Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching

Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching

Label-free homogeneous optical detection of low concentration of oligonucleotides using graphene oxide in complex solutions containing proteins remains difficult. We used a colloidal graphene oxide (GO) as a fluorescent probe quencher to detect microRNA-21 spiked-in cell culture medium, overcoming p...

Full description

Bibliographic Details
Main Authors: Florentin R. Nitu, Lorand Savu, Sorin Muraru, Ioan Stoian, Mariana Ionită
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Nanomaterials
Subjects:
graphene oxide
surfactants
fluorescence quenching
FRET
optical microRNA sensor
Online Access:https://www.mdpi.com/2079-4991/11/2/368

Internet

https://www.mdpi.com/2079-4991/11/2/368

Similar Items