Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk
IntroductionA healthy pregnancy requires successful blastocyst implantation into an adequately prepared or ‘receptive’ endometrium. Decidualization of uterine endometrial stromal fibroblast cells (hESF) is critical for the establishment of a healthy pregnancy. microRNAs (miRs) are critical regulator...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1149786/full |
| _version_ | 1797869573420089344 |
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| author | Ellen Menkhorst Ellen Menkhorst Teresa So Teresa So Kate Rainczuk Siena Barton Siena Barton Wei Zhou Wei Zhou Tracey Edgell Evdokia Dimitriadis Evdokia Dimitriadis Evdokia Dimitriadis |
| author_facet | Ellen Menkhorst Ellen Menkhorst Teresa So Teresa So Kate Rainczuk Siena Barton Siena Barton Wei Zhou Wei Zhou Tracey Edgell Evdokia Dimitriadis Evdokia Dimitriadis Evdokia Dimitriadis |
| author_sort | Ellen Menkhorst |
| collection | DOAJ |
| description | IntroductionA healthy pregnancy requires successful blastocyst implantation into an adequately prepared or ‘receptive’ endometrium. Decidualization of uterine endometrial stromal fibroblast cells (hESF) is critical for the establishment of a healthy pregnancy. microRNAs (miRs) are critical regulators of cellular function that can be released by a donor cell to influence the physiological state of recipient cells. We aimed to determine how decidualization affects hESF miR release and investigated the function of one decidualization regulated miR, miR-19b-3p, previously shown to be associated with recurrent pregnancy loss.MethodmiR release by hESF was determined by miR microarray on culture media from hESF decidualized in vitro for 3 and 14 days by treatment with oestradiol and medroxyprogesterone acetate. Cellular and whole endometrial/decidual tissue miR expression was quantified by qPCR and localized by in situ hybridization. The function of miR-19b-3p in HTR8/Svneo trophoblast cells was investigated using real time cell analysis (xCELLigence) and gene expression qPCR.ResultsFrom our miR screen we found that essentially all hESF miR release was reduced following in vitro decidualization, significantly so for miR-17-5p, miR-21-3p, miR-34c-3p, miR-106b-5p, miR-138-5p, miR-296-5p, miR-323a-3p, miR-342-3p, miR-491-5p, miR-503-5p and miR-542-5p. qPCR demonstrated that miR-19b-3p, 181a-2-3p and miR-409-5p likewise showed a significant reduction in culture media following decidualization but no change was found in cellular miR expression following decidualization. In situ hybridization localized miR-19b-3p to epithelial and stromal cells in the endometrium and qPCR identified that miR-19b-3p was significantly elevated in the cycling endometrium of patients with a history of early pregnancy loss compared to normally fertile controls. Functionally, overexpression of miR-19b-3p significantly reduced HTR8/Svneo trophoblast proliferation and increased HOXA9 expression.DiscussionOur data demonstrates that decidualization represses miR release by hESFs and overexpression of miR-19b-3p was found in endometrial tissue from patients with a history of early pregnancy loss. miR-19b-3p impaired HTR8/Svneo proliferation implying a role in trophoblast function. Overall we speculate that miR release by hESF may regulate other cell types within the decidua and that appropriate release of miRs by decidualized hESF is essential for healthy implantation and placentation. |
| first_indexed | 2024-04-10T00:13:43Z |
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| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-10T00:13:43Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-c67a1a3adcf942168c71d98dd832fc8a2023-03-16T07:14:11ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-03-011410.3389/fendo.2023.11497861149786Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talkEllen Menkhorst0Ellen Menkhorst1Teresa So2Teresa So3Kate Rainczuk4Siena Barton5Siena Barton6Wei Zhou7Wei Zhou8Tracey Edgell9Evdokia Dimitriadis10Evdokia Dimitriadis11Evdokia Dimitriadis12Embryo Implantation Laboratory, Department of Obstetrics and Gynecology, The University of Melbourne, Parkville, VIC, AustraliaGynecology Research Centre, The Royal Women’s Hospital, Parkville, VIC, AustraliaEmbryo Implantation Laboratory, Department of Obstetrics and Gynecology, The University of Melbourne, Parkville, VIC, AustraliaGynecology Research Centre, The Royal Women’s Hospital, Parkville, VIC, AustraliaCentre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, AustraliaEmbryo