Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishes
Hysterothylacium spp Larvae encysted within the mesenteries, peritoneum, and viscera, which is the perfect place to complete their life cycle. More than 4123 larval stages were isolated from 595 Saurida tumbil and Platycephalus indicus fish. This is a simple fact that these two species are feeding g...
| Główni autorzy: | , , , |
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| Format: | Artykuł |
| Język: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-10-01
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| Seria: | Iraqi Journal of Veterinary Sciences |
| Hasła przedmiotowe: | |
| Dostęp online: | https://www.vetmedmosul.com/article_180428_10c6f2e5f7624b674367e0ec7bf26f2a.pdf |
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| author | Majid Bannai Muna M. Jori Fawzi M. Alkhwaja Shokoofeh Shamsi |
| author_facet | Majid Bannai Muna M. Jori Fawzi M. Alkhwaja Shokoofeh Shamsi |
| author_sort | Majid Bannai |
| collection | DOAJ |
| description | Hysterothylacium spp Larvae encysted within the mesenteries, peritoneum, and viscera, which is the perfect place to complete their life cycle. More than 4123 larval stages were isolated from 595 Saurida tumbil and Platycephalus indicus fish. This is a simple fact that these two species are feeding greedily. The prevalence of infection was 95.3- 100 %. Internal transcribed spacers (ITS) gene of nuclear rDNA (rDNA) were amplified by conventional PCR, using the primer sets NC5/NC2. The ITS sequences determined were compared using the algorithm BLASTn with those available in the National Center for Biotechnology Information (NCBI) database. Multiple sequence alignments (MSA) were performed by ClustalW. The result showed that there are 22 distinct taxa within the genus Hysterothylacium, followed by 4 common ancestors, with 6 clades grouped. The study showed Sequence heterogeneity in the ITS gene within Hysterothylacium spp. of nematode parasite collected from Saurida tumbil and Platycephalus indicus fish. Moreover, genetic homogeneity between the population of the Arabian Gulf and other regions seemed different through the high levels of gene flow observed somewhat in this qualitative diversity. The most important of which may be the impact of climate. |
| first_indexed | 2024-03-11T20:40:43Z |
| format | Article |
| id | doaj.art-c6a1300132ba412f808f852d68c83e15 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-03-11T20:40:43Z |
| publishDate | 2023-10-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-c6a1300132ba412f808f852d68c83e152023-10-02T03:07:56ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-10-0137478979410.33899/ijvs.2023.137130.2641180428Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishesMajid Bannai0Muna M. Jori1Fawzi M. Alkhwaja2Shokoofeh Shamsi3Department of Marine Vertebrate, Marine Science Center, University of Basrah, Basrah, IraqDepartments of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Marine Vertebrate, Marine Science Center, University of Basrah, Basrah, IraqSchool of Animal and Veterinary Sciences, Charles Sturt University, New South Wales, AustraliaHysterothylacium spp Larvae encysted within the mesenteries, peritoneum, and viscera, which is the perfect place to complete their life cycle. More than 4123 larval stages were isolated from 595 Saurida tumbil and Platycephalus indicus fish. This is a simple fact that these two species are feeding greedily. The prevalence of infection was 95.3- 100 %. Internal transcribed spacers (ITS) gene of nuclear rDNA (rDNA) were amplified by conventional PCR, using the primer sets NC5/NC2. The ITS sequences determined were compared using the algorithm BLASTn with those available in the National Center for Biotechnology Information (NCBI) database. Multiple sequence alignments (MSA) were performed by ClustalW. The result showed that there are 22 distinct taxa within the genus Hysterothylacium, followed by 4 common ancestors, with 6 clades grouped. The study showed Sequence heterogeneity in the ITS gene within Hysterothylacium spp. of nematode parasite collected from Saurida tumbil and Platycephalus indicus fish. Moreover, genetic homogeneity between the population of the Arabian Gulf and other regions seemed different through the high levels of gene flow observed somewhat in this qualitative diversity. The most important of which may be the impact of climate.https://www.vetmedmosul.com/article_180428_10c6f2e5f7624b674367e0ec7bf26f2a.pdfhysterothylaciumanisakidssaurida tumbilplatycephalus indicusiraq |
| spellingShingle | Majid Bannai Muna M. Jori Fawzi M. Alkhwaja Shokoofeh Shamsi Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishes Iraqi Journal of Veterinary Sciences hysterothylacium anisakids saurida tumbil platycephalus indicus iraq |
| title | Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishes |
| title_full | Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishes |
| title_fullStr | Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishes |
| title_full_unstemmed | Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishes |
| title_short | Infection and genetic detection of Hysterothylacium spp (Ascaridida: Raphidascaridinae) infesting Saurida tumbil (Bloch, 1795) and Platycephalus indicus (Linnaeus, 1758) fishes, Iraqi marine water fishes |
| title_sort | infection and genetic detection of hysterothylacium spp ascaridida raphidascaridinae infesting saurida tumbil bloch 1795 and platycephalus indicus linnaeus 1758 fishes iraqi marine water fishes |
| topic | hysterothylacium anisakids saurida tumbil platycephalus indicus iraq |
| url | https://www.vetmedmosul.com/article_180428_10c6f2e5f7624b674367e0ec7bf26f2a.pdf |
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