Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model
Abstract Background Baylisascaris schroederi is the most common and harmful intestinal parasitic nematode of giant pandas, causing ascariasis. Although drug deworming is the main measure to control ascariasis in captive giant pandas, prolonged and repeated use of deworming drugs might induce resista...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-023-05886-y |
| _version_ | 1797769666803793920 |
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| author | Lang Xiong Ling Chen Yanxin Chen Nengxing Shen Ruiqi Hua Guangyou Yang |
| author_facet | Lang Xiong Ling Chen Yanxin Chen Nengxing Shen Ruiqi Hua Guangyou Yang |
| author_sort | Lang Xiong |
| collection | DOAJ |
| description | Abstract Background Baylisascaris schroederi is the most common and harmful intestinal parasitic nematode of giant pandas, causing ascariasis. Although drug deworming is the main measure to control ascariasis in captive giant pandas, prolonged and repeated use of deworming drugs might induce resistance in nematodes and drug residues in giant pandas. Therefore, developing a safe and effective vaccine might provide a novel strategy to prevent ascariasis in captive giant pandas. Methods Four highly expressed secretome genes encoding excretory and secretory proteins of B. schroederi, including transthyretin-like protein 46 (BsTLP), uncharacterized protein (BsUP), hypothetical protein 1 (BsHP1), and hypothetical protein 2 (BsHP2) and four functional genes [(encoding Galectin (BsGAL), glutathione S-transferase (BsGST), fatty acid-binding protein (BsFABP), and thioredoxin peroxidase (BsTPX)] were identified based on genome and transcriptome databases of B. schroederi and used to construct recombinant proteins via prokaryotic expression. Kunming mice were vaccinated subcutaneously twice with the recombinant proteins (50 μg/mouse) mixed with Quil A adjuvant with a 2-week interval and then orally challenged with 3000 infective eggs. The immunoprotective effects of the eight recombinant proteins on mice were assessed comprehensively using surface lesion histology scores of the mouse liver and lung, larval worm reduction, serum antibody levels (IgG, IgE, IgA, IgG1, and IgG2a), and cytokine production [interferon gamma (IFN-γ), interleukin (IL)-2, IL-4, IL-5, and IL-10]. Results Mice vaccinated with recombinant (r)BsUP (76.5%), rBsGAL (74.7%), and rBsHP2 (71.5%) showed a significant (P < 0.001) reduction in the larval worm rate compared with that in the adjuvant control. Besides, the surface lesions in the liver and lung of the vaccinated mice were alleviated. Serum levels of total IgG, IgE, IgA, IgG1, IgG2a, and cytokines, including IL-10, IL-5, and IFN-γ, were significantly higher (P < 0.001) than those in the control group. Conclusions The results showed that candidate three vaccines (rBsUP, rBsGAL, and rBsHP2) could provide effective protection against egg infection in mice associated with a mixed Th1/2-type immune response. Graphical Abstract |
| first_indexed | 2024-03-12T21:11:20Z |
| format | Article |
| id | doaj.art-c68ff4bd081543608d4aa6c439bd2036 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-12T21:11:20Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-c68ff4bd081543608d4aa6c439bd20362023-07-30T11:09:04ZengBMCParasites & Vectors1756-33052023-07-0116111310.1186/s13071-023-05886-yEvaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice modelLang Xiong0Ling Chen1Yanxin Chen2Nengxing Shen3Ruiqi Hua4Guangyou Yang5Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityAbstract Background Baylisascaris schroederi is the most common and harmful intestinal parasitic nematode of giant pandas, causing ascariasis. Although drug deworming is the main measure to control ascariasis in captive giant pandas, prolonged and repeated use of deworming drugs might induce resistance in nematodes and drug residues in giant pandas. Therefore, developing a safe and effective vaccine might provide a novel strategy to prevent ascariasis in captive giant pandas. Methods Four highly expressed secretome genes encoding excretory and secretory proteins of B. schroederi, including transthyretin-like protein 46 (BsTLP), uncharacterized protein (BsUP), hypothetical protein 1 (BsHP1), and hypothetical protein 2 (BsHP2) and four functional genes [(encoding Galectin (BsGAL), glutathione S-transferase (BsGST), fatty acid-binding protein (BsFABP), and thioredoxin peroxidase (BsTPX)] were identified based on genome and transcriptome databases of B. schroederi and used to construct recombinant proteins via prokaryotic expression. Kunming mice were vaccinated subcutaneously twice with the recombinant proteins (50 μg/mouse) mixed with Quil A adjuvant with a 2-week interval and then orally challenged with 3000 infective eggs. The immunoprotective effects of the eight recombinant proteins on mice were assessed comprehensively using surface lesion histology scores of the mouse liver and lung, larval worm reduction, serum antibody levels (IgG, IgE, IgA, IgG1, and IgG2a), and cytokine production [interferon gamma (IFN-γ), interleukin (IL)-2, IL-4, IL-5, and IL-10]. Results Mice vaccinated with recombinant (r)BsUP (76.5%), rBsGAL (74.7%), and rBsHP2 (71.5%) showed a significant (P < 0.001) reduction in the larval worm rate compared with that in the adjuvant control. Besides, the surface lesions in the liver and lung of the vaccinated mice were alleviated. Serum levels of total IgG, IgE, IgA, IgG1, IgG2a, and cytokines, including IL-10, IL-5, and IFN-γ, were significantly higher (P < 0.001) than those in the control group. Conclusions The results showed that candidate three vaccines (rBsUP, rBsGAL, and rBsHP2) could provide effective protection against egg infection in mice associated with a mixed Th1/2-type immune response. Graphical Abstracthttps://doi.org/10.1186/s13071-023-05886-yRecombinant proteinsBaylisascaris schroederiGiant pandaImmunoprotective effectMouse model |
| spellingShingle | Lang Xiong Ling Chen Yanxin Chen Nengxing Shen Ruiqi Hua Guangyou Yang Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model Parasites & Vectors Recombinant proteins Baylisascaris schroederi Giant panda Immunoprotective effect Mouse model |
| title | Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model |
| title_full | Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model |
| title_fullStr | Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model |
| title_full_unstemmed | Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model |
| title_short | Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model |
| title_sort | evaluation of the immunoprotective effects of eight recombinant proteins from baylisascaris schroederi in mice model |
| topic | Recombinant proteins Baylisascaris schroederi Giant panda Immunoprotective effect Mouse model |
| url | https://doi.org/10.1186/s13071-023-05886-y |
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