The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and Implantation
The 3D reconstruction of 100 μm- and 600 μm-thick fibrous poly-L/L-lactide scaffolds was performed by confocal laser scanning microscopy and supported by scanning electron microscopy and showed that the density of the fibers on the side adjacent to the electrode is higher, which can affect cell diff...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/20/4352 |
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| author | Timur Kh. Tenchurin Alla V. Rodina Vladimir P. Saprykin Lada V. Gorshkova Alexey A. Mikhutkin Roman A. Kamyshinsky Dmitry S. Yakovlev Alexander L. Vasiliev Sergey N. Chvalun Timofey E. Grigoriev |
| author_facet | Timur Kh. Tenchurin Alla V. Rodina Vladimir P. Saprykin Lada V. Gorshkova Alexey A. Mikhutkin Roman A. Kamyshinsky Dmitry S. Yakovlev Alexander L. Vasiliev Sergey N. Chvalun Timofey E. Grigoriev |
| author_sort | Timur Kh. Tenchurin |
| collection | DOAJ |
| description | The 3D reconstruction of 100 μm- and 600 μm-thick fibrous poly-L/L-lactide scaffolds was performed by confocal laser scanning microscopy and supported by scanning electron microscopy and showed that the density of the fibers on the side adjacent to the electrode is higher, which can affect cell diffusion, while the pore size is generally the same. Bone marrow mesenchymal stem cells cultured in a 600 μm-thick scaffold formed colonies and produced conditions for cell differentiation. An in vitro study of stem cells after 7 days revealed that cell proliferation and hepatocyte growth factor release in the 600 μm-thick scaffold were higher than in the 100 μm-thick scaffold. An in vivo study of scaffolds with and without stem cells implanted subcutaneously onto the backs of recipient mice was carried out to test their biodegradation and biocompatibility over a 0–3-week period. The cells seeded onto the 600 μm-thick scaffold promoted significant neovascularization in vivo. After 3 weeks, a significant number of donor cells persisted only on the inside of the 600 μm-thick scaffold. Thus, the use of bulkier matrices allows to prolong the effect of secretion of growth factors by stem cells during implantation. These 600 μm-thick scaffolds could potentially be utilized to repair and regenerate injuries with stem cell co-culture for vascularization of implant. |
| first_indexed | 2024-03-09T19:33:01Z |
| format | Article |
| id | doaj.art-c6a3ba097d1740688dc43de095081a6e |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T19:33:01Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-c6a3ba097d1740688dc43de095081a6e2023-11-24T02:07:46ZengMDPI AGPolymers2073-43602022-10-011420435210.3390/polym14204352The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and ImplantationTimur Kh. Tenchurin0Alla V. Rodina1Vladimir P. Saprykin2Lada V. Gorshkova3Alexey A. Mikhutkin4Roman A. Kamyshinsky5Dmitry S. Yakovlev6Alexander L. Vasiliev7Sergey N. Chvalun8Timofey E. Grigoriev9National Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaNational Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaNatural Sciences Department, Moscow Region State University, 105005 Moscow, RussiaNational Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaNational Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaNational Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaRussian Quantum Center, Skolkovo, 121205 Moscow, RussiaNational Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaNational Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaNational Research Centre “Kurchatov Institute”, 123098 Moscow, RussiaThe 3D reconstruction of 100 μm- and 600 μm-thick fibrous poly-L/L-lactide scaffolds was performed by confocal laser scanning microscopy and supported by scanning electron microscopy and showed that the density of the fibers on the side adjacent to the electrode is higher, which can affect cell diffusion, while the pore size is generally the same. Bone marrow mesenchymal stem cells cultured in a 600 μm-thick scaffold formed colonies and produced conditions for cell differentiation. An in vitro study of stem cells after 7 days revealed that cell proliferation and hepatocyte growth factor release in the 600 μm-thick scaffold were higher than in the 100 μm-thick scaffold. An in vivo study of scaffolds with and without stem cells implanted subcutaneously onto the backs of recipient mice was carried out to test their biodegradation and biocompatibility over a 0–3-week period. The cells seeded onto the 600 μm-thick scaffold promoted significant neovascularization in vivo. After 3 weeks, a significant number of donor cells persisted only on the inside of the 600 μm-thick scaffold. Thus, the use of bulkier matrices allows to prolong the effect of secretion of growth factors by stem cells during implantation. These 600 μm-thick scaffolds could potentially be utilized to repair and regenerate injuries with stem cell co-culture for vascularization of implant.https://www.mdpi.com/2073-4360/14/20/4352tissue engineeringfibrous scaffoldspolylactidemesenchymal stem cellsbone marrowmicroscopy |
| spellingShingle | Timur Kh. Tenchurin Alla V. Rodina Vladimir P. Saprykin Lada V. Gorshkova Alexey A. Mikhutkin Roman A. Kamyshinsky Dmitry S. Yakovlev Alexander L. Vasiliev Sergey N. Chvalun Timofey E. Grigoriev The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and Implantation Polymers tissue engineering fibrous scaffolds polylactide mesenchymal stem cells bone marrow microscopy |
| title | The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and Implantation |
| title_full | The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and Implantation |
| title_fullStr | The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and Implantation |
| title_full_unstemmed | The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and Implantation |
| title_short | The Performance of Nonwoven PLLA Scaffolds of Different Thickness for Stem Cells Seeding and Implantation |
| title_sort | performance of nonwoven plla scaffolds of different thickness for stem cells seeding and implantation |
| topic | tissue engineering fibrous scaffolds polylactide mesenchymal stem cells bone marrow microscopy |
| url | https://www.mdpi.com/2073-4360/14/20/4352 |
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