Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance setting
Background: Rapid diagnosis of drug-resistant Mycobacterium tuberculosis is a challenge in low-income countries. Phenotypic drug susceptibility testing using Sensititre® MycoTB assay and the resazurin microtitre plate assay (REMA) are relatively new innovative methods to determine drug susceptibilit...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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AOSIS
2019-12-01
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| Series: | African Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | https://ajlmonline.org/index.php/ajlm/article/view/840 |
| _version_ | 1818008457340518400 |
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| author | Prenika Jaglal Melendhran Pillay Koleka Mlisana |
| author_facet | Prenika Jaglal Melendhran Pillay Koleka Mlisana |
| author_sort | Prenika Jaglal |
| collection | DOAJ |
| description | Background: Rapid diagnosis of drug-resistant Mycobacterium tuberculosis is a challenge in low-income countries. Phenotypic drug susceptibility testing using Sensititre® MycoTB assay and the resazurin microtitre plate assay (REMA) are relatively new innovative methods to determine drug susceptibility.
Objectives: This study aimed to determine the performance of the Sensititre and REMA for M. tuberculosis drug susceptibility testing in a high-volume tuberculosis reference laboratory.
Methods: A laboratory-based study was performed at the Inkosi Albert Luthuli Central Hospital Tuberculosis Laboratory from January 2014 to June 2015. The Sensititre® MycoTB plate and REMA were compared to the gold standard agar proportion method (APM) using 134 stored isolates.
Results: Agreement between the Sensititre® MycoTB plate and APM was observed with 98% sensitivity, 82% specificity, 94% positive and 93% negative predictive values of the Sensititre® MycoTB assay for the detection of rifampicin resistance and 97%, 96%, 99% and 88% for isoniazid resistance. Good categorical agreement between the REMA and the APM was observed among isolates with 89% sensitivity, 68% specificity, 89% positive and 68% negative predictive value for the detection of rifampicin resistance and 95%, 96%, 99% and 81% for isoniazid resistance. Results for the second-line drugs showed elevated minimum inhibitory concentrations for multidrug-resistant and extensively drug-resistant tuberculosis isolates.
Conclusion: The REMA and Sensititre® MycoTB plate are attractive alternatives to the gold standard APM for the phenotypic detection of M. tuberculosis drug resistance. |
| first_indexed | 2024-04-14T05:29:29Z |
| format | Article |
| id | doaj.art-c672a08774814c77ba74ac1e997ff5e5 |
| institution | Directory Open Access Journal |
| issn | 2225-2002 2225-2010 |
| language | English |
| last_indexed | 2024-04-14T05:29:29Z |
| publishDate | 2019-12-01 |
| publisher | AOSIS |
| record_format | Article |
| series | African Journal of Laboratory Medicine |
| spelling | doaj.art-c672a08774814c77ba74ac1e997ff5e52022-12-22T02:09:51ZengAOSISAfrican Journal of Laboratory Medicine2225-20022225-20102019-12-0181e1e910.4102/ajlm.v8i1.840248Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance settingPrenika Jaglal0Melendhran Pillay1Koleka Mlisana2Department of Medical Microbiology, National Health Laboratory Services, School of Laboratory Medicine and Medical Science, University of KwaZulu-Natal, DurbanDepartment of Medical Microbiology/Virology, National Health Laboratory Service, DurbanDepartment of Medical Microbiology, National Health Laboratory Services, School of Laboratory Medicine and Medical Science, University of KwaZulu-Natal, DurbanBackground: Rapid diagnosis of drug-resistant Mycobacterium tuberculosis is a challenge in low-income countries. Phenotypic drug susceptibility testing using Sensititre® MycoTB assay and the resazurin microtitre plate assay (REMA) are relatively new innovative methods to determine drug susceptibility. Objectives: This study aimed to determine the performance of the Sensititre and REMA for M. tuberculosis drug susceptibility testing in a high-volume tuberculosis reference laboratory. Methods: A laboratory-based study was performed at the Inkosi Albert Luthuli Central Hospital Tuberculosis Laboratory from January 2014 to June 2015. The Sensititre® MycoTB plate and REMA were compared to the gold standard agar proportion method (APM) using 134 stored isolates. Results: Agreement between the Sensititre® MycoTB plate and APM was observed with 98% sensitivity, 82% specificity, 94% positive and 93% negative predictive values of the Sensititre® MycoTB assay for the detection of rifampicin resistance and 97%, 96%, 99% and 88% for isoniazid resistance. Good categorical agreement between the REMA and the APM was observed among isolates with 89% sensitivity, 68% specificity, 89% positive and 68% negative predictive value for the detection of rifampicin resistance and 95%, 96%, 99% and 81% for isoniazid resistance. Results for the second-line drugs showed elevated minimum inhibitory concentrations for multidrug-resistant and extensively drug-resistant tuberculosis isolates. Conclusion: The REMA and Sensititre® MycoTB plate are attractive alternatives to the gold standard APM for the phenotypic detection of M. tuberculosis drug resistance.https://ajlmonline.org/index.php/ajlm/article/view/840mycobacterium tuberculosisagar proportion methodmultidrug-resistant tuberculosisextensively drug-resistant tuberculosissensititre® mycotb assayresazurin microtitre plate assay |
| spellingShingle | Prenika Jaglal Melendhran Pillay Koleka Mlisana Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance setting African Journal of Laboratory Medicine mycobacterium tuberculosis agar proportion method multidrug-resistant tuberculosis extensively drug-resistant tuberculosis sensititre® mycotb assay resazurin microtitre plate assay |
| title | Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance setting |
| title_full | Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance setting |
| title_fullStr | Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance setting |
| title_full_unstemmed | Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance setting |
| title_short | Resazurin microtitre plate assay and Sensititre® MycoTB for detection of Mycobacterium tuberculosis resistance in a high tuberculosis resistance setting |
| title_sort | resazurin microtitre plate assay and sensititre r mycotb for detection of mycobacterium tuberculosis resistance in a high tuberculosis resistance setting |
| topic | mycobacterium tuberculosis agar proportion method multidrug-resistant tuberculosis extensively drug-resistant tuberculosis sensititre® mycotb assay resazurin microtitre plate assay |
| url | https://ajlmonline.org/index.php/ajlm/article/view/840 |
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