genes and cell cycle were examined using Real-time PCR, CCK-8 assay, Western blot and flow cytometry, respectively. miR-34a mimics and inhibitors were used to determine whether miR-34a mediated the effects of δ-tocotrienol on cell proliferation. Results δ-tocotrienol treatment for 36h significantly decreased the viability of HeLa cells in a dose-dependent manner. δ-tocotrienol treatment at 20 μmol/L-1 for 36h significantly stimulated miR-34a expression, however, inhibited CCND1 expression; meanwhile, cells proportion in S phase was decreased. Besides, after miR-34a mimics transfection, the cell viability and CCND1 expression were also decreased. Furthermore, miR-34a inhibitor transfection attenuated the inhibitory effects of δ-tocotrienol on cell proliferation. Conclusion δ-tocotrienol inhibits the proliferation of cervical carcinoma cells through upregulating miR-34a expression. |
| first_indexed | 2024-12-21T17:26:11Z |
| format | Article |
| id | doaj.art-c66da94bc9f249a7bfb1e61bc06683cb |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-21T17:26:11Z |
| publishDate | 2018-12-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-c66da94bc9f249a7bfb1e61bc06683cb2022-12-21T18:56:03ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782018-12-01451296596910.3971/j.issn.1000-8578.2018.18.05238578.2018.18.0523δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34aYU Ying0HUANG Jing1LI Rong2LIU Lianbin3WANG Qi4LUO Deping5Department of Gynecologic Oncology, Ganzhou Cancer Hospital, Ganzhou 341000, ChinaDepartment of Gynecologic Oncology, Ganzhou Cancer Hospital, Ganzhou 341000, ChinaDepartment of Gynecologic Oncology, Ganzhou Cancer Hospital, Ganzhou 341000, ChinaDepartment of Gynecologic Oncology, Ganzhou Cancer Hospital, Ganzhou 341000, ChinaDepartment of Gynecologic Oncology, Ganzhou Cancer Hospital, Ganzhou 341000, ChinaDepartment of Gynecologic Oncology, Ganzhou Cancer Hospital, Ganzhou 341000, ChinaObjective To determine the effects of δ-tocotrienol on the proliferation of cervical carcinoma HeLa cells and whether miR-34a is involved in this process. Methods The expression of miR-34a and proliferation-related genes, cell viability, the protein levels of proliferation-related genes and cell cycle were examined using Real-time PCR, CCK-8 assay, Western blot and flow cytometry, respectively. miR-34a mimics and inhibitors were used to determine whether miR-34a mediated the effects of δ-tocotrienol on cell proliferation. Results δ-tocotrienol treatment for 36h significantly decreased the viability of HeLa cells in a dose-dependent manner. δ-tocotrienol treatment at 20 μmol/L-1 for 36h significantly stimulated miR-34a expression, however, inhibited CCND1 expression; meanwhile, cells proportion in S phase was decreased. Besides, after miR-34a mimics transfection, the cell viability and CCND1 expression were also decreased. Furthermore, miR-34a inhibitor transfection attenuated the inhibitory effects of δ-tocotrienol on cell proliferation. Conclusion δ-tocotrienol inhibits the proliferation of cervical carcinoma cells through upregulating miR-34a expression.http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0523.htmcervical carcinomaδ-tocotrienolmir-34aproliferationccnd1 |
| spellingShingle | YU Ying HUANG Jing LI Rong LIU Lianbin WANG Qi LUO Deping δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a Zhongliu Fangzhi Yanjiu cervical carcinoma δ-tocotrienol mir-34a proliferation ccnd1 |
| title | δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a |
| title_full | δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a |
| title_fullStr | δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a |
| title_full_unstemmed | δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a |
| title_short | δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a |
| title_sort | δ tocotrienol inhibits proliferation of cervical carcinoma cells through upregulating microrna 34a |
| topic | cervical carcinoma δ-tocotrienol mir-34a proliferation ccnd1 |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0523.htm |
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