Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients
Abstract Programmed cell death ligand 1 protein-positive (PD-L1+) exosomes have been found to be a potential biomarker for the diagnosis of non-small cell lung cancer (NSCLC). However, the development of highly sensitive detection technique for PD-L1+ exosomes is still a challenge in clinical applic...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-01845-y |
| _version_ | 1797863698119786496 |
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| author | Luyue Chang Haiping Wu Rui Chen Xiaoqing Sun Yi Yang Changwu Huang Shijia Ding Changjin Liu Wei Cheng |
| author_facet | Luyue Chang Haiping Wu Rui Chen Xiaoqing Sun Yi Yang Changwu Huang Shijia Ding Changjin Liu Wei Cheng |
| author_sort | Luyue Chang |
| collection | DOAJ |
| description | Abstract Programmed cell death ligand 1 protein-positive (PD-L1+) exosomes have been found to be a potential biomarker for the diagnosis of non-small cell lung cancer (NSCLC). However, the development of highly sensitive detection technique for PD-L1+ exosomes is still a challenge in clinical applications. Herein, a sandwich electrochemical aptasensor based on ternary metal-metalloid palladium-copper-boron alloy microporous nanospheres (PdCuB MNs) and Au@CuCl2 nanowires (NWs) was designed for the detection of PD-L1+ exosomes. The excellent peroxidase-like catalytic activity of PdCuB MNs and the high conductivity of Au@CuCl2 NWs endow the fabricated aptasensor with intense electrochemical signal, thus enabling the detection of low abundance exosomes. The analytical results revealed that the aptasensor maintained favorable linearity over a wide concentration range of 6 orders of magnitude and reached a low detection limit of 36 particles/mL. The aptasensor is successfully applied to the analysis of complex serum samples and achieves the accurate identification of clinical NSCLC patients. Overall, the developed electrochemical aptasensor provides a powerful tool for early diagnosis of NSCLC. |
| first_indexed | 2024-04-09T22:39:42Z |
| format | Article |
| id | doaj.art-c672a87db687431c8c3a10f456f762cf |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-09T22:39:42Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-c672a87db687431c8c3a10f456f762cf2023-03-22T12:16:28ZengBMCJournal of Nanobiotechnology1477-31552023-03-0121111110.1186/s12951-023-01845-yMicroporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patientsLuyue Chang0Haiping Wu1Rui Chen2Xiaoqing Sun3Yi Yang4Changwu Huang5Shijia Ding6Changjin Liu7Wei Cheng8The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical UniversityKey Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical UniversityKey Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical UniversityDepartment of Laboratory Medicine, The Fifth People’s Hospital of ChongqingDepartment of Laboratory Medicine, The Fifth People’s Hospital of ChongqingDepartment of Laboratory Medicine, The Fifth People’s Hospital of ChongqingKey Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical UniversityThe Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical UniversityThe Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical UniversityAbstract Programmed cell death ligand 1 protein-positive (PD-L1+) exosomes have been found to be a potential biomarker for the diagnosis of non-small cell lung cancer (NSCLC). However, the development of highly sensitive detection technique for PD-L1+ exosomes is still a challenge in clinical applications. Herein, a sandwich electrochemical aptasensor based on ternary metal-metalloid palladium-copper-boron alloy microporous nanospheres (PdCuB MNs) and Au@CuCl2 nanowires (NWs) was designed for the detection of PD-L1+ exosomes. The excellent peroxidase-like catalytic activity of PdCuB MNs and the high conductivity of Au@CuCl2 NWs endow the fabricated aptasensor with intense electrochemical signal, thus enabling the detection of low abundance exosomes. The analytical results revealed that the aptasensor maintained favorable linearity over a wide concentration range of 6 orders of magnitude and reached a low detection limit of 36 particles/mL. The aptasensor is successfully applied to the analysis of complex serum samples and achieves the accurate identification of clinical NSCLC patients. Overall, the developed electrochemical aptasensor provides a powerful tool for early diagnosis of NSCLC.https://doi.org/10.1186/s12951-023-01845-yPD-L1+ exosomeElectrochemical aptasensorMicroporous PdCuB nanotagAu@CuCl2 nanowiresNon-small cell lung cancer |
| spellingShingle | Luyue Chang Haiping Wu Rui Chen Xiaoqing Sun Yi Yang Changwu Huang Shijia Ding Changjin Liu Wei Cheng Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients Journal of Nanobiotechnology PD-L1+ exosome Electrochemical aptasensor Microporous PdCuB nanotag Au@CuCl2 nanowires Non-small cell lung cancer |
| title | Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients |
| title_full | Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients |
| title_fullStr | Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients |
| title_full_unstemmed | Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients |
| title_short | Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients |
| title_sort | microporous pdcub nanotag based electrochemical aptasensor with au cucl2 nanowires interface for ultrasensitive detection of pd l1 positive exosomes in the serum of lung cancer patients |
| topic | PD-L1+ exosome Electrochemical aptasensor Microporous PdCuB nanotag Au@CuCl2 nanowires Non-small cell lung cancer |
| url | https://doi.org/10.1186/s12951-023-01845-y |
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