Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approach
Background: Morinda citrifolia (Noni) is an herbal remedy with multiple therapeutic properties such as antioxidant, anticancer, anti-inflammatory, antihypertensive effects due to rich in phytochemical constituents. Purpose: To evaluate chemoprotective effect of noni in dipping cisplatin (CP)-induced...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031320300099 |
| _version_ | 1818574057215361024 |
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| author | Mohammad Ali SN Manjula Shahid Ud Din Wani Vipan K Parihar KM Mruthunjaya SubbaRao V Madhunapantula |
| author_facet | Mohammad Ali SN Manjula Shahid Ud Din Wani Vipan K Parihar KM Mruthunjaya SubbaRao V Madhunapantula |
| author_sort | Mohammad Ali |
| collection | DOAJ |
| description | Background: Morinda citrifolia (Noni) is an herbal remedy with multiple therapeutic properties such as antioxidant, anticancer, anti-inflammatory, antihypertensive effects due to rich in phytochemical constituents. Purpose: To evaluate chemoprotective effect of noni in dipping cisplatin (CP)-induced cytotoxicity in normal (healthy) cells through an antioxidant activity using in-vitro and in-vivo. Methods: Chemoprotective study of fresh noni juice (NJ) and divine noni gold (DNG) at the doses (0.3125–10 mg/ml) were performed against CP (3.125–100 µM) induced cytotoxicity in normal cells (BEAS-2B) by Sulforhodamine B assay at 24 h and 48 h and the percentage of growth inhibition was calculated. Similarly, the protective effect of noni (360 mg/b.wt.) were investigated against CP (5 mg/ml) induced cardiotoxicity in healthy mice through the investigation of serum biochemical markers, endogenous antioxidant enzymes were interpreted. Finally, the chemoprotective mechanism of DNG was accomplished Nrf2 expression following CP challenged NIH-3T3 cells was performed by the western blot method. Results: Treatment with noni at tested dose exhibited the least toxicity in normal cells (BEAS-2B). CP at the following concentrations induced cell death in time and dose-dependent manner. Whereas, noni revealed a significant cytoprotective effect against CP-induced cytotoxicity in the normal cells. Further in in-vivo study, treatment with noni at 360 mg/b.wt in CP injected animals attenuated cardiac injury as indicated by quenching the activities of serum biochemical markers, alleviated enzymatic and non-enzymatic antioxidant enzymes. Furthermore, noni attenuated CP induced toxicity by indirect antioxidant activity were proved by Nrf2 expression. Conclusion: Results suggest that DNG is more potent than NJ. Findings confirmed that DNG protects against CP-induced toxicity by activating the Nrf2 expression. More studies are needed to comprehend the mechanisms of DNG cytoprotection. |
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| format | Article |
| id | doaj.art-c6745fea66f54e389148dbd25e77d975 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-12-15T00:21:22Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-c6745fea66f54e389148dbd25e77d9752022-12-21T22:42:18ZengElsevierPhytomedicine Plus2667-03132021-02-0111100009Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approachMohammad Ali0SN Manjula1Shahid Ud Din Wani2Vipan K Parihar3KM Mruthunjaya4SubbaRao V Madhunapantula5Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru 570015, India; Corresponding author.Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru 570015, IndiaDepartment of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru 570015, IndiaDepartment of Radiation Oncology, University of California, Irvine, Senior Scientist at NASA Specialized Center of Research-UC Irvine, United StatesDepartment of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru 570015, IndiaDepartment of Biochemistry, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru 570015, IndiaBackground: Morinda citrifolia (Noni) is an herbal remedy with multiple therapeutic properties such as antioxidant, anticancer, anti-inflammatory, antihypertensive effects due to rich in phytochemical constituents. Purpose: To evaluate chemoprotective effect of noni in dipping cisplatin (CP)-induced cytotoxicity in normal (healthy) cells through an antioxidant activity using in-vitro and in-vivo. Methods: Chemoprotective study of fresh noni juice (NJ) and divine noni gold (DNG) at the doses (0.3125–10 mg/ml) were performed against CP (3.125–100 µM) induced cytotoxicity in normal cells (BEAS-2B) by Sulforhodamine B assay at 24 h and 48 h and the percentage of growth inhibition was calculated. Similarly, the protective effect of noni (360 mg/b.wt.) were investigated against CP (5 mg/ml) induced cardiotoxicity in healthy mice through the investigation of serum biochemical markers, endogenous antioxidant enzymes were interpreted. Finally, the chemoprotective mechanism of DNG was accomplished Nrf2 expression following CP challenged NIH-3T3 cells was performed by the western blot method. Results: Treatment with noni at tested dose exhibited the least toxicity in normal cells (BEAS-2B). CP at the following concentrations induced cell death in time and dose-dependent manner. Whereas, noni revealed a significant cytoprotective effect against CP-induced cytotoxicity in the normal cells. Further in in-vivo study, treatment with noni at 360 mg/b.wt in CP injected animals attenuated cardiac injury as indicated by quenching the activities of serum biochemical markers, alleviated enzymatic and non-enzymatic antioxidant enzymes. Furthermore, noni attenuated CP induced toxicity by indirect antioxidant activity were proved by Nrf2 expression. Conclusion: Results suggest that DNG is more potent than NJ. Findings confirmed that DNG protects against CP-induced toxicity by activating the Nrf2 expression. More studies are needed to comprehend the mechanisms of DNG cytoprotection.http://www.sciencedirect.com/science/article/pii/S2667031320300099AntioxidantToxicityCancerChemotherapyNoniCisplatin |
| spellingShingle | Mohammad Ali SN Manjula Shahid Ud Din Wani Vipan K Parihar KM Mruthunjaya SubbaRao V Madhunapantula Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approach Phytomedicine Plus Antioxidant Toxicity Cancer Chemotherapy Noni Cisplatin |
| title | Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approach |
| title_full | Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approach |
| title_fullStr | Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approach |
| title_full_unstemmed | Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approach |
| title_short | Protective role of herbal formulation-divine noni against cisplatin-induced cytotoxicity in healthy cells by activating Nrf2 expression: An in-vivo and in-vitro approach |
| title_sort | protective role of herbal formulation divine noni against cisplatin induced cytotoxicity in healthy cells by activating nrf2 expression an in vivo and in vitro approach |
| topic | Antioxidant Toxicity Cancer Chemotherapy Noni Cisplatin |
| url | http://www.sciencedirect.com/science/article/pii/S2667031320300099 |
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