Implantation Laboratory, Department of Obstetrics and Gynecology, The University of Melbourne, Parkville, VIC, AustraliaGynecology Research Centre, The Royal Women’s Hospital, Parkville, VIC, AustraliaEmbryo Implantation Laboratory, Department of Obstetrics and Gynecology, The University of Melbourne, Parkville, VIC, AustraliaGynecology Research Centre, The Royal Women’s Hospital, Parkville, VIC, AustraliaCentre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, AustraliaEmbryo Implantation Laboratory, Department of Obstetrics and Gynecology, The University of Melbourne, Parkville, VIC, AustraliaGynecology Research Centre, The Royal Women’s Hospital, Parkville, VIC, AustraliaCentre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, AustraliaIntroductionA healthy pregnancy requires successful blastocyst implantation into an adequately prepared or ‘receptive’ endometrium. Decidualization of uterine endometrial stromal fibroblast cells (hESF) is critical for the establishment of a healthy pregnancy. microRNAs (miRs) are critical regulators of cellular function that can be released by a donor cell to influence the physiological state of recipient cells. We aimed to determine how decidualization affects hESF miR release and investigated the function of one decidualization regulated miR, miR-19b-3p, previously shown to be associated with recurrent pregnancy loss.MethodmiR release by hESF was determined by miR microarray on culture media from hESF decidualized in vitro for 3 and 14 days by treatment with oestradiol and medroxyprogesterone acetate. Cellular and whole endometrial/decidual tissue miR expression was quantified by qPCR and localized by in situ hybridization. The function of miR-19b-3p in HTR8/Svneo trophoblast cells was investigated using real time cell analysis (xCELLigence) and gene expression qPCR.ResultsFrom our miR screen we found that essentially all hESF miR release was reduced following in vitro decidualization, significantly so for miR-17-5p, miR-21-3p, miR-34c-3p, miR-106b-5p, miR-138-5p, miR-296-5p, miR-323a-3p, miR-342-3p, miR-491-5p, miR-503-5p and miR-542-5p. qPCR demonstrated that miR-19b-3p, 181a-2-3p and miR-409-5p likewise showed a significant reduction in culture media following decidualization but no change was found in cellular miR expression following decidualization. In situ hybridization localized miR-19b-3p to epithelial and stromal cells in the endometrium and qPCR identified that miR-19b-3p was significantly elevated in the cycling endometrium of patients with a history of early pregnancy loss compared to normally fertile controls. Functionally, overexpression of miR-19b-3p significantly reduced HTR8/Svneo trophoblast proliferation and increased HOXA9 expression.DiscussionOur data demonstrates that decidualization represses miR release by hESFs and overexpression of miR-19b-3p was found in endometrial tissue from patients with a history of early pregnancy loss. miR-19b-3p impaired HTR8/Svneo proliferation implying a role in trophoblast function. Overall we speculate that miR release by hESF may regulate other cell types within the decidua and that appropriate release of miRs by decidualized hESF is essential for healthy implantation and placentation.https://www.frontiersin.org/articles/10.3389/fendo.2023.1149786/fulldeciduadecidualizationmicroRNA releasemiR-19b-3ptrophoblastearly pregnancy loss |
| spellingShingle | Ellen Menkhorst Ellen Menkhorst Teresa So Teresa So Kate Rainczuk Siena Barton Siena Barton Wei Zhou Wei Zhou Tracey Edgell Evdokia Dimitriadis Evdokia Dimitriadis Evdokia Dimitriadis Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk Frontiers in Endocrinology decidua decidualization microRNA release miR-19b-3p trophoblast early pregnancy loss |
| title | Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk |
| title_full | Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk |
| title_fullStr | Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk |
| title_full_unstemmed | Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk |
| title_short | Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk |
| title_sort | endometrial stromal cell mir 19b 3p release is reduced during decidualization implying a role in decidual trophoblast cross talk |
| topic | decidua decidualization microRNA release miR-19b-3p trophoblast early pregnancy loss |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1149786/full |
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