Characterization of envelope (E) protein of dengue virus Indonesia isolate
Dengue virus is a human pathogen that has as an increasingly important public health threat. These virus have four serotypes (DEN-1, DEN-2, DEN-3, DEN-4). There is no specific treatment for any pathogenic dengue virus and there are few vaccines available. This study was carry out to E protein charac...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Indonesian Biological Society
2000-06-01
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| Series: | Journal of Biological Researches |
| Subjects: | |
| Online Access: | http://berkalahayati.org/journal/d317bdcf |
| Summary: | Dengue virus is a human pathogen that has as an increasingly important public health threat. These virus have four serotypes (DEN-1, DEN-2, DEN-3, DEN-4). There is no specific treatment for any pathogenic dengue virus and there are few vaccines available. This study was carry out to E protein characterization from dengue virus infected C5/36 cells for vaccine candidate. We found that E protein induced antibody response. The data of the reactivity on the nitrocellulose membrane between E protein against polyclonal and monoclonal antibodies on western blot analysis, shown that the E protein whisc was once of dengue virus protein has immunogenic. The Indonesia isolate had no difference with the Phillipine isolate. The result suggested that all of the E protein dengue virus strain have peptides overlapping. Also promising for he production of immunogenic particulate proteins, especially DEN protein for the four strains. |
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| ISSN: | 0852-6834 2337-389X |