Capture and light-induced release of antibiotics by an azo dye polymer
Abstract The isomerisation of azo dyes can induce conformational changes which have potential applications in medicine and environmental protection. We developed an agar diffusion assay to test the capture and release of biologically active molecules from an azo electro-optic polymer, Poly (Disperse...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-60245-6 |
| _version_ | 1818420364190941184 |
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| author | Stephen Atkins Alysa Chueh Taylor Barwell Jean-Michel Nunzi Laurent Seroude |
| author_facet | Stephen Atkins Alysa Chueh Taylor Barwell Jean-Michel Nunzi Laurent Seroude |
| author_sort | Stephen Atkins |
| collection | DOAJ |
| description | Abstract The isomerisation of azo dyes can induce conformational changes which have potential applications in medicine and environmental protection. We developed an agar diffusion assay to test the capture and release of biologically active molecules from an azo electro-optic polymer, Poly (Disperse Red 1 methacrylate) (DR1/PMMA). The assay monitors the growth of bacteria placed in soft agar under a glass coverslip. Antibiotics can then be applied on the coverslip resulting in the clearance of the area under the coverslip due to growth inhibition. This assay demonstrates that DR1/PMMA is able to capture either tetracycline or ampicillin and the relative amount of DR1/PMMA required for capture was determined. Finally, the active antibiotics can be released from DR1/PMMA by exposure to green laser light. Exposure to white light from a torch or to heat does not release the antibiotic. |
| first_indexed | 2024-12-14T12:53:17Z |
| format | Article |
| id | doaj.art-c68498d4f3674834a8cce7996b63be11 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T12:53:17Z |
| publishDate | 2020-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c68498d4f3674834a8cce7996b63be112022-12-21T23:00:38ZengNature PortfolioScientific Reports2045-23222020-02-011011610.1038/s41598-020-60245-6Capture and light-induced release of antibiotics by an azo dye polymerStephen Atkins0Alysa Chueh1Taylor Barwell2Jean-Michel Nunzi3Laurent Seroude4Department of Biology, Queen’s UniversityDepartment of Chemistry, Queen’s UniversityDepartment of Biology, Queen’s UniversityDepartment of Chemistry, Queen’s UniversityDepartment of Biology, Queen’s UniversityAbstract The isomerisation of azo dyes can induce conformational changes which have potential applications in medicine and environmental protection. We developed an agar diffusion assay to test the capture and release of biologically active molecules from an azo electro-optic polymer, Poly (Disperse Red 1 methacrylate) (DR1/PMMA). The assay monitors the growth of bacteria placed in soft agar under a glass coverslip. Antibiotics can then be applied on the coverslip resulting in the clearance of the area under the coverslip due to growth inhibition. This assay demonstrates that DR1/PMMA is able to capture either tetracycline or ampicillin and the relative amount of DR1/PMMA required for capture was determined. Finally, the active antibiotics can be released from DR1/PMMA by exposure to green laser light. Exposure to white light from a torch or to heat does not release the antibiotic.https://doi.org/10.1038/s41598-020-60245-6 |
| spellingShingle | Stephen Atkins Alysa Chueh Taylor Barwell Jean-Michel Nunzi Laurent Seroude Capture and light-induced release of antibiotics by an azo dye polymer Scientific Reports |
| title | Capture and light-induced release of antibiotics by an azo dye polymer |
| title_full | Capture and light-induced release of antibiotics by an azo dye polymer |
| title_fullStr | Capture and light-induced release of antibiotics by an azo dye polymer |
| title_full_unstemmed | Capture and light-induced release of antibiotics by an azo dye polymer |
| title_short | Capture and light-induced release of antibiotics by an azo dye polymer |
| title_sort | capture and light induced release of antibiotics by an azo dye polymer |
| url | https://doi.org/10.1038/s41598-020-60245-6 |
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