Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico Studies
Research targeting natural cosmeceuticals is now increasing due to the safety and/or limited side effects of natural products that are highly valued in cosmetology. Within a research program exploring botanical sources for valuable skincare antioxidant components, the current study investigated the...
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MDPI AG
2023-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/19/6895 |
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| author | Hayam S. Ahmed Hala Abouzeid Mostafa A. Mansour Asmaa I. Owis Elham Amin Hany W. Darwish Ashwag S. Alanazi Ibrahim A. Naguib Naglaa Afifi |
| author_facet | Hayam S. Ahmed Hala Abouzeid Mostafa A. Mansour Asmaa I. Owis Elham Amin Hany W. Darwish Ashwag S. Alanazi Ibrahim A. Naguib Naglaa Afifi |
| author_sort | Hayam S. Ahmed |
| collection | DOAJ |
| description | Research targeting natural cosmeceuticals is now increasing due to the safety and/or limited side effects of natural products that are highly valued in cosmetology. Within a research program exploring botanical sources for valuable skincare antioxidant components, the current study investigated the phytochemical content and the biological potential of <i>Faucaria tuberculosa</i>. Phytochemical investigation of <i>F. tuberculosa</i> extract resulted in purification and characterization of six phytoconstituents, including a new one. The structure of the new constituent was elucidated as (-) catechin-(2→1′,4→2′)-phloroglucinol (<b>4</b>). The structural identity of all isolated compounds were confirmed on the basis of extensive physical and spectral (1D, 2D-NMR and HRESIMS) investigations. The ethanolic extract exhibits a rich content of total phenolics (TPC) and total flavonoids (TFC), estimated as 32 ± 0.034 mg GAE/g and 43 ± 0.004 mg RE/g, respectively. In addition, the antioxidant (ABTS and FRAP), antihyaluronidase and antityrosinase activities of all purified phytoconstituents were evaluated. The results noted (-) catechin-(2→1′,4→2′) phloroglucinol (<b>4</b>) and phloroglucinol (<b>1</b>) for their remarkable antioxidant activity, while isorhamnetin 3-<i>O</i>-rutinoside (<b>3</b>) and 3,5-dihydroxyphenyl <i>β</i>-D-glucopyranoside (<b>2</b>) achieved the most potent inhibitory activity against tyrosinase (IC<sub>50</sub> 22.09 ± 0.7 µM and 29.96 ± 0.44 µM, respectively) and hyaluronidase enzymes (IC<sub>50</sub> 49.30 ± 1.57 µM and 62.58 ± 0.92, respectively) that remarkably exceeds the activity of the standard drugs kojic acid (IC<sub>50</sub> = 65.21 ± 0.47 µM) and luteolin, (IC<sub>50</sub> = 116.16 ± 1.69 µM), respectively. A molecular docking study of the two active compounds (<b>3</b> and <b>2</b>) highlighted their high potential to bind to the active sites of the two enzymes involved in the study. |
| first_indexed | 2024-03-10T21:40:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:40:09Z |
| publishDate | 2023-09-01 |
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| spelling | doaj.art-c68d24873145499cafba7610702d10a52023-11-19T14:47:01ZengMDPI AGMolecules1420-30492023-09-012819689510.3390/molecules28196895Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico StudiesHayam S. Ahmed0Hala Abouzeid1Mostafa A. Mansour2Asmaa I. Owis3Elham Amin4Hany W. Darwish5Ashwag S. Alanazi6Ibrahim A. Naguib7Naglaa Afifi8Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Port Said University, Port Said 42515, EgyptDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Nahda University, Beni-Suef 62521, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptResearch targeting natural cosmeceuticals is now increasing due to the safety and/or limited side effects of natural products that are highly valued in cosmetology. Within a research program exploring botanical sources for valuable skincare antioxidant components, the current study investigated the phytochemical content and the biological potential of <i>Faucaria tuberculosa</i>. Phytochemical investigation of <i>F. tuberculosa</i> extract resulted in purification and characterization of six phytoconstituents, including a new one. The structure of the new constituent was elucidated as (-) catechin-(2→1′,4→2′)-phloroglucinol (<b>4</b>). The structural identity of all isolated compounds were confirmed on the basis of extensive physical and spectral (1D, 2D-NMR and HRESIMS) investigations. The ethanolic extract exhibits a rich content of total phenolics (TPC) and total flavonoids (TFC), estimated as 32 ± 0.034 mg GAE/g and 43 ± 0.004 mg RE/g, respectively. In addition, the antioxidant (ABTS and FRAP), antihyaluronidase and antityrosinase activities of all purified phytoconstituents were evaluated. The results noted (-) catechin-(2→1′,4→2′) phloroglucinol (<b>4</b>) and phloroglucinol (<b>1</b>) for their remarkable antioxidant activity, while isorhamnetin 3-<i>O</i>-rutinoside (<b>3</b>) and 3,5-dihydroxyphenyl <i>β</i>-D-glucopyranoside (<b>2</b>) achieved the most potent inhibitory activity against tyrosinase (IC<sub>50</sub> 22.09 ± 0.7 µM and 29.96 ± 0.44 µM, respectively) and hyaluronidase enzymes (IC<sub>50</sub> 49.30 ± 1.57 µM and 62.58 ± 0.92, respectively) that remarkably exceeds the activity of the standard drugs kojic acid (IC<sub>50</sub> = 65.21 ± 0.47 µM) and luteolin, (IC<sub>50</sub> = 116.16 ± 1.69 µM), respectively. A molecular docking study of the two active compounds (<b>3</b> and <b>2</b>) highlighted their high potential to bind to the active sites of the two enzymes involved in the study.https://www.mdpi.com/1420-3049/28/19/6895anti-aginganioxidant<i>Faucaria tuberculosa</i>hyaluronidase enzymetyrosinase enzymemolecular docking simulation |
| spellingShingle | Hayam S. Ahmed Hala Abouzeid Mostafa A. Mansour Asmaa I. Owis Elham Amin Hany W. Darwish Ashwag S. Alanazi Ibrahim A. Naguib Naglaa Afifi Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico Studies Molecules anti-aging anioxidant <i>Faucaria tuberculosa</i> hyaluronidase enzyme tyrosinase enzyme molecular docking simulation |
| title | Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico Studies |
| title_full | Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico Studies |
| title_fullStr | Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico Studies |
| title_full_unstemmed | Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico Studies |
| title_short | Antioxidant and Anti-Aging Phytoconstituents from <i>Faucaria tuberculosa</i>: In Vitro and In Silico Studies |
| title_sort | antioxidant and anti aging phytoconstituents from i faucaria tuberculosa i in vitro and in silico studies |
| topic | anti-aging anioxidant <i>Faucaria tuberculosa</i> hyaluronidase enzyme tyrosinase enzyme molecular docking simulation |
| url | https://www.mdpi.com/1420-3049/28/19/6895 |
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