Partial structural characterization of pectin cell wall from Argania spinosa leaves
The pectin polysaccharides from leaves of Argania spinosa (L.) Skeels, collected from Stidia area in the west coast of northern Algeria, were investigated by using sequential extractions and the resulting fractions were analysed for monosaccharide composition and chemical structure. Water-soluble pe...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844015303443 |
| _version_ | 1828384502837149696 |
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| author | Kadda Hachem Yasmina Benabdesslem Samia Ghomari Okkacha Hasnaoui Meriem Kaid-Harche |
| author_facet | Kadda Hachem Yasmina Benabdesslem Samia Ghomari Okkacha Hasnaoui Meriem Kaid-Harche |
| author_sort | Kadda Hachem |
| collection | DOAJ |
| description | The pectin polysaccharides from leaves of Argania spinosa (L.) Skeels, collected from Stidia area in the west coast of northern Algeria, were investigated by using sequential extractions and the resulting fractions were analysed for monosaccharide composition and chemical structure. Water-soluble pectic (ALS-WSP) and chelating-soluble pectic (ALS-CSP) fractions were obtained, de-esterified and fractionated by anion-exchange chromatography and characterized by sugar analysis combined with methylation analysis and 1H and 13C NMR spectroscopy. The data reveal the presence of altering homogalacturonan (HG) and rhamnogalacturonan I (RG-I) in both pectin fraction. The rhamnogalacturonan I (RG-I) are consisted of a disaccharide repeating unit [→ α-D-GalpA-1,2-α-L-Rhap-1,4 →] backbone, with side chains contained highly branched α-(1 → 5)-linked arabinan and short linear β-(1 → 4)-linked galactan, attached to O-4 of the rhamnosyl residues. |
| first_indexed | 2024-12-10T05:04:24Z |
| format | Article |
| id | doaj.art-c69666a6209b46e9bfc9b47e2b0bc55c |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T05:04:24Z |
| publishDate | 2016-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c69666a6209b46e9bfc9b47e2b0bc55c2022-12-22T02:01:18ZengElsevierHeliyon2405-84402016-02-012210.1016/j.heliyon.2016.e00076Partial structural characterization of pectin cell wall from Argania spinosa leavesKadda Hachem0Yasmina Benabdesslem1Samia Ghomari2Okkacha Hasnaoui3Meriem Kaid-Harche4Laboratoire des Productions, Valorisations Végétales et Microbiennes, Département de Biotechnologie, Université des Sciences et de la Technologie d’Oran, BP 1505, El Mnaouar, 31000 Oran, AlgeriaDépartement de Biologie, Faculté des Sciences, Université Docteur Moulay Tahar de Saida, BP 138 cité ENNASR, 20000 Saida, AlgeriaFaculté des Sciences, Université de Djillali Liabès Sidi-Bel-Abbès, BP 89, 22000 Sidi-Bel-Abbés, AlgériaDépartement de Biologie, Faculté des Sciences, Université Docteur Moulay Tahar de Saida, BP 138 cité ENNASR, 20000 Saida, AlgeriaLaboratoire des Productions, Valorisations Végétales et Microbiennes, Département de Biotechnologie, Université des Sciences et de la Technologie d’Oran, BP 1505, El Mnaouar, 31000 Oran, AlgeriaThe pectin polysaccharides from leaves of Argania spinosa (L.) Skeels, collected from Stidia area in the west coast of northern Algeria, were investigated by using sequential extractions and the resulting fractions were analysed for monosaccharide composition and chemical structure. Water-soluble pectic (ALS-WSP) and chelating-soluble pectic (ALS-CSP) fractions were obtained, de-esterified and fractionated by anion-exchange chromatography and characterized by sugar analysis combined with methylation analysis and 1H and 13C NMR spectroscopy. The data reveal the presence of altering homogalacturonan (HG) and rhamnogalacturonan I (RG-I) in both pectin fraction. The rhamnogalacturonan I (RG-I) are consisted of a disaccharide repeating unit [→ α-D-GalpA-1,2-α-L-Rhap-1,4 →] backbone, with side chains contained highly branched α-(1 → 5)-linked arabinan and short linear β-(1 → 4)-linked galactan, attached to O-4 of the rhamnosyl residues.http://www.sciencedirect.com/science/article/pii/S2405844015303443BiochemistryApplied sciencesPlant biologyNatural product chemistryBiological sciences |
| spellingShingle | Kadda Hachem Yasmina Benabdesslem Samia Ghomari Okkacha Hasnaoui Meriem Kaid-Harche Partial structural characterization of pectin cell wall from Argania spinosa leaves Heliyon Biochemistry Applied sciences Plant biology Natural product chemistry Biological sciences |
| title | Partial structural characterization of pectin cell wall from Argania spinosa leaves |
| title_full | Partial structural characterization of pectin cell wall from Argania spinosa leaves |
| title_fullStr | Partial structural characterization of pectin cell wall from Argania spinosa leaves |
| title_full_unstemmed | Partial structural characterization of pectin cell wall from Argania spinosa leaves |
| title_short | Partial structural characterization of pectin cell wall from Argania spinosa leaves |
| title_sort | partial structural characterization of pectin cell wall from argania spinosa leaves |
| topic | Biochemistry Applied sciences Plant biology Natural product chemistry Biological sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2405844015303443 |
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