Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study
Background and Objectives: Breast cancer is currently the most commonly diagnosed neoplasm in women worldwide. There is evidence that human papillomavirus (HPV) infection may play a key role in breast cancer aggressiveness, but results are conflicting across studies. The aim of this study was to in...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-08-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4139 |
| _version_ | 1797688922307821568 |
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| author | Manoochehr Makvandi Mohammad Rashno Ebrahim Faghihloo Shahram Bagheri Saeed Hesam Habibollah Mirzaei Ali Ramezani |
| author_facet | Manoochehr Makvandi Mohammad Rashno Ebrahim Faghihloo Shahram Bagheri Saeed Hesam Habibollah Mirzaei Ali Ramezani |
| author_sort | Manoochehr Makvandi |
| collection | DOAJ |
| description |
Background and Objectives: Breast cancer is currently the most commonly diagnosed neoplasm in women worldwide. There is evidence that human papillomavirus (HPV) infection may play a key role in breast cancer aggressiveness, but results are conflicting across studies. The aim of this study was to investigate the presence of the HPV viral genome in benign and malignant breast tissue samples and its clinicopathological characteristics of cancer.
Materials and Methods: In this case-control study, 100 formalin-fixed paraffin-embedded (FFPE) of breast cancer and 100 blocks of non-cancerous breast tissue were selected as a control group from the pathology department of Imam Khomeini Hospital in Ahvaz from 2020-2022. The presence of HPV was detected using nested PCR including MY09/11 primers and sequencing were performed for virus genotyping.
Results: The present study enrolled 100 subjects each in two cancer and control groups with a mean age of 52.81±13.23 and 35.77±11.65, respectively. The risk of cancer in HPV-infected patients is almost 5 times higher than in HPV-negative individuals, it is not statistically significant (OR =4.99, 95% CI 0.35 to 72.15, p=0.238). The prevalence of HPV in the cancer and control groups was 7% and 1%, respectively and HPVs detected in two groups were of the HPV 16 genotype. Although the chance of ER and PR expression, lymphvascular involvement, perineural invasion, and higher tumor grade was higher in HPV-positive subjects than in HPV-negative subjects, this was not statistically significant (OR>1, p>0.05).
Conclusion: Based on studies reporting the existence of sequences of different high-risk HPV types (oncogenes) in breast cancer tissues, this study confirmed the hypothesis of a possible infectious cause in the development of breast cancer. So far, however, the results have been controversial and inconclusive. Further studies with large sample sizes are needed to demonstrate the link between HPV and breast cancer.
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| first_indexed | 2024-03-12T01:37:33Z |
| format | Article |
| id | doaj.art-c67bbe0d3e1845a6ad3e779bd0fcd78b |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-12T01:37:33Z |
| publishDate | 2023-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-c67bbe0d3e1845a6ad3e779bd0fcd78b2023-09-11T05:04:00ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-08-0115410.18502/ijm.v15i4.13513Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control studyManoochehr Makvandi0Mohammad Rashno1Ebrahim Faghihloo2Shahram Bagheri3Saeed Hesam4Habibollah Mirzaei5Ali Ramezani6Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pathology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Biostatistics and Epidemiology, Faculty of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Background and Objectives: Breast cancer is currently the most commonly diagnosed neoplasm in women worldwide. There is evidence that human papillomavirus (HPV) infection may play a key role in breast cancer aggressiveness, but results are conflicting across studies. The aim of this study was to investigate the presence of the HPV viral genome in benign and malignant breast tissue samples and its clinicopathological characteristics of cancer. Materials and Methods: In this case-control study, 100 formalin-fixed paraffin-embedded (FFPE) of breast cancer and 100 blocks of non-cancerous breast tissue were selected as a control group from the pathology department of Imam Khomeini Hospital in Ahvaz from 2020-2022. The presence of HPV was detected using nested PCR including MY09/11 primers and sequencing were performed for virus genotyping. Results: The present study enrolled 100 subjects each in two cancer and control groups with a mean age of 52.81±13.23 and 35.77±11.65, respectively. The risk of cancer in HPV-infected patients is almost 5 times higher than in HPV-negative individuals, it is not statistically significant (OR =4.99, 95% CI 0.35 to 72.15, p=0.238). The prevalence of HPV in the cancer and control groups was 7% and 1%, respectively and HPVs detected in two groups were of the HPV 16 genotype. Although the chance of ER and PR expression, lymphvascular involvement, perineural invasion, and higher tumor grade was higher in HPV-positive subjects than in HPV-negative subjects, this was not statistically significant (OR>1, p>0.05). Conclusion: Based on studies reporting the existence of sequences of different high-risk HPV types (oncogenes) in breast cancer tissues, this study confirmed the hypothesis of a possible infectious cause in the development of breast cancer. So far, however, the results have been controversial and inconclusive. Further studies with large sample sizes are needed to demonstrate the link between HPV and breast cancer. https://ijm.tums.ac.ir/index.php/ijm/article/view/4139Breast malignant tumor;Human papillomavirus;Prognostic factors;Nested polymerase chain reaction;Prevalence |
| spellingShingle | Manoochehr Makvandi Mohammad Rashno Ebrahim Faghihloo Shahram Bagheri Saeed Hesam Habibollah Mirzaei Ali Ramezani Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study Iranian Journal of Microbiology Breast malignant tumor; Human papillomavirus; Prognostic factors; Nested polymerase chain reaction; Prevalence |
| title | Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study |
| title_full | Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study |
| title_fullStr | Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study |
| title_full_unstemmed | Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study |
| title_short | Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study |
| title_sort | low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer a case control study |
| topic | Breast malignant tumor; Human papillomavirus; Prognostic factors; Nested polymerase chain reaction; Prevalence |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4139 |
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