Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacae
Bacterial biofilms are clinically admissible and illustrate an influential role in infections, particularly those related to the implant of medical devices. The characterization of biofilms is important to understand the etiology of the diseases. Enterobacter cloacae are known for causing infections...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.877060/full |
| _version_ | 1818116740860608512 |
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| author | Tripti Misra Meghana Tare Prabhat Nath Jha |
| author_facet | Tripti Misra Meghana Tare Prabhat Nath Jha |
| author_sort | Tripti Misra |
| collection | DOAJ |
| description | Bacterial biofilms are clinically admissible and illustrate an influential role in infections, particularly those related to the implant of medical devices. The characterization of biofilms is important to understand the etiology of the diseases. Enterobacter cloacae are known for causing infections by forming biofilms on various abiotic surfaces, such as medical devices. However, a detailed characterization in terms of morphology and the molecular composition of the formed biofilms by this bacterium is sparse. The present study provides insights into the biofilm formation of E. cloacae SBP-8, an environmental isolate, on various surfaces. We performed assays to understand the biofilm-forming capability of the SBP-8 strain and characterized the adhering potential of the bacteria on the surface of different medical devices (foley latex catheter, enteral feeding tube, and glass) at different temperatures. We found that medical devices exhibited strong colonization by E. cloacae SBP-8. Using field emission-scanning electron microscopy (FE-SEM) studies, we characterized the biofilms as a function of time. It indicated stronger biofilm formation in terms of cellular density and EPS production on the surfaces. Further, we characterized the biofilm employing surface-enhanced Raman spectroscopy (SERS) and identified the vast heterogenic nature of the biofilm-forming molecules. Interestingly, we also found that this heterogeneity varies from the initial stages of biofilm formation until the maturation and dispersion. Our studies provide insights into biofilm composition over a period of time, which might aid in understanding the biofilm dispersion phases, to enhance the presently available treatment strategies. |
| first_indexed | 2024-12-11T04:27:19Z |
| format | Article |
| id | doaj.art-c66e543877074120976b296e946e4dd2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T04:27:19Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c66e543877074120976b296e946e4dd22022-12-22T01:20:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.877060877060Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacaeTripti MisraMeghana TarePrabhat Nath JhaBacterial biofilms are clinically admissible and illustrate an influential role in infections, particularly those related to the implant of medical devices. The characterization of biofilms is important to understand the etiology of the diseases. Enterobacter cloacae are known for causing infections by forming biofilms on various abiotic surfaces, such as medical devices. However, a detailed characterization in terms of morphology and the molecular composition of the formed biofilms by this bacterium is sparse. The present study provides insights into the biofilm formation of E. cloacae SBP-8, an environmental isolate, on various surfaces. We performed assays to understand the biofilm-forming capability of the SBP-8 strain and characterized the adhering potential of the bacteria on the surface of different medical devices (foley latex catheter, enteral feeding tube, and glass) at different temperatures. We found that medical devices exhibited strong colonization by E. cloacae SBP-8. Using field emission-scanning electron microscopy (FE-SEM) studies, we characterized the biofilms as a function of time. It indicated stronger biofilm formation in terms of cellular density and EPS production on the surfaces. Further, we characterized the biofilm employing surface-enhanced Raman spectroscopy (SERS) and identified the vast heterogenic nature of the biofilm-forming molecules. Interestingly, we also found that this heterogeneity varies from the initial stages of biofilm formation until the maturation and dispersion. Our studies provide insights into biofilm composition over a period of time, which might aid in understanding the biofilm dispersion phases, to enhance the presently available treatment strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2022.877060/fullbiofilmsSERSEnterobacter cloacaeFESEMmedical device |
| spellingShingle | Tripti Misra Meghana Tare Prabhat Nath Jha Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacae Frontiers in Microbiology biofilms SERS Enterobacter cloacae FESEM medical device |
| title | Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacae |
| title_full | Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacae |
| title_fullStr | Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacae |
| title_full_unstemmed | Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacae |
| title_short | Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of Enterobacter cloacae |
| title_sort | insights into the dynamics and composition of biofilm formed by environmental isolate of enterobacter cloacae |
| topic | biofilms SERS Enterobacter cloacae FESEM medical device |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.877060/full |
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