Peripheral Blood Gene Expression Profile of Infants with Atopic Dermatitis
To enhance the understanding of molecular mechanisms and mine previously unidentified biomarkers of pediatric atopic dermatitis, PBMC gene expression profiles were generated by RNA sequencing in infants with atopic dermatitis and age-matched controls. A total of 178 significantly differentially expr...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | JID Innovations |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266702672200073X |
| _version_ | 1797856441857474560 |
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| author | Janna Nousbeck Maeve A. McAleer Alan D. Irvine |
| author_facet | Janna Nousbeck Maeve A. McAleer Alan D. Irvine |
| author_sort | Janna Nousbeck |
| collection | DOAJ |
| description | To enhance the understanding of molecular mechanisms and mine previously unidentified biomarkers of pediatric atopic dermatitis, PBMC gene expression profiles were generated by RNA sequencing in infants with atopic dermatitis and age-matched controls. A total of 178 significantly differentially expressed genes (DEGs) (115 upregulations and 63 downregulations) were seen, compared with those in healthy controls. The DEGs identified included IL1β, TNF, TREM1, IL18R1, and IL18RAP. DEGs were validated by real-time RT- qPCR in a larger number of samples from PBMCs of infants with atopic dermatitis aged <12 months. Using the DAVID (Database for Annotation, Visualization and Integrated Discovery) database, functional and pathway enrichment analyses of DEGs were performed. Gene ontology enrichment analysis showed that DEGs were associated with immune responses, inflammatory responses, regulation of immune responses, and platelet activation. Pathway analysis indicated that DEGs were enriched in cytokine‒cytokine receptor interaction, immunoregulatory interactions between lymphoid and nonlymphoid cells, hematopoietic cell lineage, phosphoinositide 3-kinase‒protein kinase B signaling pathway, NK cell‒mediated cytotoxicity, and platelet activation. Furthermore, the protein‒protein interaction network was predicted using the STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) database and visualized with Cytoscape software. Finally, on the basis of the protein‒protein interaction network, 18 hub genes were selected, and two significant modules were obtained. In conclusion, this study sheds light on the molecular mechanisms of pediatric atopic dermatitis and may provide diagnostic biomarkers and therapeutic targets. |
| first_indexed | 2024-04-09T20:40:30Z |
| format | Article |
| id | doaj.art-c6744b1040044ab1a5d4abb78049bf88 |
| institution | Directory Open Access Journal |
| issn | 2667-0267 |
| language | English |
| last_indexed | 2024-04-09T20:40:30Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JID Innovations |
| spelling | doaj.art-c6744b1040044ab1a5d4abb78049bf882023-03-30T04:27:19ZengElsevierJID Innovations2667-02672023-03-0132100165Peripheral Blood Gene Expression Profile of Infants with Atopic DermatitisJanna Nousbeck0Maeve A. McAleer1Alan D. Irvine2National Children’s Research Centre, Dublin, Ireland; Clinical Medicine, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin, IrelandNational Children’s Research Centre, Dublin, Ireland; Department of Paediatric Dermatology, Children’s Health Ireland at Crumlin, Dublin, IrelandNational Children’s Research Centre, Dublin, Ireland; Clinical Medicine, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin, Ireland; Department of Paediatric Dermatology, Children’s Health Ireland at Crumlin, Dublin, Ireland; Correspondence: Alan D. Irvine, Department of Paediatric Dermatology, Children’s Health Ireland at Crumlin, Dublin 12, Ireland.To enhance the understanding of molecular mechanisms and mine previously unidentified biomarkers of pediatric atopic dermatitis, PBMC gene expression profiles were generated by RNA sequencing in infants with atopic dermatitis and age-matched controls. A total of 178 significantly differentially expressed genes (DEGs) (115 upregulations and 63 downregulations) were seen, compared with those in healthy controls. The DEGs identified included IL1β, TNF, TREM1, IL18R1, and IL18RAP. DEGs were validated by real-time RT- qPCR in a larger number of samples from PBMCs of infants with atopic dermatitis aged <12 months. Using the DAVID (Database for Annotation, Visualization and Integrated Discovery) database, functional and pathway enrichment analyses of DEGs were performed. Gene ontology enrichment analysis showed that DEGs were associated with immune responses, inflammatory responses, regulation of immune responses, and platelet activation. Pathway analysis indicated that DEGs were enriched in cytokine‒cytokine receptor interaction, immunoregulatory interactions between lymphoid and nonlymphoid cells, hematopoietic cell lineage, phosphoinositide 3-kinase‒protein kinase B signaling pathway, NK cell‒mediated cytotoxicity, and platelet activation. Furthermore, the protein‒protein interaction network was predicted using the STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) database and visualized with Cytoscape software. Finally, on the basis of the protein‒protein interaction network, 18 hub genes were selected, and two significant modules were obtained. In conclusion, this study sheds light on the molecular mechanisms of pediatric atopic dermatitis and may provide diagnostic biomarkers and therapeutic targets.http://www.sciencedirect.com/science/article/pii/S266702672200073X |
| spellingShingle | Janna Nousbeck Maeve A. McAleer Alan D. Irvine Peripheral Blood Gene Expression Profile of Infants with Atopic Dermatitis JID Innovations |
| title | Peripheral Blood Gene Expression Profile of Infants with Atopic Dermatitis |
| title_full | Peripheral Blood Gene Expression Profile of Infants with Atopic Dermatitis |
| title_fullStr | Peripheral Blood Gene Expression Profile of Infants with Atopic Dermatitis |
| title_full_unstemmed | Peripheral Blood Gene Expression Profile of Infants with Atopic Dermatitis |
| title_short | Peripheral Blood Gene Expression Profile of Infants with Atopic Dermatitis |
| title_sort | peripheral blood gene expression profile of infants with atopic dermatitis |
| url | http://www.sciencedirect.com/science/article/pii/S266702672200073X |
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