Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition
In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/9/347 |
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| author | Carrie L. Jenkins Heather D. Bean |
| author_facet | Carrie L. Jenkins Heather D. Bean |
| author_sort | Carrie L. Jenkins |
| collection | DOAJ |
| description | In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>. <i>S. aureus</i> (ATCC 12600) and <i>S. epidermidis</i> (ATCC 12228) were cultured in triplicate in four rich complex media (brain heart infusion (BHI), lysogeny broth (LB), Mueller Hinton broth (MHB), and tryptic soy broth (TSB)), and the volatile metabolites produced by each culture were analyzed using headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography—time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS). When comparing the chemical compositions of the staph volatilomes by the presence versus absence of volatiles produced in each medium, we observed few differences. However, when the relative abundances of volatiles were included in the analyses, we observed that culturing staph in media containing free glucose (BHI and TSB) resulted in volatilomes dominated by acids and esters (67%). The low-glucose media (LB and MHB) produced ketones in greatest relative abundances, but the volatilome compositions in these two media were highly dissimilar. We conclude that the staphylococcal volatilome is strongly influenced by the nutritional composition of the growth medium, especially the availability of free glucose, which is much more evident when the relative abundances of the volatiles are analyzed, compared to the presence versus absence. |
| first_indexed | 2024-03-10T16:47:11Z |
| format | Article |
| id | doaj.art-c693981533ab490f9ba61f7031178e4d |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T16:47:11Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-c693981533ab490f9ba61f7031178e4d2023-11-20T11:32:26ZengMDPI AGMetabolites2218-19892020-08-0110934710.3390/metabo10090347Dependence of the Staphylococcal Volatilome Composition on Microbial NutritionCarrie L. Jenkins0Heather D. Bean1School of Life Sciences, Arizona State University, Tempe, AZ 85287, USASchool of Life Sciences, Arizona State University, Tempe, AZ 85287, USAIn vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>. <i>S. aureus</i> (ATCC 12600) and <i>S. epidermidis</i> (ATCC 12228) were cultured in triplicate in four rich complex media (brain heart infusion (BHI), lysogeny broth (LB), Mueller Hinton broth (MHB), and tryptic soy broth (TSB)), and the volatile metabolites produced by each culture were analyzed using headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography—time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS). When comparing the chemical compositions of the staph volatilomes by the presence versus absence of volatiles produced in each medium, we observed few differences. However, when the relative abundances of volatiles were included in the analyses, we observed that culturing staph in media containing free glucose (BHI and TSB) resulted in volatilomes dominated by acids and esters (67%). The low-glucose media (LB and MHB) produced ketones in greatest relative abundances, but the volatilome compositions in these two media were highly dissimilar. We conclude that the staphylococcal volatilome is strongly influenced by the nutritional composition of the growth medium, especially the availability of free glucose, which is much more evident when the relative abundances of the volatiles are analyzed, compared to the presence versus absence.https://www.mdpi.com/2218-1989/10/9/347<i>Staphylococcus aureus</i><i>Staphylococcus epidermidis</i>volatile organic compoundsGC×GC-TOFMScatabolite repression control |
| spellingShingle | Carrie L. Jenkins Heather D. Bean Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition Metabolites <i>Staphylococcus aureus</i> <i>Staphylococcus epidermidis</i> volatile organic compounds GC×GC-TOFMS catabolite repression control |
| title | Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition |
| title_full | Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition |
| title_fullStr | Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition |
| title_full_unstemmed | Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition |
| title_short | Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition |
| title_sort | dependence of the staphylococcal volatilome composition on microbial nutrition |
| topic | <i>Staphylococcus aureus</i> <i>Staphylococcus epidermidis</i> volatile organic compounds GC×GC-TOFMS catabolite repression control |
| url | https://www.mdpi.com/2218-1989/10/9/347 |
| work_keys_str_mv | AT carrieljenkins dependenceofthestaphylococcalvolatilomecompositiononmicrobialnutrition AT heatherdbean dependenceofthestaphylococcalvolatilomecompositiononmicrobialnutrition |