The sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells
Abstract Background Marek’s disease (MD) is a highly contagious lymphoproliferative disease of chickens caused by an alphaherpesvirus, Marek’s disease virus (MDV). MD is presently controlled by systematic vaccination of animals, which protects efficiently against the development of clinical disease....
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03247-y |
| _version_ | 1828370745121570816 |
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| author | Frédérick Bussy Sylvie Rémy Matthieu Le Goff Pi Nyvall Collén Laëtitia Trapp-Fragnet |
| author_facet | Frédérick Bussy Sylvie Rémy Matthieu Le Goff Pi Nyvall Collén Laëtitia Trapp-Fragnet |
| author_sort | Frédérick Bussy |
| collection | DOAJ |
| description | Abstract Background Marek’s disease (MD) is a highly contagious lymphoproliferative disease of chickens caused by an alphaherpesvirus, Marek’s disease virus (MDV). MD is presently controlled by systematic vaccination of animals, which protects efficiently against the development of clinical disease. However, MDV vaccines do not prevent the multiplication and spread of MDV field strains and may favor the emergence of strains with increased virulence. Therefore, MDV persists to be a major problem for the poultry industry and the development of new alternative strategies to control MDV is needed. Seaweed extracts have previously been shown to exert immunomodulatory and antiviral activities, especially against herpesviruses. The objective of the present study was to explore the effect of Ulva armoricana extracts on MDV infection in vitro. Results We could demonstrate that the ulvan extract as well as its vitamin-enriched formulation reduce the viral load by about 80% at 24 h post-infection in infected chicken fibroblasts at concentrations that are innocuous for the cells. We also observed a substantial decrease in MDV plaque size suggesting that ulvans impede MDV cell-to-cell spread in vitro. Moreover, we showed that ulvan extract could promote MDV reactivation in lymphoid cells. Conclusions Our data provide the first evidence that the use of the ulvan extract could be a good alternative to limit MDV infection in poultry. |
| first_indexed | 2024-04-14T06:41:25Z |
| format | Article |
| id | doaj.art-c68413d2c0eb432199a20ea740ce967f |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-14T06:41:25Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-c68413d2c0eb432199a20ea740ce967f2022-12-22T02:07:19ZengBMCBMC Veterinary Research1746-61482022-04-011811910.1186/s12917-022-03247-yThe sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cellsFrédérick Bussy0Sylvie Rémy1Matthieu Le Goff2Pi Nyvall Collén3Laëtitia Trapp-Fragnet4Amadeite SASINRAE, Université de ToursAmadeite SASAmadeite SASINRAE, Université de ToursAbstract Background Marek’s disease (MD) is a highly contagious lymphoproliferative disease of chickens caused by an alphaherpesvirus, Marek’s disease virus (MDV). MD is presently controlled by systematic vaccination of animals, which protects efficiently against the development of clinical disease. However, MDV vaccines do not prevent the multiplication and spread of MDV field strains and may favor the emergence of strains with increased virulence. Therefore, MDV persists to be a major problem for the poultry industry and the development of new alternative strategies to control MDV is needed. Seaweed extracts have previously been shown to exert immunomodulatory and antiviral activities, especially against herpesviruses. The objective of the present study was to explore the effect of Ulva armoricana extracts on MDV infection in vitro. Results We could demonstrate that the ulvan extract as well as its vitamin-enriched formulation reduce the viral load by about 80% at 24 h post-infection in infected chicken fibroblasts at concentrations that are innocuous for the cells. We also observed a substantial decrease in MDV plaque size suggesting that ulvans impede MDV cell-to-cell spread in vitro. Moreover, we showed that ulvan extract could promote MDV reactivation in lymphoid cells. Conclusions Our data provide the first evidence that the use of the ulvan extract could be a good alternative to limit MDV infection in poultry.https://doi.org/10.1186/s12917-022-03247-yMarek’s disease virusSeaweedUlva armoricanaSearup®CytotoxicityViral replication |
| spellingShingle | Frédérick Bussy Sylvie Rémy Matthieu Le Goff Pi Nyvall Collén Laëtitia Trapp-Fragnet The sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells BMC Veterinary Research Marek’s disease virus Seaweed Ulva armoricana Searup® Cytotoxicity Viral replication |
| title | The sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells |
| title_full | The sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells |
| title_fullStr | The sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells |
| title_full_unstemmed | The sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells |
| title_short | The sulphated polysaccharides extract ulvans from Ulva armoricana limits Marek’s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells |
| title_sort | sulphated polysaccharides extract ulvans from ulva armoricana limits marek s disease virus dissemination in vitro and promotes viral reactivation in lymphoid cells |
| topic | Marek’s disease virus Seaweed Ulva armoricana Searup® Cytotoxicity Viral replication |
| url | https://doi.org/10.1186/s12917-022-03247-y |
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