Expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma
Background/Aim. Deregulation of the normal cell cycle is common in upper urothelial carcinoma (UUC). The aim of this study was to investigate the expression of regulatory proteins of the cell cycle (p53, p16, cyclin D1, HER-2) and proliferative Ki-67 activity in UUC, and to determine their inter...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2011-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2011/0042-84501107567D.pdf |
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| author | Dolićanin Zana Janković-Veličković Ljubinka Đorđević Biljana Višnjić Milan Pešić Ivana Ristić Ana Marjanović Vesna |
| author_facet | Dolićanin Zana Janković-Veličković Ljubinka Đorđević Biljana Višnjić Milan Pešić Ivana Ristić Ana Marjanović Vesna |
| author_sort | Dolićanin Zana |
| collection | DOAJ |
| description | Background/Aim. Deregulation of the normal cell cycle is common in upper urothelial carcinoma (UUC). The aim of this study was to investigate the expression of regulatory proteins of the cell cycle (p53, p16, cyclin D1, HER-2) and proliferative Ki-67 activity in UUC, and to determine their interaction and influence on the phenotypic characteristics of UUC. Methods. In 44 patients with UUC, histopathological and immunohistochemical analyses (p53, p16, cyclin D1, HER-2, and Ki-67) of tumors were done. Results. Overexpression/ altered expression of p53, p16, cyclin D1 or HER-2 was detected in 20%, 57%, 64%, and 57% of tumors, respectively. Eleven (25%) UUC had a high proliferative Ki-67 index. Forty patients (91%) had at least one marker altered, while four (9%) tumors had a wild-type status. Analysis of relationship between expressions of molecular markers showed that only high expression of p53 was significantly associated with altered p16 activity (p < 0.05). High Ki-67 index was associated with the high stage (p < 0.005), solid growth (p < 0.01), high grade (p < 0.05), and multifocality p < 0.05) of UUC, while high expression of p53 was associated with the solid growth (p < 0.05). In regression models that included all molecular markers and phenotypic characteristics, only Ki-67 correlated with the growth (p < 0.0001), stage (p < 0.01), grade (p < 0.05) and multifocality (p < 0.05) of UUC; Ki-67 and HER-2 expression correlated with the lymphovascular invasion (p < 0.05). Conclusions. This investigation showed that only negative regulatory proteins of the cell cycle, p53 and p16, were significantly associated in UUC, while proliferative marker Ki-67 was in relation to the key phenotypic characteristics of UUC in the best way. |
| first_indexed | 2024-12-10T03:49:28Z |
| format | Article |
| id | doaj.art-c691ed2506c44cbcb91b5ef7c5e460d9 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 |
| language | English |
| last_indexed | 2024-12-10T03:49:28Z |
| publishDate | 2011-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-c691ed2506c44cbcb91b5ef7c5e460d92022-12-22T02:03:18ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502011-01-0168756757410.2298/VSP1107567DExpression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinomaDolićanin ZanaJanković-Veličković LjubinkaĐorđević BiljanaVišnjić MilanPešić IvanaRistić AnaMarjanović VesnaBackground/Aim. Deregulation of the normal cell cycle is common in upper urothelial carcinoma (UUC). The aim of this study was to investigate the expression of regulatory proteins of the cell cycle (p53, p16, cyclin D1, HER-2) and proliferative Ki-67 activity in UUC, and to determine their interaction and influence on the phenotypic characteristics of UUC. Methods. In 44 patients with UUC, histopathological and immunohistochemical analyses (p53, p16, cyclin D1, HER-2, and Ki-67) of tumors were done. Results. Overexpression/ altered expression of p53, p16, cyclin D1 or HER-2 was detected in 20%, 57%, 64%, and 57% of tumors, respectively. Eleven (25%) UUC had a high proliferative Ki-67 index. Forty patients (91%) had at least one marker altered, while four (9%) tumors had a wild-type status. Analysis of relationship between expressions of molecular markers showed that only high expression of p53 was significantly associated with altered p16 activity (p < 0.05). High Ki-67 index was associated with the high stage (p < 0.005), solid growth (p < 0.01), high grade (p < 0.05), and multifocality p < 0.05) of UUC, while high expression of p53 was associated with the solid growth (p < 0.05). In regression models that included all molecular markers and phenotypic characteristics, only Ki-67 correlated with the growth (p < 0.0001), stage (p < 0.01), grade (p < 0.05) and multifocality (p < 0.05) of UUC; Ki-67 and HER-2 expression correlated with the lymphovascular invasion (p < 0.05). Conclusions. This investigation showed that only negative regulatory proteins of the cell cycle, p53 and p16, were significantly associated in UUC, while proliferative marker Ki-67 was in relation to the key phenotypic characteristics of UUC in the best way.http://www.doiserbia.nb.rs/img/doi/0042-8450/2011/0042-84501107567D.pdfurinary bladdercarcinomacell cycle proteinsKi-67antigen |
| spellingShingle | Dolićanin Zana Janković-Veličković Ljubinka Đorđević Biljana Višnjić Milan Pešić Ivana Ristić Ana Marjanović Vesna Expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma Vojnosanitetski Pregled urinary bladder carcinoma cell cycle proteins Ki-67antigen |
| title | Expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma |
| title_full | Expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma |
| title_fullStr | Expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma |
| title_full_unstemmed | Expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma |
| title_short | Expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma |
| title_sort | expression of regulatory proteins and proliferative activity in relation to phenotypic characteristics of upper urothelial carcinoma |
| topic | urinary bladder carcinoma cell cycle proteins Ki-67antigen |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2011/0042-84501107567D.pdf |
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