Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
Pal Patel, Krupa Kansara, Ragini Singh, Ritesh K Shukla, Sanjay Singh, Alok Dhawan, Ashutosh Kumar Division of Biological and Life Sciences (Formerly Institute of Life Sciences), School of Arts and Sciences, Ahmedabad University, Ahmedabad, Gujarat, India Abstract: Overproduction of free radicals...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-03-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/cellular-internalization-and-antioxidant-activity-of-cerium-oxide-nano-peer-reviewed-article-IJN |
| _version_ | 1818421164828000256 |
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| author | Patel P Kansara K Singh R Shukla RK Singh S Dhawan A Kumar A |
| author_facet | Patel P Kansara K Singh R Shukla RK Singh S Dhawan A Kumar A |
| author_sort | Patel P |
| collection | DOAJ |
| description | Pal Patel, Krupa Kansara, Ragini Singh, Ritesh K Shukla, Sanjay Singh, Alok Dhawan, Ashutosh Kumar Division of Biological and Life Sciences (Formerly Institute of Life Sciences), School of Arts and Sciences, Ahmedabad University, Ahmedabad, Gujarat, India Abstract: Overproduction of free radicals contributes to oxidative stress and inflammation leading to various disease conditions. Cerium oxide nanoparticles (nanoceria) have been shown to scavenge free radicals and have the potential for being used as a therapeutic agent in disease conditions. Therefore, in the present study, human monocytic leukemia cells (THP-1) were used as a model to evaluate the uptake and free radical scavenging activity of nanoceria. Our data showed a significant (P<0.05) increase in the internalization of nanoceria in a concentration-dependent (10–100 µg/mL) manner in THP-1 cells. Although no cytotoxicity was observed at these concentrations, nanoceria significantly (P<0.05) reduced the amount of reactive oxygen species. This was evident by a significant (P<0.05) decrease in the 2,7-dichlorofluorescein diacetate fluorescence observed in flow cytometry and fluorescence microscopy. The present study shows that nanoceria have therapeutic potential in diseases such as cancer. Keywords: nanoceria, reactive oxygen species, inflammation, free radicals, antioxidant |
| first_indexed | 2024-12-14T13:06:01Z |
| format | Article |
| id | doaj.art-c676a65906bc4e49869e99d4a0bab510 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T13:06:01Z |
| publishDate | 2018-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-c676a65906bc4e49869e99d4a0bab5102022-12-21T23:00:19ZengDove Medical PressInternational Journal of Nanomedicine1178-20132018-03-01Volume 13394137257Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cellsPatel PKansara KSingh RShukla RKSingh SDhawan AKumar APal Patel, Krupa Kansara, Ragini Singh, Ritesh K Shukla, Sanjay Singh, Alok Dhawan, Ashutosh Kumar Division of Biological and Life Sciences (Formerly Institute of Life Sciences), School of Arts and Sciences, Ahmedabad University, Ahmedabad, Gujarat, India Abstract: Overproduction of free radicals contributes to oxidative stress and inflammation leading to various disease conditions. Cerium oxide nanoparticles (nanoceria) have been shown to scavenge free radicals and have the potential for being used as a therapeutic agent in disease conditions. Therefore, in the present study, human monocytic leukemia cells (THP-1) were used as a model to evaluate the uptake and free radical scavenging activity of nanoceria. Our data showed a significant (P<0.05) increase in the internalization of nanoceria in a concentration-dependent (10–100 µg/mL) manner in THP-1 cells. Although no cytotoxicity was observed at these concentrations, nanoceria significantly (P<0.05) reduced the amount of reactive oxygen species. This was evident by a significant (P<0.05) decrease in the 2,7-dichlorofluorescein diacetate fluorescence observed in flow cytometry and fluorescence microscopy. The present study shows that nanoceria have therapeutic potential in diseases such as cancer. Keywords: nanoceria, reactive oxygen species, inflammation, free radicals, antioxidanthttps://www.dovepress.com/cellular-internalization-and-antioxidant-activity-of-cerium-oxide-nano-peer-reviewed-article-IJNNanoceriaReactive Oxygen SpeciesInflammationFree radicalsAntioxidant. |
| spellingShingle | Patel P Kansara K Singh R Shukla RK Singh S Dhawan A Kumar A Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells International Journal of Nanomedicine Nanoceria Reactive Oxygen Species Inflammation Free radicals Antioxidant. |
| title | Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells |
| title_full | Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells |
| title_fullStr | Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells |
| title_full_unstemmed | Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells |
| title_short | Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells |
| title_sort | cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells |
| topic | Nanoceria Reactive Oxygen Species Inflammation Free radicals Antioxidant. |
| url | https://www.dovepress.com/cellular-internalization-and-antioxidant-activity-of-cerium-oxide-nano-peer-reviewed-article-IJN |
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