iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy
Abstract Expansion microscopy (ExM) is a highly effective technique for super-resolution fluorescence microscopy that enables imaging of biological samples beyond the diffraction limit with conventional fluorescence microscopes. Despite the development of several enhanced protocols, ExM has not yet...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-43582-8 |
| _version_ | 1797414749050241024 |
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| author | Vincent Louvel Romuald Haase Olivier Mercey Marine H. Laporte Thibaut Eloy Étienne Baudrier Denis Fortun Dominique Soldati-Favre Virginie Hamel Paul Guichard |
| author_facet | Vincent Louvel Romuald Haase Olivier Mercey Marine H. Laporte Thibaut Eloy Étienne Baudrier Denis Fortun Dominique Soldati-Favre Virginie Hamel Paul Guichard |
| author_sort | Vincent Louvel |
| collection | DOAJ |
| description | Abstract Expansion microscopy (ExM) is a highly effective technique for super-resolution fluorescence microscopy that enables imaging of biological samples beyond the diffraction limit with conventional fluorescence microscopes. Despite the development of several enhanced protocols, ExM has not yet demonstrated the ability to achieve the precision of nanoscopy techniques such as Single Molecule Localization Microscopy (SMLM). Here, to address this limitation, we have developed an iterative ultrastructure expansion microscopy (iU-ExM) approach that achieves SMLM-level resolution. With iU-ExM, it is now possible to visualize the molecular architecture of gold-standard samples, such as the eight-fold symmetry of nuclear pores or the molecular organization of the conoid in Apicomplexa. With its wide-ranging applications, from isolated organelles to cells and tissue, iU-ExM opens new super-resolution avenues for scientists studying biological structures and functions. |
| first_indexed | 2024-03-09T05:38:03Z |
| format | Article |
| id | doaj.art-c6a6a3bb5cfb4d21a6b92a1f7f57c8cd |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-09T05:38:03Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c6a6a3bb5cfb4d21a6b92a1f7f57c8cd2023-12-03T12:27:32ZengNature PortfolioNature Communications2041-17232023-11-0114111810.1038/s41467-023-43582-8iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopyVincent Louvel0Romuald Haase1Olivier Mercey2Marine H. Laporte3Thibaut Eloy4Étienne Baudrier5Denis Fortun6Dominique Soldati-Favre7Virginie Hamel8Paul Guichard9Department of Molecular and Cellular Biology, University of GenevaDepartment of Microbiology and Molecular medicine, University of GenevaDepartment of Molecular and Cellular Biology, University of GenevaDepartment of Molecular and Cellular Biology, University of GenevaICube - UMR7357, CNRS, University of StrasbourgICube - UMR7357, CNRS, University of StrasbourgICube - UMR7357, CNRS, University of StrasbourgDepartment of Microbiology and Molecular medicine, University of GenevaDepartment of Molecular and Cellular Biology, University of GenevaDepartment of Molecular and Cellular Biology, University of GenevaAbstract Expansion microscopy (ExM) is a highly effective technique for super-resolution fluorescence microscopy that enables imaging of biological samples beyond the diffraction limit with conventional fluorescence microscopes. Despite the development of several enhanced protocols, ExM has not yet demonstrated the ability to achieve the precision of nanoscopy techniques such as Single Molecule Localization Microscopy (SMLM). Here, to address this limitation, we have developed an iterative ultrastructure expansion microscopy (iU-ExM) approach that achieves SMLM-level resolution. With iU-ExM, it is now possible to visualize the molecular architecture of gold-standard samples, such as the eight-fold symmetry of nuclear pores or the molecular organization of the conoid in Apicomplexa. With its wide-ranging applications, from isolated organelles to cells and tissue, iU-ExM opens new super-resolution avenues for scientists studying biological structures and functions.https://doi.org/10.1038/s41467-023-43582-8 |
| spellingShingle | Vincent Louvel Romuald Haase Olivier Mercey Marine H. Laporte Thibaut Eloy Étienne Baudrier Denis Fortun Dominique Soldati-Favre Virginie Hamel Paul Guichard iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy Nature Communications |
| title | iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy |
| title_full | iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy |
| title_fullStr | iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy |
| title_full_unstemmed | iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy |
| title_short | iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy |
| title_sort | iu exm nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy |
| url | https://doi.org/10.1038/s41467-023-43582-8 |
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