Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts
The aim of this work was to study the in vitro antibacterial activity possessed by killer yeast strains against bacteria contaminating alcoholic fermentation (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), in cell X cell and cell X crude toxin pre...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2010-10-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500006 |
| _version_ | 1819142947048783872 |
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| author | Maria Cristina Meneghin Vanda Renata Reis Sandra Regina Ceccato-Antonini |
| author_facet | Maria Cristina Meneghin Vanda Renata Reis Sandra Regina Ceccato-Antonini |
| author_sort | Maria Cristina Meneghin |
| collection | DOAJ |
| description | The aim of this work was to study the in vitro antibacterial activity possessed by killer yeast strains against bacteria contaminating alcoholic fermentation (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), in cell X cell and cell X crude toxin preparations. The bacteria were not inhibited by any S. cerevisiae killer strains (5 out of 11). The inhibition caused by two crude toxin preparations (Trichosporon figueirae and Candida sp) against L. plantarum was surprisingly high but not in the same extent for B. subtilis, especially with three killer strains (Candida glabrata, Pichia anomala and Candida sp). L. mesenteroides and L. fermentum strains were neither inhibited in cell X cell nor crude toxin X cell tests. The results suggested that killer activity of yeasts might operate over bacteria and it could be used for the biocontrol of contaminating bacteria from alcoholic fermentation if additional tests on toxin application in fermentation shown to be successful. A wider panel of S. cerevisiae killer strains should be used to confirm that they were really unable to control the growth of these Gram-positive bacteria.<br>Este estudo mostrou a atividade antibacteriana in vitro de linhagens de leveduras killer contra bactérias contaminantes da fermentação alcoólica (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), em testes célula X célula e célula X toxina bruta. As bactérias não foram inibidas por linhagens killer de Saccharomyces cerevisiae (5 dentre 11). Os preparados brutos de toxina de duas leveduras (Trichosporon figueirae e Candida sp) causaram uma alta inibição no crescimento de L. plantarum, mas não na mesma extensão para B. subtilis, especialmente para três leveduras killer (Candida glabrata, Pichia anomala e Candida sp). Linhagens de L. mesenteroides e L. fermentum não foram inibidas em nenhum dos testes. Os resultados obtidos neste estudo sugerem a ação de toxinas killer de leveduras contra bactérias, a qual poderia ser utilizada para o biocontrole de bactérias contaminantes da fermentação alcoólica se testes posteriores de aplicação da toxina dentro das dornas de fermentação se mostrarem eficientes. Um número maior de linhagens killer de S. cerevisiae deveria ser utilizado para confirmar se elas realmente são incapazes de controlar o crescimento destas bactérias Gram-positivas. |
| first_indexed | 2024-12-22T12:18:26Z |
| format | Article |
| id | doaj.art-c66e2c8f897740ca8741ffb0c3265190 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-22T12:18:26Z |
| publishDate | 2010-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-c66e2c8f897740ca8741ffb0c32651902022-12-21T18:26:04ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-10-015351043105010.1590/S1516-89132010000500006Inhibition of bacteria contaminating alcoholic fermentations by killer yeastsMaria Cristina MeneghinVanda Renata ReisSandra Regina Ceccato-AntoniniThe aim of this work was to study the in vitro antibacterial activity possessed by killer yeast strains against bacteria contaminating alcoholic fermentation (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), in cell X cell and cell X crude toxin preparations. The bacteria were not inhibited by any S. cerevisiae killer strains (5 out of 11). The inhibition caused by two crude toxin preparations (Trichosporon figueirae and Candida sp) against L. plantarum was surprisingly high but not in the same extent for B. subtilis, especially with three killer strains (Candida glabrata, Pichia anomala and Candida sp). L. mesenteroides and L. fermentum strains were neither inhibited in cell X cell nor crude toxin X cell tests. The results suggested that killer activity of yeasts might operate over bacteria and it could be used for the biocontrol of contaminating bacteria from alcoholic fermentation if additional tests on toxin application in fermentation shown to be successful. A wider panel of S. cerevisiae killer strains should be used to confirm that they were really unable to control the growth of these Gram-positive bacteria.<br>Este estudo mostrou a atividade antibacteriana in vitro de linhagens de leveduras killer contra bactérias contaminantes da fermentação alcoólica (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), em testes célula X célula e célula X toxina bruta. As bactérias não foram inibidas por linhagens killer de Saccharomyces cerevisiae (5 dentre 11). Os preparados brutos de toxina de duas leveduras (Trichosporon figueirae e Candida sp) causaram uma alta inibição no crescimento de L. plantarum, mas não na mesma extensão para B. subtilis, especialmente para três leveduras killer (Candida glabrata, Pichia anomala e Candida sp). Linhagens de L. mesenteroides e L. fermentum não foram inibidas em nenhum dos testes. Os resultados obtidos neste estudo sugerem a ação de toxinas killer de leveduras contra bactérias, a qual poderia ser utilizada para o biocontrole de bactérias contaminantes da fermentação alcoólica se testes posteriores de aplicação da toxina dentro das dornas de fermentação se mostrarem eficientes. Um número maior de linhagens killer de S. cerevisiae deveria ser utilizado para confirmar se elas realmente são incapazes de controlar o crescimento destas bactérias Gram-positivas.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500006Bacteriakiller yeastsalcoholfermentation |
| spellingShingle | Maria Cristina Meneghin Vanda Renata Reis Sandra Regina Ceccato-Antonini Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts Brazilian Archives of Biology and Technology Bacteria killer yeasts alcohol fermentation |
| title | Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts |
| title_full | Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts |
| title_fullStr | Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts |
| title_full_unstemmed | Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts |
| title_short | Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts |
| title_sort | inhibition of bacteria contaminating alcoholic fermentations by killer yeasts |
| topic | Bacteria killer yeasts alcohol fermentation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500006 |
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