Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway
Pulmonary arterial hypertension (PAH) is an incurable disease with high mortality. Chemerin has been found to be associated with pulmonary hypertension (PH). However, the specific role of chemerin in mediating PH development remains unclear. This study aimed to elucidate the regulatory effects and t...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.767705/full |
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| author | Linqian Peng Linqian Peng Yunwei Chen Yunwei Chen Yan Li Yan Li Panpan Feng Yan Zheng Yan Zheng Yongjie Dong Yongjie Dong Yunjing Yang Ruiyu Wang Ruiyu Wang Ailing Li Jianghong Yan Feifei Shang Ping Tang Dewei Chen Yuqi Gao Wei Huang |
| author_facet | Linqian Peng Linqian Peng Yunwei Chen Yunwei Chen Yan Li Yan Li Panpan Feng Yan Zheng Yan Zheng Yongjie Dong Yongjie Dong Yunjing Yang Ruiyu Wang Ruiyu Wang Ailing Li Jianghong Yan Feifei Shang Ping Tang Dewei Chen Yuqi Gao Wei Huang |
| author_sort | Linqian Peng |
| collection | DOAJ |
| description | Pulmonary arterial hypertension (PAH) is an incurable disease with high mortality. Chemerin has been found to be associated with pulmonary hypertension (PH). However, the specific role of chemerin in mediating PH development remains unclear. This study aimed to elucidate the regulatory effects and the underlying mechanism of chemerin on PH and to investigate the expression levels of chemerin protein in plasma in PAH patients. In vivo, two animal models of PH were established in rats by monocrotaline (MCT) injection and hypoxia. We found that the expression levels of chemerin and its receptor, chemokine-like receptor 1 (CMKLR1), were significantly upregulated in the lungs of PH rats. Primary cultured pulmonary arterial smooth muscle cells [(PASMCs) (isolated from pulmonary arteries of normal healthy rats)] were exposed to hypoxia or treated with recombinant human chemerin, we found that CMKLR1 expression was upregulated in PASMCs in response to hypoxia or chemerin stimulation, whereas the exogenous chemerin significantly promoted the migration and proliferation of PASMCs. Notably, the regulatory effects of chemerin on PASMCs were blunted by PD98059 (a selective ERK1/2 inhibitor). Using enzyme linked immunosorbent assay (ELISA), we found that the protein level of chemerin was also markedly increased in plasma from idiopathic pulmonary arterial hypertension (IPAH) patients compared to that from healthy controls. Moreover, the diagnostic value of chemerin expression in IPAH patients was determined through receiver operating characteristic (ROC) curve analysis and the result revealed that area under ROC curve (AUC) for plasma chemerin was 0.949. Taken together, these results suggest that chemerin exacerbates PH progression by promoting the proliferation and migration of PASMCs via the ERK1/2 signaling pathway, and chemerin is associated with pulmonary hypertension. |
| first_indexed | 2024-12-11T15:15:42Z |
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| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T15:15:42Z |
| publishDate | 2022-03-01 |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-c6a07219afc24a6eadd3e939fb1ae2922022-12-22T01:00:35ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-03-011310.3389/fphar.2022.767705767705Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling PathwayLinqian Peng0Linqian Peng1Yunwei Chen2Yunwei Chen3Yan Li4Yan Li5Panpan Feng6Yan Zheng7Yan Zheng8Yongjie Dong9Yongjie Dong10Yunjing Yang11Ruiyu Wang12Ruiyu Wang13Ailing Li14Jianghong Yan15Feifei Shang16Ping Tang17Dewei Chen18Yuqi Gao19Wei Huang20Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaInstitute of Life Science, Chongqing Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Medicine and Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University, Chongqing, ChinaInstitute of Medicine and Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University, Chongqing, ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaPulmonary arterial hypertension (PAH) is an incurable disease with high mortality. Chemerin has been found to be associated with pulmonary hypertension (PH). However, the specific role of chemerin in mediating PH development remains unclear. This study aimed to elucidate the regulatory effects and the underlying mechanism of chemerin on PH and to investigate the expression levels of chemerin protein in plasma in PAH patients. In vivo, two animal models of PH were established in rats by monocrotaline (MCT) injection and hypoxia. We found that the expression levels of chemerin and its receptor, chemokine-like receptor 1 (CMKLR1), were significantly upregulated in the lungs of PH rats. Primary cultured pulmonary arterial smooth muscle cells [(PASMCs) (isolated from pulmonary arteries of normal healthy rats)] were exposed to hypoxia or treated with recombinant human chemerin, we found that CMKLR1 expression was upregulated in PASMCs in response to hypoxia or chemerin stimulation, whereas the exogenous chemerin significantly promoted the migration and proliferation of PASMCs. Notably, the regulatory effects of chemerin on PASMCs were blunted by PD98059 (a selective ERK1/2 inhibitor). Using enzyme linked immunosorbent assay (ELISA), we found that the protein level of chemerin was also markedly increased in plasma from idiopathic pulmonary arterial hypertension (IPAH) patients compared to that from healthy controls. Moreover, the diagnostic value of chemerin expression in IPAH patients was determined through receiver operating characteristic (ROC) curve analysis and the result revealed that area under ROC curve (AUC) for plasma chemerin was 0.949. Taken together, these results suggest that chemerin exacerbates PH progression by promoting the proliferation and migration of PASMCs via the ERK1/2 signaling pathway, and chemerin is associated with pulmonary hypertension.https://www.frontiersin.org/articles/10.3389/fphar.2022.767705/fullchemerinchemokine-like receptor 1pulmonary arterial hypertensionpulmonary arterial smooth muscle cellsERK1/2 |
| spellingShingle | Linqian Peng Linqian Peng Yunwei Chen Yunwei Chen Yan Li Yan Li Panpan Feng Yan Zheng Yan Zheng Yongjie Dong Yongjie Dong Yunjing Yang Ruiyu Wang Ruiyu Wang Ailing Li Jianghong Yan Feifei Shang Ping Tang Dewei Chen Yuqi Gao Wei Huang Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway Frontiers in Pharmacology chemerin chemokine-like receptor 1 pulmonary arterial hypertension pulmonary arterial smooth muscle cells ERK1/2 |
| title | Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway |
| title_full | Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway |
| title_fullStr | Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway |
| title_full_unstemmed | Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway |
| title_short | Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway |
| title_sort | chemerin regulates the proliferation and migration of pulmonary arterial smooth muscle cells via the erk1 2 signaling pathway |
| topic | chemerin chemokine-like receptor 1 pulmonary arterial hypertension pulmonary arterial smooth muscle cells ERK1/2 |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.767705/full |
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