Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey
Background: Dirofilaria immitis and Dirofilaria repens are the most common species of filarial nematodes described in the dogs. A single-step multiplex PCR was applied to detect and differentiate simultaneously and unequivocally D. immitis and D. repens on DNA extracted from canine peripheral blood...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-10-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/324 |
| _version_ | 1828554361891979264 |
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| author | Sami Simsek Ayse Turkan Ciftci |
| author_facet | Sami Simsek Ayse Turkan Ciftci |
| author_sort | Sami Simsek |
| collection | DOAJ |
| description | Background: Dirofilaria immitis and Dirofilaria repens are the most common species of filarial nematodes described in the dogs. A single-step multiplex PCR was applied to detect and differentiate simultaneously and unequivocally D. immitis and D. repens on DNA extracted from canine peripheral blood and besides to detect the seroprevalance of D. immitis by ELISA in Elazig Province, Turkey. A PCR detection of the Wolbachia, which plays an important role in D. immitis biology and contributes to the inflammatory pathology of the heartworm, was also applied for the first time in Turkey.
Methods: A total of 161 whole blood and sera samples were collected from stray dogs and stored at -20 °C until used. After DNA extraction, all samples were processed with Dirofilaria primers by multiplex-PCR and Wolbachia primers by conventional PCR besides ELISA for serology. The amplification was performed using a set of primers designed on a portion of the small subunit ribosomal RNA gene of the mitochondrion (12S rDNA).
Results: Three of the examined dogs (1.8%) were found to be infected with only D. immitis, one (0.6%) with D. repens and three (1.8%) with both parasites. Besides, 10 out of 161 dogs (6.2%) were found infected with Wolbachia sp. Finaly, the seroprevalence of dirofilariosis in the examined dogs was found to be 3.7% (6/161).
Conclusion: Although dirofilariosis is not a serious problem in the region, the stray dogs still continue to be a source of infection. |
| first_indexed | 2024-12-12T05:34:37Z |
| format | Article |
| id | doaj.art-c69e1387ef124ceb856cc4399fd25066 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-12T05:34:37Z |
| publishDate | 2016-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-c69e1387ef124ceb856cc4399fd250662022-12-22T00:36:12ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712016-10-01104285Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in TurkeySami Simsek0Ayse Turkan Ciftci1Department of Parasitology, Faculty of Veterinary Medicine University of Firat, Elazig, TurkeyParasitology and Bee Diseases Laboratory, Veterinary Control and Research Institute, Elazig, TurkeyBackground: Dirofilaria immitis and Dirofilaria repens are the most common species of filarial nematodes described in the dogs. A single-step multiplex PCR was applied to detect and differentiate simultaneously and unequivocally D. immitis and D. repens on DNA extracted from canine peripheral blood and besides to detect the seroprevalance of D. immitis by ELISA in Elazig Province, Turkey. A PCR detection of the Wolbachia, which plays an important role in D. immitis biology and contributes to the inflammatory pathology of the heartworm, was also applied for the first time in Turkey. Methods: A total of 161 whole blood and sera samples were collected from stray dogs and stored at -20 °C until used. After DNA extraction, all samples were processed with Dirofilaria primers by multiplex-PCR and Wolbachia primers by conventional PCR besides ELISA for serology. The amplification was performed using a set of primers designed on a portion of the small subunit ribosomal RNA gene of the mitochondrion (12S rDNA). Results: Three of the examined dogs (1.8%) were found to be infected with only D. immitis, one (0.6%) with D. repens and three (1.8%) with both parasites. Besides, 10 out of 161 dogs (6.2%) were found infected with Wolbachia sp. Finaly, the seroprevalence of dirofilariosis in the examined dogs was found to be 3.7% (6/161). Conclusion: Although dirofilariosis is not a serious problem in the region, the stray dogs still continue to be a source of infection.https://jad.tums.ac.ir/index.php/jad/article/view/324Dirofilaria immitisDirofilaria repensWolbachiaMultiplex-PCRELISA |
| spellingShingle | Sami Simsek Ayse Turkan Ciftci Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey Journal of Arthropod-Borne Diseases Dirofilaria immitis Dirofilaria repens Wolbachia Multiplex-PCR ELISA |
| title | Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey |
| title_full | Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey |
| title_fullStr | Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey |
| title_full_unstemmed | Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey |
| title_short | Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey |
| title_sort | serological and molecular detection of dirofilaria species in stray dogs and investigation of wolbachia dna by pcr in turkey |
| topic | Dirofilaria immitis Dirofilaria repens Wolbachia Multiplex-PCR ELISA |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/324 |
| work_keys_str_mv | AT samisimsek serologicalandmoleculardetectionofdirofilariaspeciesinstraydogsandinvestigationofwolbachiadnabypcrinturkey AT ayseturkanciftci serologicalandmoleculardetectionofdirofilariaspeciesinstraydogsandinvestigationofwolbachiadnabypcrinturkey |