Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy
Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-07-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/20/13/3275 |
| _version_ | 1811198136071225344 |
|---|---|
| author | Jagoba Iturri Andreas Weber Alberto Moreno-Cencerrado Maria dM Vivanco Rafael Benítez Stefano Leporatti José Luis Toca-Herrera |
| author_facet | Jagoba Iturri Andreas Weber Alberto Moreno-Cencerrado Maria dM Vivanco Rafael Benítez Stefano Leporatti José Luis Toca-Herrera |
| author_sort | Jagoba Iturri |
| collection | DOAJ |
| description | Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young’s modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0−6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment. |
| first_indexed | 2024-04-12T01:25:43Z |
| format | Article |
| id | doaj.art-c671e2946af14e53ac85fd9a1e9991f4 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T01:25:43Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c671e2946af14e53ac85fd9a1e9991f42022-12-22T03:53:40ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-07-012013327510.3390/ijms20133275ijms20133275Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force MicroscopyJagoba Iturri0Andreas Weber1Alberto Moreno-Cencerrado2Maria dM Vivanco3Rafael Benítez4Stefano Leporatti5José Luis Toca-Herrera6Institute for Biophysics, Department of Nanobiotechnology (DNBT), BOKU University for Natural Resources and Life Sciences, Muthgasse 11 (Simon Zeisel Haus), A-1190 Vienna, AustriaInstitute for Biophysics, Department of Nanobiotechnology (DNBT), BOKU University for Natural Resources and Life Sciences, Muthgasse 11 (Simon Zeisel Haus), A-1190 Vienna, AustriaInstitute for Biophysics, Department of Nanobiotechnology (DNBT), BOKU University for Natural Resources and Life Sciences, Muthgasse 11 (Simon Zeisel Haus), A-1190 Vienna, AustriaCancer Heterogeneity Lab, CIC bioGUNE, Bizkaia Science and Technology Park, 48160 Derio, SpainDepartment Matemáticas para la Economía y la Empresa, Facultad de Economía, Universidad de Valencia, Avda. Tarongers s/n, 46022 Valencia, SpainCNR Nanotec-Istituto di Nanotecnologia, Polo di Nanotecnologia c/o Campus Ecoteckne, Via Monteroni, 73100 Lecce, ItalyInstitute for Biophysics, Department of Nanobiotechnology (DNBT), BOKU University for Natural Resources and Life Sciences, Muthgasse 11 (Simon Zeisel Haus), A-1190 Vienna, AustriaAtomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young’s modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0−6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment.https://www.mdpi.com/1422-0067/20/13/3275resveratrolMCF-7cytomechanicsatomic force microscopyforce spectroscopyfluorescence microscopy |
| spellingShingle | Jagoba Iturri Andreas Weber Alberto Moreno-Cencerrado Maria dM Vivanco Rafael Benítez Stefano Leporatti José Luis Toca-Herrera Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy International Journal of Molecular Sciences resveratrol MCF-7 cytomechanics atomic force microscopy force spectroscopy fluorescence microscopy |
| title | Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy |
| title_full | Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy |
| title_fullStr | Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy |
| title_full_unstemmed | Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy |
| title_short | Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy |
| title_sort | resveratrol induced temporal variation in the mechanical properties of mcf 7 breast cancer cells investigated by atomic force microscopy |
| topic | resveratrol MCF-7 cytomechanics atomic force microscopy force spectroscopy fluorescence microscopy |
| url | https://www.mdpi.com/1422-0067/20/13/3275 |
| work_keys_str_mv | AT jagobaiturri resveratrolinducedtemporalvariationinthemechanicalpropertiesofmcf7breastcancercellsinvestigatedbyatomicforcemicroscopy AT andreasweber resveratrolinducedtemporalvariationinthemechanicalpropertiesofmcf7breastcancercellsinvestigatedbyatomicforcemicroscopy AT albertomorenocencerrado resveratrolinducedtemporalvariationinthemechanicalpropertiesofmcf7breastcancercellsinvestigatedbyatomicforcemicroscopy AT mariadmvivanco resveratrolinducedtemporalvariationinthemechanicalpropertiesofmcf7breastcancercellsinvestigatedbyatomicforcemicroscopy AT rafaelbenitez resveratrolinducedtemporalvariationinthemechanicalpropertiesofmcf7breastcancercellsinvestigatedbyatomicforcemicroscopy AT stefanoleporatti resveratrolinducedtemporalvariationinthemechanicalpropertiesofmcf7breastcancercellsinvestigatedbyatomicforcemicroscopy AT joseluistocaherrera resveratrolinducedtemporalvariationinthemechanicalpropertiesofmcf7breastcancercellsinvestigatedbyatomicforcemicroscopy |