CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathway
<p>Abstract</p> <p>Objective</p> <p>To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells <it>in vitro</it>, and to examine the effect of CpG-ODN treatment on th...
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| Format: | Article |
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BMC
2011-05-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
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| Online Access: | http://www.jeccr.com/content/30/1/48 |
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| author | Li Jing Fu Rongquan Zheng Jianfeng Wang Xiaozhong |
| author_facet | Li Jing Fu Rongquan Zheng Jianfeng Wang Xiaozhong |
| author_sort | Li Jing |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Objective</p> <p>To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells <it>in vitro</it>, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis <it>in vitro</it>.</p> <p>Methods</p> <p>The expressions of FasL in HepG2 and Fas in Jurkat cells were examined by real time PCR and flow cytometry (FCM). HepG2 and Jurkat cells were co-cultured, and the frequency of apoptotic Jurkat cells and levels of activated caspase-3 were determined by FCM.</p> <p>Results</p> <p>Treatment with CpG-ODN down-regulated the expression of FasL in HepG2 cells in a dose- and time-dependent manner. In addition, treatment with CpG-ODN down-regulated the Fas mRNA transcription and protein expression in Jurkat cells. Treatment of HepG2 cells or Jurkat cells with FasL-neutralizing antibody NOK-2 remarkably inhibited the HepG2-medaited Jurkat cell apoptosis. Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our data indicated that treatment with CpG-ODN inhibited the HepG2 cells-mediated Jurkat cell apoptosis by modulating the Fas/FasL pathway. Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC.</p> |
| first_indexed | 2024-12-11T10:32:45Z |
| format | Article |
| id | doaj.art-c675c998b48545c0a54a7f3ebccc398b |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-11T10:32:45Z |
| publishDate | 2011-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-c675c998b48545c0a54a7f3ebccc398b2022-12-22T01:10:52ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662011-05-013014810.1186/1756-9966-30-48CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathwayLi JingFu RongquanZheng JianfengWang Xiaozhong<p>Abstract</p> <p>Objective</p> <p>To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells <it>in vitro</it>, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis <it>in vitro</it>.</p> <p>Methods</p> <p>The expressions of FasL in HepG2 and Fas in Jurkat cells were examined by real time PCR and flow cytometry (FCM). HepG2 and Jurkat cells were co-cultured, and the frequency of apoptotic Jurkat cells and levels of activated caspase-3 were determined by FCM.</p> <p>Results</p> <p>Treatment with CpG-ODN down-regulated the expression of FasL in HepG2 cells in a dose- and time-dependent manner. In addition, treatment with CpG-ODN down-regulated the Fas mRNA transcription and protein expression in Jurkat cells. Treatment of HepG2 cells or Jurkat cells with FasL-neutralizing antibody NOK-2 remarkably inhibited the HepG2-medaited Jurkat cell apoptosis. Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our data indicated that treatment with CpG-ODN inhibited the HepG2 cells-mediated Jurkat cell apoptosis by modulating the Fas/FasL pathway. Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC.</p>http://www.jeccr.com/content/30/1/48CpG-ODNhepatocellular carcinomaapoptosis |
| spellingShingle | Li Jing Fu Rongquan Zheng Jianfeng Wang Xiaozhong CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathway Journal of Experimental & Clinical Cancer Research CpG-ODN hepatocellular carcinoma apoptosis |
| title | CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathway |
| title_full | CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathway |
| title_fullStr | CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathway |
| title_full_unstemmed | CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathway |
| title_short | CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis <it>via </it>the Fas-FasL-mediated pathway |
| title_sort | cpg oligonucleotides suppress hepg2 cells induced jurkat cell apoptosis it via it the fas fasl mediated pathway |
| topic | CpG-ODN hepatocellular carcinoma apoptosis |
| url | http://www.jeccr.com/content/30/1/48 |
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