Association of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan women
Breast cancer is the most prevalent neoplasm and the second leading cause of death among females in Kenya. Estrogen and its metabolites are known risk factors for breast cancer. Polymorphisms in these genes and breast cancer susceptibility are unique among different populations. This study aimed to...
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| Format: | Article |
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University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300005M.pdf |
| _version_ | 1797807609521111040 |
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| author | Murithi Mary Nyanjom Steven Mobegi Victor Shahin Sayed Makokha Francis |
| author_facet | Murithi Mary Nyanjom Steven Mobegi Victor Shahin Sayed Makokha Francis |
| author_sort | Murithi Mary |
| collection | DOAJ |
| description | Breast cancer is the most prevalent neoplasm and the second leading cause of death among females in Kenya. Estrogen and its metabolites are known risk factors for breast cancer. Polymorphisms in these genes and breast cancer susceptibility are unique among different populations. This study aimed to determine the probable associations between estrogen-metabolizing gene variations and other risk factors for breast cancer risk in Kenyan women. Buffy coat samples were obtained from patients diagnosed with estrogen receptor-positive breast cancer, benign breast disease, and healthy volunteers. Genotyping of target polymorphisms was conducted using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis. The rs4646903 variant genotype CC was associated with breast cancer in the case-control model (P=0.001); the heterozygous genotype TC (P=0.01) and the luminal B molecular subtype (P=0.02) showed increased odds of late-stage breast cancer. The rs1048943 variant genotype GG was associated with breast cancer in the case-benign model (P=0.04), whereas CG was associated with breast cancer in the case-control model (P=0.02). These findings imply that the rs4646903 and rs1048943 variant genotypes are involved in breast cancer risk in Kenyan women. Hence, they may be explored further as potential markers for the disease. |
| first_indexed | 2024-03-13T06:25:09Z |
| format | Article |
| id | doaj.art-c696746ff53f4256947b5e90f2c9123c |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-03-13T06:25:09Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-c696746ff53f4256947b5e90f2c9123c2023-06-09T10:41:58ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-01751576710.2298/ABS230115005M0354-46642300005MAssociation of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan womenMurithi Mary0Nyanjom Steven1Mobegi Victor2Shahin Sayed3Makokha Francis4Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya + Pre-Clinical Department, Kabarak University, Nakuru, KenyaBiochemistry Department, Jomo Kenyatta University of Agriculture and Technology, Nairobi, KenyaDepartment of Biochemistry, University of Nairobi, Nairobi, KenyaDepartment of Pathology, Aga Khan University Hospital, Nairobi, KenyaDirectorate of Research and Innovation, Mount Kenya University, Thika, KenyaBreast cancer is the most prevalent neoplasm and the second leading cause of death among females in Kenya. Estrogen and its metabolites are known risk factors for breast cancer. Polymorphisms in these genes and breast cancer susceptibility are unique among different populations. This study aimed to determine the probable associations between estrogen-metabolizing gene variations and other risk factors for breast cancer risk in Kenyan women. Buffy coat samples were obtained from patients diagnosed with estrogen receptor-positive breast cancer, benign breast disease, and healthy volunteers. Genotyping of target polymorphisms was conducted using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis. The rs4646903 variant genotype CC was associated with breast cancer in the case-control model (P=0.001); the heterozygous genotype TC (P=0.01) and the luminal B molecular subtype (P=0.02) showed increased odds of late-stage breast cancer. The rs1048943 variant genotype GG was associated with breast cancer in the case-benign model (P=0.04), whereas CG was associated with breast cancer in the case-control model (P=0.02). These findings imply that the rs4646903 and rs1048943 variant genotypes are involved in breast cancer risk in Kenyan women. Hence, they may be explored further as potential markers for the disease.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300005M.pdfbreast cancergene polymorphismsestrogen metabolizing genepolymerase chain reaction-restriction fragment length polymorphismgenotype |
| spellingShingle | Murithi Mary Nyanjom Steven Mobegi Victor Shahin Sayed Makokha Francis Association of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan women Archives of Biological Sciences breast cancer gene polymorphisms estrogen metabolizing gene polymerase chain reaction-restriction fragment length polymorphism genotype |
| title | Association of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan women |
| title_full | Association of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan women |
| title_fullStr | Association of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan women |
| title_full_unstemmed | Association of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan women |
| title_short | Association of rs4646903 and rs1048943 CYP1A1 estrogen-metabolizing gene polymorphisms with estrogen receptor-positive breast cancer in Kenyan women |
| title_sort | association of rs4646903 and rs1048943 cyp1a1 estrogen metabolizing gene polymorphisms with estrogen receptor positive breast cancer in kenyan women |
| topic | breast cancer gene polymorphisms estrogen metabolizing gene polymerase chain reaction-restriction fragment length polymorphism genotype |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300005M.pdf |
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