Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy
Purpose. Oncolytic Reoviruses, as a self-limiting virus, can be used in cancer treatment, because they have the ability to replicate in tumor cells selectively and destroy them. Studies show that some immune response proteins may interfere with the virus life cycle. So, the main aim of this bioinfor...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi-Wiley
2022-01-01
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| Series: | Cellular Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/5756131 |
| _version_ | 1797958212389961728 |
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| author | Nasrin Rastegarvand Hoorieh Soleimanjahi Ehsan Arefian Mahmoud Reza Pourkarim |
| author_facet | Nasrin Rastegarvand Hoorieh Soleimanjahi Ehsan Arefian Mahmoud Reza Pourkarim |
| author_sort | Nasrin Rastegarvand |
| collection | DOAJ |
| description | Purpose. Oncolytic Reoviruses, as a self-limiting virus, can be used in cancer treatment, because they have the ability to replicate in tumor cells selectively and destroy them. Studies show that some immune response proteins may interfere with the virus life cycle. So, the main aim of this bioinformatic study is to check which microRNA is able to target some reovirus inhibitory proteins. Experimental Design. By use of online bioinformatics software, the microRNAs that could target inhibitory genes were selected. Then, other features like content ++ score and cell type were checked and finally the eligible microRNAs were determined. Results. After choosing 15 inhibitory proteins, analysis was performed and finally 37 microRNAs which could target inhibitory proteins in colorectal cell lines were selected. In the end, by investigation of web-based tools, just two microRNAs were finalized. Conclusions and Clinical Relevance. This bioinformatic study shows that microRNA-140 and microRNA-92a have the potential to target some inhibitory proteins which interfere with oncolytic Reovirus replication and it may help in the optimal use of this virus as a cancer treatment. Because selective reproduction of Reovirus in tumor cells, as a nonchemical therapy, can be a good way to overcome this disease with broad advantages. |
| first_indexed | 2024-04-11T00:16:51Z |
| format | Article |
| id | doaj.art-c68e670855b649d6888db794485f30ed |
| institution | Directory Open Access Journal |
| issn | 1462-5822 |
| language | English |
| last_indexed | 2024-04-11T00:16:51Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi-Wiley |
| record_format | Article |
| series | Cellular Microbiology |
| spelling | doaj.art-c68e670855b649d6888db794485f30ed2023-01-09T01:30:23ZengHindawi-WileyCellular Microbiology1462-58222022-01-01202210.1155/2022/5756131Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus EfficacyNasrin Rastegarvand0Hoorieh Soleimanjahi1Ehsan Arefian2Mahmoud Reza Pourkarim3Department of VirologyDepartment of VirologyDepartment of MicrobiologyLaboratory for Clinical and Epidemiological VirologyPurpose. Oncolytic Reoviruses, as a self-limiting virus, can be used in cancer treatment, because they have the ability to replicate in tumor cells selectively and destroy them. Studies show that some immune response proteins may interfere with the virus life cycle. So, the main aim of this bioinformatic study is to check which microRNA is able to target some reovirus inhibitory proteins. Experimental Design. By use of online bioinformatics software, the microRNAs that could target inhibitory genes were selected. Then, other features like content ++ score and cell type were checked and finally the eligible microRNAs were determined. Results. After choosing 15 inhibitory proteins, analysis was performed and finally 37 microRNAs which could target inhibitory proteins in colorectal cell lines were selected. In the end, by investigation of web-based tools, just two microRNAs were finalized. Conclusions and Clinical Relevance. This bioinformatic study shows that microRNA-140 and microRNA-92a have the potential to target some inhibitory proteins which interfere with oncolytic Reovirus replication and it may help in the optimal use of this virus as a cancer treatment. Because selective reproduction of Reovirus in tumor cells, as a nonchemical therapy, can be a good way to overcome this disease with broad advantages.http://dx.doi.org/10.1155/2022/5756131 |
| spellingShingle | Nasrin Rastegarvand Hoorieh Soleimanjahi Ehsan Arefian Mahmoud Reza Pourkarim Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy Cellular Microbiology |
| title | Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy |
| title_full | Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy |
| title_fullStr | Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy |
| title_full_unstemmed | Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy |
| title_short | Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy |
| title_sort | application of bioinformatics tools for the prediction of helper micrornas for improvement of oncolytic virus efficacy |
| url | http://dx.doi.org/10.1155/2022/5756131 |
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