Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-grouting

The acclimation of sulfate-reducing bacteria (SRB) was performed to improve its adaptation in sulfate-rich systems with high sodium sulfate as a solution for disposing of soil salinization. The effects of pH, temperature, and carbon–sulfur ratio (CSR) on the activity and reduction efficiency (RE) of...

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Main Authors: Fan Wang, Shuquan Peng, Ling Fan, Yang Li
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Alexandria Engineering Journal
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1110016822002198
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author Fan Wang
Shuquan Peng
Ling Fan
Yang Li
author_facet Fan Wang
Shuquan Peng
Ling Fan
Yang Li
author_sort Fan Wang
collection DOAJ
description The acclimation of sulfate-reducing bacteria (SRB) was performed to improve its adaptation in sulfate-rich systems with high sodium sulfate as a solution for disposing of soil salinization. The effects of pH, temperature, and carbon–sulfur ratio (CSR) on the activity and reduction efficiency (RE) of SRB were analyzed, and the influence of grouting times (GTs) on RE was studied. The impact of different sulfate-rich systems (i.e., sulfate-rich solution and soil) with different salinity on the RE of SRB was investigated. Results show that the optimum temperature and pH for the best activity and RE of SRB were 30°C and 7.0, respectively. The multiple acclimations for SRB significantly improved the activity and RE of SRB at high CSR. The RE of SRB decreased with increasing salinity since high salinity could inhibit SRB activity. The maximum RE of SRB-200 in sulfate-rich soils with 2% salinity is 40%, which may be due to the significant salinity tolerance degree of SRB-200, which is higher than SRB-150 and SRB-175. In addition, the RE of SRB in sodium sulfate solution was higher than that in sulfate-rich soil due to the soil having a complex skeletal structure. The RE of SRB significantly increased after low GTs (<5) and then remained constant after high GTs (>5). The optimal GTs obtained by SRB-150, SRB-175 and SRB-200 were 4, 4 and 5, respectively, corresponding to the final cumulative REs of 37.2%, 45.2% and 55.2%, respectively. The SEM results shows the formation of sulfides verified the reliability of SRB reduction in sulfate-rich systems. All results provide an important reference for the effective utilization of SRB in sulfate-rich systems.
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spelling doaj.art-c69fa4ecc477470ebd232acf96fe54c72022-12-23T04:38:10ZengElsevierAlexandria Engineering Journal1110-01682022-12-016112999310005Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-groutingFan Wang0Shuquan Peng1Ling Fan2Yang Li3School of Civil Engineering, Central South University, Changsha, Hunan Province 410075, PR ChinaSchool of Resources and Safety Engineering, Central South University, Changsha, Hunan Province 410083, PR China; Corresponding author.School of Resources and Safety Engineering, Central South University, Changsha, Hunan Province 410083, PR ChinaSchool of Resource &amp; Environment and Safety Engineering, Hunan University of Science and Technology Xiangtan, Hunan 411021, PR ChinaThe acclimation of sulfate-reducing bacteria (SRB) was performed to improve its adaptation in sulfate-rich systems with high sodium sulfate as a solution for disposing of soil salinization. The effects of pH, temperature, and carbon–sulfur ratio (CSR) on the activity and reduction efficiency (RE) of SRB were analyzed, and the influence of grouting times (GTs) on RE was studied. The impact of different sulfate-rich systems (i.e., sulfate-rich solution and soil) with different salinity on the RE of SRB was investigated. Results show that the optimum temperature and pH for the best activity and RE of SRB were 30°C and 7.0, respectively. The multiple acclimations for SRB significantly improved the activity and RE of SRB at high CSR. The RE of SRB decreased with increasing salinity since high salinity could inhibit SRB activity. The maximum RE of SRB-200 in sulfate-rich soils with 2% salinity is 40%, which may be due to the significant salinity tolerance degree of SRB-200, which is higher than SRB-150 and SRB-175. In addition, the RE of SRB in sodium sulfate solution was higher than that in sulfate-rich soil due to the soil having a complex skeletal structure. The RE of SRB significantly increased after low GTs (<5) and then remained constant after high GTs (>5). The optimal GTs obtained by SRB-150, SRB-175 and SRB-200 were 4, 4 and 5, respectively, corresponding to the final cumulative REs of 37.2%, 45.2% and 55.2%, respectively. The SEM results shows the formation of sulfides verified the reliability of SRB reduction in sulfate-rich systems. All results provide an important reference for the effective utilization of SRB in sulfate-rich systems.http://www.sciencedirect.com/science/article/pii/S1110016822002198Sulfate reducing bacteriaReduction efficiencySoil salinizationAcclimationSulfate-rich systems
spellingShingle Fan Wang
Shuquan Peng
Ling Fan
Yang Li
Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-grouting
Alexandria Engineering Journal
Sulfate reducing bacteria
Reduction efficiency
Soil salinization
Acclimation
Sulfate-rich systems
title Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-grouting
title_full Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-grouting
title_fullStr Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-grouting
title_full_unstemmed Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-grouting
title_short Improved sulfate reduction efficiency of sulfate-reducing bacteria in sulfate-rich systems by acclimatization and multiple-grouting
title_sort improved sulfate reduction efficiency of sulfate reducing bacteria in sulfate rich systems by acclimatization and multiple grouting
topic Sulfate reducing bacteria
Reduction efficiency
Soil salinization
Acclimation
Sulfate-rich systems
url http://www.sciencedirect.com/science/article/pii/S1110016822002198
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