The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell Arteritis
Purpose: To determine the appropriate number of histopathological cross-sections that are required for a conclusive diagnosis of giant cell arteritis (GCA). Methods: In this cross-sectional study, the number of sections per slide for paraffin-embedded blocks for 100 randomly selected cases wher...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Knowledge E
2021-01-01
|
| Series: | Journal of Ophthalmic & Vision Research |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/jovr.v16i1.8253 |
| _version_ | 1797990951419904000 |
|---|---|
| author | Roshanak Ali-Akbar Navahi Samira Chaibakhsh Sayyed Amirpooya Alemzadeh Kaveh Abri Aghdam |
| author_facet | Roshanak Ali-Akbar Navahi Samira Chaibakhsh Sayyed Amirpooya Alemzadeh Kaveh Abri Aghdam |
| author_sort | Roshanak Ali-Akbar Navahi |
| collection | DOAJ |
| description | Purpose: To determine the appropriate number of histopathological cross-sections that are required for a conclusive diagnosis of giant cell arteritis (GCA).
Methods: In this cross-sectional study, the number of sections per slide for paraffin-embedded blocks for 100 randomly selected cases where GCA was suspected and those for negative temporal artery biopsies (TABs) were compared with the number of cross-sections per specimen for eight positive-TABs. All aforementioned examinations were conducted at our center from 2012 to 2016. Then, negative-TABs were retrieved and re-evaluated using light microscopy considering the histopathological findings of GCA.
Results: Ninety-five paraffin blocks were retrieved. The original mean biopsy length was 15.39
±
7.56 mm. Comparison of the mean number of cross-sections per specimen for both the positive- and negative-TABs (9.25
±
3.37 and 9.53
±
2.46) showed that 9.87
±
2.77 [95% confidence intervals (CI)] cross-sections per specimen were sufficient for a precise GCA diagnosis. There was no statistically significant difference in the mean biopsy length (P = 0.142) among the eight positive-TABs. Similarly, no significant difference was observed in the number of cross-sections per specimen (P = 0.990) for positive-TABs compared to those for the negative-TABs. After the retrieval of negative-TABs, the mean number of total pre- and post-retrieval cross-sections per specimen was 17.66
±
4.43. Among all retrieved specimens, only one case (0.01%) showed the histopathological features of healed arteritis.
Conclusion: Positive-TABs did not reveal more histological cross-sections than the negative ones and increasing the number of cross-sections did not enhance the accuracy of TAB. |
| first_indexed | 2024-04-11T08:44:40Z |
| format | Article |
| id | doaj.art-c6a1a380b1484eed8b0cd8c09432fbd6 |
| institution | Directory Open Access Journal |
| issn | 2008-322X |
| language | English |
| last_indexed | 2024-04-11T08:44:40Z |
| publishDate | 2021-01-01 |
| publisher | Knowledge E |
| record_format | Article |
| series | Journal of Ophthalmic & Vision Research |
| spelling | doaj.art-c6a1a380b1484eed8b0cd8c09432fbd62022-12-22T04:34:00ZengKnowledge EJournal of Ophthalmic & Vision Research2008-322X2021-01-0116778310.18502/jovr.v16i1.8253jovr.v16i1.8253The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell ArteritisRoshanak Ali-Akbar Navahi0Samira Chaibakhsh1Sayyed Amirpooya Alemzadeh2Kaveh Abri Aghdam3 Eye Research Center, The Five Senses Institute, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran Eye Research Center, The Five Senses Institute, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran Eye Research Center, The Five Senses Institute, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran Eye Research Center, The Five Senses Institute, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, IranPurpose: To determine the appropriate number of histopathological cross-sections that are required for a conclusive diagnosis of giant cell arteritis (GCA). Methods: In this cross-sectional study, the number of sections per slide for paraffin-embedded blocks for 100 randomly selected cases where GCA was suspected and those for negative temporal artery biopsies (TABs) were compared with the number of cross-sections per specimen for eight positive-TABs. All aforementioned examinations were conducted at our center from 2012 to 2016. Then, negative-TABs were retrieved and re-evaluated using light microscopy considering the histopathological findings of GCA. Results: Ninety-five paraffin blocks were retrieved. The original mean biopsy length was 15.39 ± 7.56 mm. Comparison of the mean number of cross-sections per specimen for both the positive- and negative-TABs (9.25 ± 3.37 and 9.53 ± 2.46) showed that 9.87 ± 2.77 [95% confidence intervals (CI)] cross-sections per specimen were sufficient for a precise GCA diagnosis. There was no statistically significant difference in the mean biopsy length (P = 0.142) among the eight positive-TABs. Similarly, no significant difference was observed in the number of cross-sections per specimen (P = 0.990) for positive-TABs compared to those for the negative-TABs. After the retrieval of negative-TABs, the mean number of total pre- and post-retrieval cross-sections per specimen was 17.66 ± 4.43. Among all retrieved specimens, only one case (0.01%) showed the histopathological features of healed arteritis. Conclusion: Positive-TABs did not reveal more histological cross-sections than the negative ones and increasing the number of cross-sections did not enhance the accuracy of TAB.https://doi.org/10.18502/jovr.v16i1.8253giant cell arteritishistopathology cross-sectionstemporal artery biopsy |
| spellingShingle | Roshanak Ali-Akbar Navahi Samira Chaibakhsh Sayyed Amirpooya Alemzadeh Kaveh Abri Aghdam The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell Arteritis Journal of Ophthalmic & Vision Research giant cell arteritis histopathology cross-sections temporal artery biopsy |
| title | The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell Arteritis |
| title_full | The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell Arteritis |
| title_fullStr | The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell Arteritis |
| title_full_unstemmed | The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell Arteritis |
| title_short | The Adequate Number of Histopathology Cross-sections of Temporal Artery Biopsy in Establishing the Diagnosis of Giant Cell Arteritis |
| title_sort | adequate number of histopathology cross sections of temporal artery biopsy in establishing the diagnosis of giant cell arteritis |
| topic | giant cell arteritis histopathology cross-sections temporal artery biopsy |
| url | https://doi.org/10.18502/jovr.v16i1.8253 |
| work_keys_str_mv | AT roshanakaliakbarnavahi theadequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis AT samirachaibakhsh theadequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis AT sayyedamirpooyaalemzadeh theadequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis AT kavehabriaghdam theadequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis AT roshanakaliakbarnavahi adequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis AT samirachaibakhsh adequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis AT sayyedamirpooyaalemzadeh adequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis AT kavehabriaghdam adequatenumberofhistopathologycrosssectionsoftemporalarterybiopsyinestablishingthediagnosisofgiantcellarteritis |