Assays to Monitor Autophagy in Saccharomyces cerevisiae
Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/6/3/23 |
| _version_ | 1797712493349437440 |
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| author | Raffaela Torggler Daniel Papinski Claudine Kraft |
| author_facet | Raffaela Torggler Daniel Papinski Claudine Kraft |
| author_sort | Raffaela Torggler |
| collection | DOAJ |
| description | Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is not yet completely understood. The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up on these studies, many homologs have been found in higher eukaryotes. Yeast remains a highly relevant model organism for studying autophagy, with a wide range of established methods to elucidate the molecular details of the autophagy pathway. In this review, we provide an overview of methods to study both selective and bulk autophagy, including intermediate steps in the yeast Saccharomyces cerevisiae. We compare different assays, discuss their advantages and limitations and list potential applications. |
| first_indexed | 2024-03-12T07:22:36Z |
| format | Article |
| id | doaj.art-c6a644a4d2a34dbdb4b839fa5df940fc |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T07:22:36Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-c6a644a4d2a34dbdb4b839fa5df940fc2023-09-02T22:20:31ZengMDPI AGCells2073-44092017-07-01632310.3390/cells6030023cells6030023Assays to Monitor Autophagy in Saccharomyces cerevisiaeRaffaela Torggler0Daniel Papinski1Claudine Kraft2Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), Dr.-Bohr-Gasse 9, 1030 Vienna, AustriaMax F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), Dr.-Bohr-Gasse 9, 1030 Vienna, AustriaMax F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), Dr.-Bohr-Gasse 9, 1030 Vienna, AustriaAutophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is not yet completely understood. The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up on these studies, many homologs have been found in higher eukaryotes. Yeast remains a highly relevant model organism for studying autophagy, with a wide range of established methods to elucidate the molecular details of the autophagy pathway. In this review, we provide an overview of methods to study both selective and bulk autophagy, including intermediate steps in the yeast Saccharomyces cerevisiae. We compare different assays, discuss their advantages and limitations and list potential applications.https://www.mdpi.com/2073-4409/6/3/23yeastbulk autophagyCvt pathwayselective autophagyPASautophagosomeautophagic bodyiPassM-Track |
| spellingShingle | Raffaela Torggler Daniel Papinski Claudine Kraft Assays to Monitor Autophagy in Saccharomyces cerevisiae Cells yeast bulk autophagy Cvt pathway selective autophagy PAS autophagosome autophagic body iPass M-Track |
| title | Assays to Monitor Autophagy in Saccharomyces cerevisiae |
| title_full | Assays to Monitor Autophagy in Saccharomyces cerevisiae |
| title_fullStr | Assays to Monitor Autophagy in Saccharomyces cerevisiae |
| title_full_unstemmed | Assays to Monitor Autophagy in Saccharomyces cerevisiae |
| title_short | Assays to Monitor Autophagy in Saccharomyces cerevisiae |
| title_sort | assays to monitor autophagy in saccharomyces cerevisiae |
| topic | yeast bulk autophagy Cvt pathway selective autophagy PAS autophagosome autophagic body iPass M-Track |
| url | https://www.mdpi.com/2073-4409/6/3/23 |
| work_keys_str_mv | AT raffaelatorggler assaystomonitorautophagyinsaccharomycescerevisiae AT danielpapinski assaystomonitorautophagyinsaccharomycescerevisiae AT claudinekraft assaystomonitorautophagyinsaccharomycescerevisiae |