Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes
Rust fungi cause significant damage to wheat production worldwide. In order to mitigate disease impact and improve food security via durable resistance, it is important to understand the molecular basis of host–pathogen interactions. Despite a long history of research and high agricultural importanc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/2930 |
| _version_ | 1797264391879524352 |
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| author | Taras Nazarov Yan Liu Xianming Chen Deven R. See |
| author_facet | Taras Nazarov Yan Liu Xianming Chen Deven R. See |
| author_sort | Taras Nazarov |
| collection | DOAJ |
| description | Rust fungi cause significant damage to wheat production worldwide. In order to mitigate disease impact and improve food security via durable resistance, it is important to understand the molecular basis of host–pathogen interactions. Despite a long history of research and high agricultural importance, still little is known about the interactions between the stripe rust fungus and wheat host on the gene expression level. Here, we present analysis of the molecular interactions between a major wheat pathogen—<i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>)—in resistant and susceptible host backgrounds. Using plants with durable nonrace-specific resistance along with fully susceptible ones allowed us to show how gene expression patterns shift in compatible versus incompatible interactions. The pathogen showed significantly greater number and fold changes of overexpressed genes on the resistant host than the susceptible host. Stress-related pathways including MAPK, oxidation–reduction, osmotic stress, and stress granule formation were, almost exclusively, upregulated in the resistant host background, suggesting the requirement of the resistance-countermeasure mechanism facilitated by <i>Pst</i>. In contrast, the susceptible host background allowed for broad overrepresentation of the nutrient uptake pathways. This is the first study focused on the stripe rust pathogen–wheat interactions, on the whole transcriptome level, from the pathogen side. It lays a foundation for the better understanding of the resistant/susceptible hosts versus pathogenic fungus interaction in a broader sense. |
| first_indexed | 2024-04-25T00:28:10Z |
| format | Article |
| id | doaj.art-c6a6b695c3114900a3e3801f49987a73 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:28:10Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c6a6b695c3114900a3e3801f49987a732024-03-12T16:46:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255293010.3390/ijms25052930Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat GenotypesTaras Nazarov0Yan Liu1Xianming Chen2Deven R. See3Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USADepartment of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USADepartment of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USADepartment of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USARust fungi cause significant damage to wheat production worldwide. In order to mitigate disease impact and improve food security via durable resistance, it is important to understand the molecular basis of host–pathogen interactions. Despite a long history of research and high agricultural importance, still little is known about the interactions between the stripe rust fungus and wheat host on the gene expression level. Here, we present analysis of the molecular interactions between a major wheat pathogen—<i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>)—in resistant and susceptible host backgrounds. Using plants with durable nonrace-specific resistance along with fully susceptible ones allowed us to show how gene expression patterns shift in compatible versus incompatible interactions. The pathogen showed significantly greater number and fold changes of overexpressed genes on the resistant host than the susceptible host. Stress-related pathways including MAPK, oxidation–reduction, osmotic stress, and stress granule formation were, almost exclusively, upregulated in the resistant host background, suggesting the requirement of the resistance-countermeasure mechanism facilitated by <i>Pst</i>. In contrast, the susceptible host background allowed for broad overrepresentation of the nutrient uptake pathways. This is the first study focused on the stripe rust pathogen–wheat interactions, on the whole transcriptome level, from the pathogen side. It lays a foundation for the better understanding of the resistant/susceptible hosts versus pathogenic fungus interaction in a broader sense.https://www.mdpi.com/1422-0067/25/5/2930wheatstripe rusthost–pathogen interactionRNA-seqtranscriptome |
| spellingShingle | Taras Nazarov Yan Liu Xianming Chen Deven R. See Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes International Journal of Molecular Sciences wheat stripe rust host–pathogen interaction RNA-seq transcriptome |
| title | Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes |
| title_full | Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes |
| title_fullStr | Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes |
| title_full_unstemmed | Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes |
| title_short | Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes |
| title_sort | molecular mechanisms of the stripe rust interaction with resistant and susceptible wheat genotypes |
| topic | wheat stripe rust host–pathogen interaction RNA-seq transcriptome |
| url | https://www.mdpi.com/1422-0067/25/5/2930 |
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