Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals
BackgroundVIM metallo-β-lactamases are enzymes characterized by the ability to hydrolyze all β-lactams. Usually, blaVIM-like genes are carried by class 1 integrons. In the Czech Republic, only sporadic cases of VIM-producing Enterobacterales have been reported in which those isolates carried the VIM...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.993240/full |
| _version_ | 1797962163477807104 |
|---|---|
| author | Ibrahim Bitar Ibrahim Bitar Costas C. Papagiannitsis Lucie Kraftova Lucie Kraftova Vittoria Mattioni Marchetti Vittoria Mattioni Marchetti Efthymia Petinaki Marc Finianos Marc Finianos Katerina Chudejova Katerina Chudejova Helena Zemlickova Helena Zemlickova Jaroslav Hrabak Jaroslav Hrabak |
| author_facet | Ibrahim Bitar Ibrahim Bitar Costas C. Papagiannitsis Lucie Kraftova Lucie Kraftova Vittoria Mattioni Marchetti Vittoria Mattioni Marchetti Efthymia Petinaki Marc Finianos Marc Finianos Katerina Chudejova Katerina Chudejova Helena Zemlickova Helena Zemlickova Jaroslav Hrabak Jaroslav Hrabak |
| author_sort | Ibrahim Bitar |
| collection | DOAJ |
| description | BackgroundVIM metallo-β-lactamases are enzymes characterized by the ability to hydrolyze all β-lactams. Usually, blaVIM-like genes are carried by class 1 integrons. In the Czech Republic, only sporadic cases of VIM-producing Enterobacterales have been reported in which those isolates carried the VIM-1 carbapenemase-encoding integron In110. However, during 2019–2020, an increased number was reported. Therefore, the aim of the current study was to characterize the genetic elements involved in the increased spread of blaVIM genes.Materials and methods32 VIM-producing Enterobacterales collected between 2019 and 2020 were subjected to: antimicrobial susceptibility testing, integron analysis, and short reads sequencing. Based on the results, 19 isolates were selected as representative and sequenced using Sequel I platform.ResultsThe 32 VIM-producing isolates exhibited variations in the MICs of carbapenems. Based on short-read data, 26 of the 32 sequenced isolates harbored the blaVIM-1 allele while six isolates carried the blaVIM-4 gene. The most prevalent was the In110 integron (n = 24) and two isolates carried the In4873 class 1 integron. The blaVIM-4 allele was identified in class 1 integrons In1174 (n = 3), In416 (n = 1), In2143 (n = 1) and In2150. Long reads sequencing revealed that the blaVIM was carried by: pKPC-CAV1193-like (n = 6), HI1 (pNDM-CIT; n = 4), HI2 (n = 3), FIB (pECLA; n = 2) and N (n = 1) incompatibility groups. Two blaVIM-carrying plasmids could not be typed by the database, while another one was integrated into the chromosome.ConclusionWe observed the spread of VIM-encoding integrons, mainly of In110, among Enterobacterales isolated from Czech hospitals, but also an increased number of novel elements underlining the ongoing evolution. |
| first_indexed | 2024-04-11T01:09:02Z |
| format | Article |
| id | doaj.art-c690771e3405470c974f47f29327d4eb |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T01:09:02Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c690771e3405470c974f47f29327d4eb2023-01-04T10:26:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.993240993240Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitalsIbrahim Bitar0Ibrahim Bitar1Costas C. Papagiannitsis2Lucie Kraftova3Lucie Kraftova4Vittoria Mattioni Marchetti5Vittoria Mattioni Marchetti6Efthymia Petinaki7Marc Finianos8Marc Finianos9Katerina Chudejova10Katerina Chudejova11Helena Zemlickova12Helena Zemlickova13Jaroslav Hrabak14Jaroslav Hrabak15Department of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, University Hospital of Larissa, Larissa, GreeceDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, University Hospital of Larissa, Larissa, GreeceDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaNational Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, CzechiaDepartment of Medical Microbiology, 3rd Faculty of Medicine, Charles University, Prague, CzechiaDepartment of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, CzechiaBiomedical Center, Faculty of Medicine, Charles University, Pilsen, CzechiaBackgroundVIM metallo-β-lactamases are enzymes characterized by the ability to hydrolyze all