Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i>
Recently, the transgenerational toxicity of nanoplastics has received increasing attention. <i>Caenorhabditis elegans</i> is a useful model to assess the transgenerational toxicity of different pollutants. In nematodes, the possibility of early-life exposure to sulfonate-modified polysty...
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MDPI AG
2023-06-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/6/511 |
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| author | Wenmiao He Aihua Gu Dayong Wang |
| author_facet | Wenmiao He Aihua Gu Dayong Wang |
| author_sort | Wenmiao He |
| collection | DOAJ |
| description | Recently, the transgenerational toxicity of nanoplastics has received increasing attention. <i>Caenorhabditis elegans</i> is a useful model to assess the transgenerational toxicity of different pollutants. In nematodes, the possibility of early-life exposure to sulfonate-modified polystyrene nanoparticle (PS-S NP) causing transgenerational toxicity and its underlying mechanisms were investigated. After exposure at the L1-larval stage, transgenerational inhibition in both locomotion behavior (body bend and head thrash) and reproductive capacity (number of offspring and fertilized egg number in uterus) was induced by 1–100 μg/L PS-S NP. Meanwhile, after exposure to 1–100 μg/L PS-S NP, the expression of germline <i>lag-2</i> encoding Notch ligand was increased not only at the parental generation (P0-G) but also in the offspring, and the transgenerational toxicity was inhibited by the germline RNA interference (RNAi) of <i>lag-2</i>. During the transgenerational toxicity formation, the parental LAG-2 activated the corresponding Notch receptor GLP-1 in the offspring, and transgenerational toxicity was also suppressed by <i>glp-1</i> RNAi. GLP-1 functioned in the germline and the neurons to mediate the PS-S NP toxicity. In PS-S NP-exposed nematodes, germline GLP-1 activated the insulin peptides of INS-39, INS-3, and DAF-28, and neuronal GLP-1 inhibited the DAF-7, DBL-1, and GLB-10. Therefore, the exposure risk in inducing transgenerational toxicity through PS-S NP was suggested, and this transgenerational toxicity was mediated by the activation of germline Notch signal in organisms. |
| first_indexed | 2024-03-11T01:52:26Z |
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| id | doaj.art-c67d5976abc44a288958fa69d349c776 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-11T01:52:26Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Toxics |
| spelling | doaj.art-c67d5976abc44a288958fa69d349c7762023-11-18T12:54:37ZengMDPI AGToxics2305-63042023-06-0111651110.3390/toxics11060511Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i>Wenmiao He0Aihua Gu1Dayong Wang2Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing 210009, ChinaSchool of Public Health, Nanjing Medical University, Nanjing 211166, ChinaKey Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing 210009, ChinaRecently, the transgenerational toxicity of nanoplastics has received increasing attention. <i>Caenorhabditis elegans</i> is a useful model to assess the transgenerational toxicity of different pollutants. In nematodes, the possibility of early-life exposure to sulfonate-modified polystyrene nanoparticle (PS-S NP) causing transgenerational toxicity and its underlying mechanisms were investigated. After exposure at the L1-larval stage, transgenerational inhibition in both locomotion behavior (body bend and head thrash) and reproductive capacity (number of offspring and fertilized egg number in uterus) was induced by 1–100 μg/L PS-S NP. Meanwhile, after exposure to 1–100 μg/L PS-S NP, the expression of germline <i>lag-2</i> encoding Notch ligand was increased not only at the parental generation (P0-G) but also in the offspring, and the transgenerational toxicity was inhibited by the germline RNA interference (RNAi) of <i>lag-2</i>. During the transgenerational toxicity formation, the parental LAG-2 activated the corresponding Notch receptor GLP-1 in the offspring, and transgenerational toxicity was also suppressed by <i>glp-1</i> RNAi. GLP-1 functioned in the germline and the neurons to mediate the PS-S NP toxicity. In PS-S NP-exposed nematodes, germline GLP-1 activated the insulin peptides of INS-39, INS-3, and DAF-28, and neuronal GLP-1 inhibited the DAF-7, DBL-1, and GLB-10. Therefore, the exposure risk in inducing transgenerational toxicity through PS-S NP was suggested, and this transgenerational toxicity was mediated by the activation of germline Notch signal in organisms.https://www.mdpi.com/2305-6304/11/6/511<i>C. elegans</i>transgenerational toxicitynanoplasticNotch signal |
| spellingShingle | Wenmiao He Aihua Gu Dayong Wang Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i> Toxics <i>C. elegans</i> transgenerational toxicity nanoplastic Notch signal |
| title | Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i> |
| title_full | Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i> |
| title_fullStr | Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i> |
| title_short | Sulfonate-Modified Polystyrene Nanoparticle at Precited Environmental Concentrations Induces Transgenerational Toxicity Associated with Increase in Germline Notch Signal of <i>Caenorhabditis elegans</i> |
| title_sort | sulfonate modified polystyrene nanoparticle at precited environmental concentrations induces transgenerational toxicity associated with increase in germline notch signal of i caenorhabditis elegans i |
| topic | <i>C. elegans</i> transgenerational toxicity nanoplastic Notch signal |
| url | https://www.mdpi.com/2305-6304/11/6/511 |
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