Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage Temperatures
Mesenchymal stem cells play an important role in regenerative medicine due to their capability of self-renewal and multipotent differentiation. For research or clinical application, bone marrow aspirates are harvested during elective surgeries to isolate MSCs. If an immediate purification of the MSC...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/6864988 |
| _version_ | 1798031764123287552 |
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| author | Valentin Schrodi Claudia Neunaber Katrin Bundkirchen Weikang Ye Zhida Jiang Maximilian Petri Christian Krettek Sandra Noack |
| author_facet | Valentin Schrodi Claudia Neunaber Katrin Bundkirchen Weikang Ye Zhida Jiang Maximilian Petri Christian Krettek Sandra Noack |
| author_sort | Valentin Schrodi |
| collection | DOAJ |
| description | Mesenchymal stem cells play an important role in regenerative medicine due to their capability of self-renewal and multipotent differentiation. For research or clinical application, bone marrow aspirates are harvested during elective surgeries to isolate MSCs. If an immediate purification of the MSCs is not possible, the bone marrow must be stored. Therefore, the aim of this study was to investigate possible differences of stem cell characteristics regarding the self-renewal capability, the adipogenic, chondrogenic, and osteogenic differentiation, and the expression of surface antigens after different storage conditions of the bone marrow aspirates. Three groups were analysed: the first group was purified immediately after harvesting, the other two groups were processed after they were stored 18 to 24 hours at 22°C (room temperature) or at 4°C. Comparisons between the groups were performed using the Kruskal-Wallis test for nonparametric data. The final results showed no significant difference between the different storage conditions. Therefore, storage of bone marrow aspirates for 18 to 24 hours at room temperature or 4°C is possible without loss of stem cell characteristics. |
| first_indexed | 2024-04-11T20:02:53Z |
| format | Article |
| id | doaj.art-c6769d62f15c4a3cb692b7d48247b39d |
| institution | Directory Open Access Journal |
| issn | 1687-966X 1687-9678 |
| language | English |
| last_indexed | 2024-04-11T20:02:53Z |
| publishDate | 2021-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj.art-c6769d62f15c4a3cb692b7d48247b39d2022-12-22T04:05:32ZengHindawi LimitedStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/68649886864988Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage TemperaturesValentin Schrodi0Claudia Neunaber1Katrin Bundkirchen2Weikang Ye3Zhida Jiang4Maximilian Petri5Christian Krettek6Sandra Noack7Trauma Department, Hannover Medical School, 30625 Hannover, GermanyTrauma Department, Hannover Medical School, 30625 Hannover, GermanyTrauma Department, Hannover Medical School, 30625 Hannover, GermanyTrauma Department, Hannover Medical School, 30625 Hannover, GermanyTrauma Department, Hannover Medical School, 30625 Hannover, GermanyTrauma Department, Hannover Medical School, 30625 Hannover, GermanyTrauma Department, Hannover Medical School, 30625 Hannover, GermanyTrauma Department, Hannover Medical School, 30625 Hannover, GermanyMesenchymal stem cells play an important role in regenerative medicine due to their capability of self-renewal and multipotent differentiation. For research or clinical application, bone marrow aspirates are harvested during elective surgeries to isolate MSCs. If an immediate purification of the MSCs is not possible, the bone marrow must be stored. Therefore, the aim of this study was to investigate possible differences of stem cell characteristics regarding the self-renewal capability, the adipogenic, chondrogenic, and osteogenic differentiation, and the expression of surface antigens after different storage conditions of the bone marrow aspirates. Three groups were analysed: the first group was purified immediately after harvesting, the other two groups were processed after they were stored 18 to 24 hours at 22°C (room temperature) or at 4°C. Comparisons between the groups were performed using the Kruskal-Wallis test for nonparametric data. The final results showed no significant difference between the different storage conditions. Therefore, storage of bone marrow aspirates for 18 to 24 hours at room temperature or 4°C is possible without loss of stem cell characteristics.http://dx.doi.org/10.1155/2021/6864988 |
| spellingShingle | Valentin Schrodi Claudia Neunaber Katrin Bundkirchen Weikang Ye Zhida Jiang Maximilian Petri Christian Krettek Sandra Noack Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage Temperatures Stem Cells International |
| title | Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage Temperatures |
| title_full | Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage Temperatures |
| title_fullStr | Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage Temperatures |
| title_full_unstemmed | Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage Temperatures |
| title_short | Characteristics of Mesenchymal Stem Cells Are Independent of Bone Marrow Storage Temperatures |
| title_sort | characteristics of mesenchymal stem cells are independent of bone marrow storage temperatures |
| url | http://dx.doi.org/10.1155/2021/6864988 |
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