β-lactams. Usually, blaVIM-like genes are carried by class 1 integrons. In the Czech Republic, only sporadic cases of VIM-producing Enterobacterales have been reported in which those isolates carried the VIM-1 carbapenemase-encoding integron In110. However, during 2019–2020, an increased number was reported. Therefore, the aim of the current study was to characterize the genetic elements involved in the increased spread of blaVIM genes.Materials and methods32 VIM-producing Enterobacterales collected between 2019 and 2020 were subjected to: antimicrobial susceptibility testing, integron analysis, and short reads sequencing. Based on the results, 19 isolates were selected as representative and sequenced using Sequel I platform.ResultsThe 32 VIM-producing isolates exhibited variations in the MICs of carbapenems. Based on short-read data, 26 of the 32 sequenced isolates harbored the blaVIM-1 allele while six isolates carried the blaVIM-4 gene. The most prevalent was the In110 integron (n = 24) and two isolates carried the In4873 class 1 integron. The blaVIM-4 allele was identified in class 1 integrons In1174 (n = 3), In416 (n = 1), In2143 (n = 1) and In2150. Long reads sequencing revealed that the blaVIM was carried by: pKPC-CAV1193-like (n = 6), HI1 (pNDM-CIT; n = 4), HI2 (n = 3), FIB (pECLA; n = 2) and N (n = 1) incompatibility groups. Two blaVIM-carrying plasmids could not be typed by the database, while another one was integrated into the chromosome.ConclusionWe observed the spread of VIM-encoding integrons, mainly of In110, among Enterobacterales isolated from Czech hospitals, but also an increased number of novel elements underlining the ongoing evolution.https://www.frontiersin.org/articles/10.3389/fmicb.2022.993240/fullEnterobacteraleswhole-genome sequencingblaVIM-1blaVIM-4In110plasmids |
| spellingShingle | Ibrahim Bitar Ibrahim Bitar Costas C. Papagiannitsis Lucie Kraftova Lucie Kraftova Vittoria Mattioni Marchetti Vittoria Mattioni Marchetti Efthymia Petinaki Marc Finianos Marc Finianos Katerina Chudejova Katerina Chudejova Helena Zemlickova Helena Zemlickova Jaroslav Hrabak Jaroslav Hrabak Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals Frontiers in Microbiology Enterobacterales whole-genome sequencing blaVIM-1 blaVIM-4 In110 plasmids |
| title | Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals |
| title_full | Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals |
| title_fullStr | Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals |
| title_full_unstemmed | Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals |
| title_short | Implication of different replicons in the spread of the VIM-1-encoding integron, In110, in Enterobacterales from Czech hospitals |
| title_sort | implication of different replicons in the spread of the vim 1 encoding integron in110 in enterobacterales from czech hospitals |
| topic | Enterobacterales whole-genome sequencing blaVIM-1 blaVIM-4 In110 plasmids |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.993240/full |
| work_keys_str_mv | AT ibrahimbitar implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT ibrahimbitar implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT costascpapagiannitsis implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT luciekraftova implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT luciekraftova implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT vittoriamattionimarchetti implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT vittoriamattionimarchetti implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT efthymiapetinaki implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT marcfinianos implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT marcfinianos implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT katerinachudejova implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT katerinachudejova implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT helenazemlickova implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT helenazemlickova implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT jaroslavhrabak implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals AT jaroslavhrabak implicationofdifferentrepliconsinthespreadofthevim1encodingintegronin110inenterobacteralesfromczechhospitals |