Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino Rats
Background: Diabetes Mellitus (DM) is a worldwide common disease and it is considered a serious public health disorder .The reproductive function is highly sensitive to changes in metabolic status, with defective reproductive capacity. Aim of Work: To demonstrate the histological changes in the pros...
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| Format: | Article |
| Language: | English |
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Beni-Suef University, Faculty of Medicine
2021-06-01
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| Series: | Egyptian Journal of Medical Research |
| Subjects: | |
| Online Access: | https://ejmr.journals.ekb.eg/article_175725_f4c39eb1afb615ee65d30a70b779c044.pdf |
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| author | Mohammed Ahmed Abd-El Hafez Safinaz Salah El-Din Sayed Dalia Hussein Abd El Azize Amira Shaban Gaeidy Rashwan |
| author_facet | Mohammed Ahmed Abd-El Hafez Safinaz Salah El-Din Sayed Dalia Hussein Abd El Azize Amira Shaban Gaeidy Rashwan |
| author_sort | Mohammed Ahmed Abd-El Hafez |
| collection | DOAJ |
| description | Background: Diabetes Mellitus (DM) is a worldwide common disease and it is considered a serious public health disorder .The reproductive function is highly sensitive to changes in metabolic status, with defective reproductive capacity. Aim of Work: To demonstrate the histological changes in the prostate of rats with STZ induced diabetes type and to examine to what extent insulin could reverse such changes. Materials and methods: Twenty six male albino rats were divided into three groups. Group I: control; Group II: Diabetic was exposed to induction of diabetes by a single intraperitoneal injection of Streptozotocin(STZ) 60 mg/kg in 0.1 M citrate buffer; Group III: Diabetic then received insulin (3IU/day S.C( (for 4 weeks). The rats of each group were sacrificed after 4 weeks. The prostates were processed for electron microscopic sections and other sections stained with H&E, Masson's trichrome, immunohistochemical staining of α smooth muscle actin, Vimentin and PCNA and statistical analysis were applied. Results: Diabetes resulted in deterioration of normal structure of the prostatic acini and some prostatic acini showed cell proliferation with increase Masson's trichrome staining and increase immunoreactivity for α smooth muscle actin, Vimentin and PCNA. Electron microscopic examination reavealed dilatation of the cisternae of the Golgi complex, endoplasmic reticulum, and some epithelial cells showed proliferation and irregular contour of their nuclei. Insulin administration mostly reversed these changes. Conclusion: insulin administration greatly improved the histological architecture of the prostate gland. |
| first_indexed | 2024-04-10T07:02:05Z |
| format | Article |
| id | doaj.art-c69c2a4f9bf64843938625c69fe85b3b |
| institution | Directory Open Access Journal |
| issn | 2682-4396 2682-440X |
| language | English |
| last_indexed | 2024-04-10T07:02:05Z |
| publishDate | 2021-06-01 |
| publisher | Beni-Suef University, Faculty of Medicine |
| record_format | Article |
| series | Egyptian Journal of Medical Research |
| spelling | doaj.art-c69c2a4f9bf64843938625c69fe85b3b2023-02-27T17:41:46ZengBeni-Suef University, Faculty of MedicineEgyptian Journal of Medical Research2682-43962682-440X2021-06-012220322610.21608/ejmr.2021.175725175725Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino RatsMohammed Ahmed Abd-El Hafez0Safinaz Salah El-Din Sayed1Dalia Hussein Abd El Azize2Amira Shaban Gaeidy Rashwan3Histology department, Faculty of Medicine, Cairo University, Egypt.Histology department, Faculty of Medicine, Cairo University, Egypt.Histology department, Faculty of Medicine, Beni-Suef University, Egypt.Histology department, Faculty of Medicine, Beni-Suef University, Egypt.Background: Diabetes Mellitus (DM) is a worldwide common disease and it is considered a serious public health disorder .The reproductive function is highly sensitive to changes in metabolic status, with defective reproductive capacity. Aim of Work: To demonstrate the histological changes in the prostate of rats with STZ induced diabetes type and to examine to what extent insulin could reverse such changes. Materials and methods: Twenty six male albino rats were divided into three groups. Group I: control; Group II: Diabetic was exposed to induction of diabetes by a single intraperitoneal injection of Streptozotocin(STZ) 60 mg/kg in 0.1 M citrate buffer; Group III: Diabetic then received insulin (3IU/day S.C( (for 4 weeks). The rats of each group were sacrificed after 4 weeks. The prostates were processed for electron microscopic sections and other sections stained with H&E, Masson's trichrome, immunohistochemical staining of α smooth muscle actin, Vimentin and PCNA and statistical analysis were applied. Results: Diabetes resulted in deterioration of normal structure of the prostatic acini and some prostatic acini showed cell proliferation with increase Masson's trichrome staining and increase immunoreactivity for α smooth muscle actin, Vimentin and PCNA. Electron microscopic examination reavealed dilatation of the cisternae of the Golgi complex, endoplasmic reticulum, and some epithelial cells showed proliferation and irregular contour of their nuclei. Insulin administration mostly reversed these changes. Conclusion: insulin administration greatly improved the histological architecture of the prostate gland.https://ejmr.journals.ekb.eg/article_175725_f4c39eb1afb615ee65d30a70b779c044.pdfdiabetesinsulinprostateimmunohistochemistryelectron microscope |
| spellingShingle | Mohammed Ahmed Abd-El Hafez Safinaz Salah El-Din Sayed Dalia Hussein Abd El Azize Amira Shaban Gaeidy Rashwan Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino Rats Egyptian Journal of Medical Research diabetes insulin prostate immunohistochemistry electron microscope |
| title | Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino Rats |
| title_full | Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino Rats |
| title_fullStr | Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino Rats |
| title_full_unstemmed | Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino Rats |
| title_short | Histological Study on The effect of Diabetes and Insulin Replacement Therapy on The prostate of Albino Rats |
| title_sort | histological study on the effect of diabetes and insulin replacement therapy on the prostate of albino rats |
| topic | diabetes insulin prostate immunohistochemistry electron microscope |
| url | https://ejmr.journals.ekb.eg/article_175725_f4c39eb1afb615ee65d30a70b779c044.pdf |
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