Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity
Innovative and highly performing smart voltammetric immunosensors for rapid and effective serological tests aimed at the determination of SARS-CoV-2 antibodies were developed and validated in human serum matrix. Two immunosensors were developed for the determination of immunoglobulins directed again...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/14/5463 |
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| author | Simone Fortunati Marco Giannetto Chiara Giliberti Angelo Bolchi Davide Ferrari Massimo Locatelli Valentina Bianchi Andrea Boni Ilaria De Munari Maria Careri |
| author_facet | Simone Fortunati Marco Giannetto Chiara Giliberti Angelo Bolchi Davide Ferrari Massimo Locatelli Valentina Bianchi Andrea Boni Ilaria De Munari Maria Careri |
| author_sort | Simone Fortunati |
| collection | DOAJ |
| description | Innovative and highly performing smart voltammetric immunosensors for rapid and effective serological tests aimed at the determination of SARS-CoV-2 antibodies were developed and validated in human serum matrix. Two immunosensors were developed for the determination of immunoglobulins directed against either the nucleocapsid or the spike viral antigen proteins. The immunosensors were realized using disposable screen-printed electrodes modified with nanostructured materials for the immobilization of the antigens. Fast quantitative detection was achieved, with analysis duration being around 1 h. Signal readout was carried out through a smart, compact and battery-powered potentiostat, based on a Wi-Fi protocol and devised for the Internet of Things (IoT) paradigm. This device is used for the acquisition, storage and sharing of clinical data. Outstanding immunosensors’ sensitivity, specificity and accuracy (100%) were assessed, according to the diagnostic guidelines for epidemiological data. The overall performance of the sensing devices, combined with the portability of the IoT-based device, enables their suitability as a high-throughput diagnostic tool. Both of the immunosensors were validated using clinical human serum specimens from SARS-CoV-2 infected patients, provided by IRCCS Ospedale San Raffaele. |
| first_indexed | 2024-03-09T13:02:06Z |
| format | Article |
| id | doaj.art-c67ae2005c9f47ff9ae5da6cb8f0830c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T13:02:06Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-c67ae2005c9f47ff9ae5da6cb8f0830c2023-11-30T21:53:00ZengMDPI AGSensors1424-82202022-07-012214546310.3390/s22145463Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 ImmunitySimone Fortunati0Marco Giannetto1Chiara Giliberti2Angelo Bolchi3Davide Ferrari4Massimo Locatelli5Valentina Bianchi6Andrea Boni7Ilaria De Munari8Maria Careri9Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyDipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyDipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyDipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyDipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyIRCCS Ospedale San Raffaele, Via Olgettina 60, 20132 Milano, ItalyDipartimento di Ingegneria e Architettura, Università di Parma, Parco Area delle Scienze 181/A, 43124 Parma, ItalyDipartimento di Ingegneria e Architettura, Università di Parma, Parco Area delle Scienze 181/A, 43124 Parma, ItalyDipartimento di Ingegneria e Architettura, Università di Parma, Parco Area delle Scienze 181/A, 43124 Parma, ItalyDipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyInnovative and highly performing smart voltammetric immunosensors for rapid and effective serological tests aimed at the determination of SARS-CoV-2 antibodies were developed and validated in human serum matrix. Two immunosensors were developed for the determination of immunoglobulins directed against either the nucleocapsid or the spike viral antigen proteins. The immunosensors were realized using disposable screen-printed electrodes modified with nanostructured materials for the immobilization of the antigens. Fast quantitative detection was achieved, with analysis duration being around 1 h. Signal readout was carried out through a smart, compact and battery-powered potentiostat, based on a Wi-Fi protocol and devised for the Internet of Things (IoT) paradigm. This device is used for the acquisition, storage and sharing of clinical data. Outstanding immunosensors’ sensitivity, specificity and accuracy (100%) were assessed, according to the diagnostic guidelines for epidemiological data. The overall performance of the sensing devices, combined with the portability of the IoT-based device, enables their suitability as a high-throughput diagnostic tool. Both of the immunosensors were validated using clinical human serum specimens from SARS-CoV-2 infected patients, provided by IRCCS Ospedale San Raffaele.https://www.mdpi.com/1424-8220/22/14/5463SARS-CoV-2COVID-19serological testimmunosensorpoint-of-care testingIoT-Wi-Fi |
| spellingShingle | Simone Fortunati Marco Giannetto Chiara Giliberti Angelo Bolchi Davide Ferrari Massimo Locatelli Valentina Bianchi Andrea Boni Ilaria De Munari Maria Careri Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity Sensors SARS-CoV-2 COVID-19 serological test immunosensor point-of-care testing IoT-Wi-Fi |
| title | Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity |
| title_full | Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity |
| title_fullStr | Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity |
| title_full_unstemmed | Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity |
| title_short | Smart Immunosensors for Point-of-Care Serological Tests Aimed at Assessing Natural or Vaccine-Induced SARS-CoV-2 Immunity |
| title_sort | smart immunosensors for point of care serological tests aimed at assessing natural or vaccine induced sars cov 2 immunity |
| topic | SARS-CoV-2 COVID-19 serological test immunosensor point-of-care testing IoT-Wi-Fi |
| url | https://www.mdpi.com/1424-8220/22/14/5463 |
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