Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in Vitro
The deciduous tree Terminalia bellirica found in Southeast Asia is extensively used in traditional Indian Ayurvedic medicine for the treatment of hypertension, rheumatism, and diabetes. The anti-atherogenic effect of Terminalia bellirica fruit has not been fully elucidated. Here, we investigated the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-06-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/5/2/20 |
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| author | Miori Tanaka Yoshimi Kishimoto Emi Saita Norie Suzuki-Sugihara Tomoyasu Kamiya Chie Taguchi Kaoruko Iida Kazuo Kondo |
| author_facet | Miori Tanaka Yoshimi Kishimoto Emi Saita Norie Suzuki-Sugihara Tomoyasu Kamiya Chie Taguchi Kaoruko Iida Kazuo Kondo |
| author_sort | Miori Tanaka |
| collection | DOAJ |
| description | The deciduous tree Terminalia bellirica found in Southeast Asia is extensively used in traditional Indian Ayurvedic medicine for the treatment of hypertension, rheumatism, and diabetes. The anti-atherogenic effect of Terminalia bellirica fruit has not been fully elucidated. Here, we investigated the effect of Terminalia bellirica extract (TBE) on low-density lipoprotein (LDL) oxidation and inflammation in macrophages. TBE showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (EC50: 7.2 ± 1.2 μg/mL) and 15-lipoxygenase inhibitory activity. TBE also significantly inhibited free radical-induced LDL oxidation compared to the solvent control in vitro. In THP-1 macrophages, TBE treatment resulted in significant decreases of the mRNA expression of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and lectin-like oxidized LDL receptor-1 (LOX-1). TBE also reduced matrix metalloproteinase (MMP)-9 secretion and intracellular reactive oxygen species (ROS) production in THP-1 macrophages. These results show that TBE has the inhibitory effects on LDL oxidation and macrophage inflammatory response in vitro, suggesting that its in vivo use might inhibit atherosclerosis plaque progression. |
| first_indexed | 2024-03-12T11:04:45Z |
| format | Article |
| id | doaj.art-c678032af81547989d2b68c346b81ef7 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T11:04:45Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-c678032af81547989d2b68c346b81ef72023-09-02T04:08:54ZengMDPI AGAntioxidants2076-39212016-06-01522010.3390/antiox5020020antiox5020020Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in VitroMiori Tanaka0Yoshimi Kishimoto1Emi Saita2Norie Suzuki-Sugihara3Tomoyasu Kamiya4Chie Taguchi5Kaoruko Iida6Kazuo Kondo7Department of Food and Nutritional Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, JapanEndowed Research Department “Food for Health”, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, JapanEndowed Research Department “Food for Health”, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, JapanDepartment of Food and Nutritional Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, JapanResearch and Development Division, Toyo Shinyaku Co Ltd., 7-28 Yayoigaoka, Tosu-shi, Saga 841-0005, JapanEndowed Research Department “Food for Health”, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, JapanDepartment of Food and Nutritional Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, JapanEndowed Research Department “Food for Health”, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, JapanThe deciduous tree Terminalia bellirica found in Southeast Asia is extensively used in traditional Indian Ayurvedic medicine for the treatment of hypertension, rheumatism, and diabetes. The anti-atherogenic effect of Terminalia bellirica fruit has not been fully elucidated. Here, we investigated the effect of Terminalia bellirica extract (TBE) on low-density lipoprotein (LDL) oxidation and inflammation in macrophages. TBE showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (EC50: 7.2 ± 1.2 μg/mL) and 15-lipoxygenase inhibitory activity. TBE also significantly inhibited free radical-induced LDL oxidation compared to the solvent control in vitro. In THP-1 macrophages, TBE treatment resulted in significant decreases of the mRNA expression of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and lectin-like oxidized LDL receptor-1 (LOX-1). TBE also reduced matrix metalloproteinase (MMP)-9 secretion and intracellular reactive oxygen species (ROS) production in THP-1 macrophages. These results show that TBE has the inhibitory effects on LDL oxidation and macrophage inflammatory response in vitro, suggesting that its in vivo use might inhibit atherosclerosis plaque progression.http://www.mdpi.com/2076-3921/5/2/20Terminalia belliricapolyphenollipid oxidationmacrophageinflammationatherosclerosis |
| spellingShingle | Miori Tanaka Yoshimi Kishimoto Emi Saita Norie Suzuki-Sugihara Tomoyasu Kamiya Chie Taguchi Kaoruko Iida Kazuo Kondo Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in Vitro Antioxidants Terminalia bellirica polyphenol lipid oxidation macrophage inflammation atherosclerosis |
| title | Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in Vitro |
| title_full | Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in Vitro |
| title_fullStr | Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in Vitro |
| title_full_unstemmed | Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in Vitro |
| title_short | Terminalia bellirica Extract Inhibits Low-Density Lipoprotein Oxidation and Macrophage Inflammatory Response in Vitro |
| title_sort | terminalia bellirica extract inhibits low density lipoprotein oxidation and macrophage inflammatory response in vitro |
| topic | Terminalia bellirica polyphenol lipid oxidation macrophage inflammation atherosclerosis |
| url | http://www.mdpi.com/2076-3921/5/2/20 |
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