Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean Oil
In recent years, synthetic antioxidants that are widely used in foods have been shown to cause detrimental health effects, and there has been growing interest in antioxidants realised from natural plant extracts. In this study, we investigate the potential effects of natural antioxidant components e...
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/9/2865 |
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| author | Xiuqiong Huang Wei Gao Xuan Yun Zhixing Qing Jianguo Zeng |
| author_facet | Xiuqiong Huang Wei Gao Xuan Yun Zhixing Qing Jianguo Zeng |
| author_sort | Xiuqiong Huang |
| collection | DOAJ |
| description | In recent years, synthetic antioxidants that are widely used in foods have been shown to cause detrimental health effects, and there has been growing interest in antioxidants realised from natural plant extracts. In this study, we investigate the potential effects of natural antioxidant components extracted from the forage plant marigold on the oxidative stability of soybean oil. First, HPLC-Q-TOF-MS/MS was used with 1,1-diphenyl-2-picrylhydrazyl (DPPH) to screen and identify potential antioxidant components in marigold. Four main antioxidant components were identified, including quercetagetin-7-<i>O</i>-glucoside (1), quercetagetin (2), quercetin (3) and patuletin (4). Among them, quercetagetin (QG) exhibited the highest content and the strongest DPPH radical scavenging activity and effectively inhibited the production of oxidation products in soybean oil during accelerated oxidation, as indicated by reductions in the peroxide value (PV) and acid value (AV). Then, the fatty acids and volatile compounds of soybean oil were determined with gas chromatography–mass spectrometry (GC-MS) and headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS). A total of 108 volatile components, including 16 alcohols, 23 aldehydes, 25 ketones, 4 acids, 15 esters, 18 hydrocarbons, and 7 other compounds, were identified. QG significantly reduced the content and number of aldehydes and ketones, whereas the formation of acids and hydrocarbons was completely prevented. In addition, the fatty acid analysis demonstrated that QG significantly inhibited oxidation of unsaturated fatty acids. Consequently, QG was identified as a potential, new natural antioxidant that is believed to be safe, effective and economical, and it may have potential for use in plant extracts feed additives. |
| first_indexed | 2024-03-10T03:54:01Z |
| format | Article |
| id | doaj.art-c6a34699e0a344afb27396a2726dbe40 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:54:01Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c6a34699e0a344afb27396a2726dbe402023-11-23T08:50:48ZengMDPI AGMolecules1420-30492022-04-01279286510.3390/molecules27092865Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean OilXiuqiong Huang0Wei Gao1Xuan Yun2Zhixing Qing3Jianguo Zeng4Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaChenguang Biotechnology Company Limited, Handan 056000, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaIn recent years, synthetic antioxidants that are widely used in foods have been shown to cause detrimental health effects, and there has been growing interest in antioxidants realised from natural plant extracts. In this study, we investigate the potential effects of natural antioxidant components extracted from the forage plant marigold on the oxidative stability of soybean oil. First, HPLC-Q-TOF-MS/MS was used with 1,1-diphenyl-2-picrylhydrazyl (DPPH) to screen and identify potential antioxidant components in marigold. Four main antioxidant components were identified, including quercetagetin-7-<i>O</i>-glucoside (1), quercetagetin (2), quercetin (3) and patuletin (4). Among them, quercetagetin (QG) exhibited the highest content and the strongest DPPH radical scavenging activity and effectively inhibited the production of oxidation products in soybean oil during accelerated oxidation, as indicated by reductions in the peroxide value (PV) and acid value (AV). Then, the fatty acids and volatile compounds of soybean oil were determined with gas chromatography–mass spectrometry (GC-MS) and headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS). A total of 108 volatile components, including 16 alcohols, 23 aldehydes, 25 ketones, 4 acids, 15 esters, 18 hydrocarbons, and 7 other compounds, were identified. QG significantly reduced the content and number of aldehydes and ketones, whereas the formation of acids and hydrocarbons was completely prevented. In addition, the fatty acid analysis demonstrated that QG significantly inhibited oxidation of unsaturated fatty acids. Consequently, QG was identified as a potential, new natural antioxidant that is believed to be safe, effective and economical, and it may have potential for use in plant extracts feed additives.https://www.mdpi.com/1420-3049/27/9/2865<i>Tagetes erecta</i> L.quercetagetinDPPH-HPLC-Q-TOF/MSHS-SPME-GC-MSoxidative stability |
| spellingShingle | Xiuqiong Huang Wei Gao Xuan Yun Zhixing Qing Jianguo Zeng Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean Oil Molecules <i>Tagetes erecta</i> L. quercetagetin DPPH-HPLC-Q-TOF/MS HS-SPME-GC-MS oxidative stability |
| title | Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean Oil |
| title_full | Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean Oil |
| title_fullStr | Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean Oil |
| title_full_unstemmed | Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean Oil |
| title_short | Effect of Natural Antioxidants from Marigolds (<i>Tagetes erecta</i> L.) on the Oxidative Stability of Soybean Oil |
| title_sort | effect of natural antioxidants from marigolds i tagetes erecta i l on the oxidative stability of soybean oil |
| topic | <i>Tagetes erecta</i> L. quercetagetin DPPH-HPLC-Q-TOF/MS HS-SPME-GC-MS oxidative stability |
| url | https://www.mdpi.com/1420-3049/27/9/2865 |
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