FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2
Food irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2-Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain...
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| Format: | Article |
| Language: | English |
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Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)
2019-02-01
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| Series: | Brazilian Journal of Radiation Sciences |
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| Online Access: | https://bjrs.org.br/revista/index.php/REVISTA/article/view/684 |
| _version_ | 1811234604180307968 |
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| author | Angélica Bueno Barbezan |
| author_facet | Angélica Bueno Barbezan |
| author_sort | Angélica Bueno Barbezan |
| collection | DOAJ |
| description | Food irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2-Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain fatty acids (Triglycerides) when irradiated. The acids analyzed in this study are palmitic and stearic, which when irradiated form 2-Dodecylcyclobutanones (2-dDCB) and 2-Tetradecylcyclobutanone (2-tDCB). Part of the 2-ACBs ingested is excreted through feces and part is deposited in adipose tissues. In vitro studies so far have been only in colon cells. The work used a human hepatoma cell line (HepG2) since the accumulation of fat in this organ is quite common. Micronucleus test was selected to evaluate possible genotoxic effects of 2-dDCB and 2-tDCB compounds when exposed to high concentrations (447, 1422 and 2235 μM) for 4 and 24 hours. Tests were performed in quadriplicates using flow cytometric analysis. None detectable genotoxic damage was observed after 4 hours of exposure to the compounds, and cytotoxic effects were only significant at the highest concentration (2235 μM) of 2-dDCB. After 24 hours of exposure, slight genotoxic damage was observed at all concentrations evaluated, and cytotoxic effects were only present when exposed to compound 2-tDCB. Although there is a genotoxic and cytotoxic effect in some of the situations tested, the two compounds predominantly induced proliferation reduction effects of this hepatic tumor cell line. |
| first_indexed | 2024-04-12T11:39:00Z |
| format | Article |
| id | doaj.art-c680facf446d4560955567c351f06565 |
| institution | Directory Open Access Journal |
| issn | 2319-0612 |
| language | English |
| last_indexed | 2024-04-12T11:39:00Z |
| publishDate | 2019-02-01 |
| publisher | Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR) |
| record_format | Article |
| series | Brazilian Journal of Radiation Sciences |
| spelling | doaj.art-c680facf446d4560955567c351f065652022-12-22T03:34:44ZengBrazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)Brazilian Journal of Radiation Sciences2319-06122019-02-0172A10.15392/bjrs.v7i2A.684FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2Angélica Bueno BarbezanFood irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2-Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain fatty acids (Triglycerides) when irradiated. The acids analyzed in this study are palmitic and stearic, which when irradiated form 2-Dodecylcyclobutanones (2-dDCB) and 2-Tetradecylcyclobutanone (2-tDCB). Part of the 2-ACBs ingested is excreted through feces and part is deposited in adipose tissues. In vitro studies so far have been only in colon cells. The work used a human hepatoma cell line (HepG2) since the accumulation of fat in this organ is quite common. Micronucleus test was selected to evaluate possible genotoxic effects of 2-dDCB and 2-tDCB compounds when exposed to high concentrations (447, 1422 and 2235 μM) for 4 and 24 hours. Tests were performed in quadriplicates using flow cytometric analysis. None detectable genotoxic damage was observed after 4 hours of exposure to the compounds, and cytotoxic effects were only significant at the highest concentration (2235 μM) of 2-dDCB. After 24 hours of exposure, slight genotoxic damage was observed at all concentrations evaluated, and cytotoxic effects were only present when exposed to compound 2-tDCB. Although there is a genotoxic and cytotoxic effect in some of the situations tested, the two compounds predominantly induced proliferation reduction effects of this hepatic tumor cell line.https://bjrs.org.br/revista/index.php/REVISTA/article/view/684flow cytometry2-ACBsfood irradiation |
| spellingShingle | Angélica Bueno Barbezan FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2 Brazilian Journal of Radiation Sciences flow cytometry 2-ACBs food irradiation |
| title | FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2 |
| title_full | FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2 |
| title_fullStr | FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2 |
| title_full_unstemmed | FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2 |
| title_short | FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2 |
| title_sort | flow cytometry based micronucleus assay for evaluation of genotoxic potential of 2 acbs in hepatic cells hepg2 |
| topic | flow cytometry 2-ACBs food irradiation |
| url | https://bjrs.org.br/revista/index.php/REVISTA/article/view/684 |
| work_keys_str_mv | AT angelicabuenobarbezan flowcytometrybasedmicronucleusassayforevaluationofgenotoxicpotentialof2acbsinhepaticcellshepg2 |