Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications
Considering the high prevalence of cartilage-associated pathologies, low self-repair capacity and limitations of current repair techniques, tissue engineering (TE) strategies have emerged as a promising alternative in this field. Three-dimensional culture techniques have gained attention in recent y...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/3/292 |
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| author | David Sánchez-Porras Daniel Durand-Herrera Ana B. Paes Jesús Chato-Astrain Rik Verplancke Jan Vanfleteren José Darío Sánchez-López Óscar Darío García-García Fernando Campos Víctor Carriel |
| author_facet | David Sánchez-Porras Daniel Durand-Herrera Ana B. Paes Jesús Chato-Astrain Rik Verplancke Jan Vanfleteren José Darío Sánchez-López Óscar Darío García-García Fernando Campos Víctor Carriel |
| author_sort | David Sánchez-Porras |
| collection | DOAJ |
| description | Considering the high prevalence of cartilage-associated pathologies, low self-repair capacity and limitations of current repair techniques, tissue engineering (TE) strategies have emerged as a promising alternative in this field. Three-dimensional culture techniques have gained attention in recent years, showing their ability to provide the most biomimetic environment for the cells under culture conditions, enabling the cells to fabricate natural, 3D functional microtissues (MTs). In this sense, the aim of this study was to generate, characterize and compare scaffold-free human hyaline and elastic cartilage-derived MTs (HC-MTs and EC-MTs, respectively) under expansion (EM) and chondrogenic media (CM). MTs were generated by using agarose microchips and evaluated ex vivo for 28 days. The MTs generated were subjected to morphometric assessment and cell viability, metabolic activity and histological analyses. Results suggest that the use of CM improves the biomimicry of the MTs obtained in terms of morphology, viability and extracellular matrix (ECM) synthesis with respect to the use of EM. Moreover, the overall results indicate a faster and more sensitive response of the EC-derived cells to the use of CM as compared to HC chondrocytes. Finally, future preclinical in vivo studies are still needed to determine the potential clinical usefulness of these novel advanced therapy products. |
| first_indexed | 2024-03-10T13:16:48Z |
| format | Article |
| id | doaj.art-c6b22b35ff284251881638389ad7342e |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T13:16:48Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-c6b22b35ff284251881638389ad7342e2023-11-21T10:19:44ZengMDPI AGBiomedicines2227-90592021-03-019329210.3390/biomedicines9030292Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering ApplicationsDavid Sánchez-Porras0Daniel Durand-Herrera1Ana B. Paes2Jesús Chato-Astrain3Rik Verplancke4Jan Vanfleteren5José Darío Sánchez-López6Óscar Darío García-García7Fernando Campos8Víctor Carriel9Department of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, 18016 Granada, SpainDepartment of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, 18016 Granada, SpainMaster Program in Tissue Engineering and Advanced Therapies, International School for Postgraduate Studies, University of Granada, 18016 Granada, SpainDepartment of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, 18016 Granada, SpainCentre for Microsystems Technology (CMST), imec and Ghent University, 9052 Ghent, BelgiumCentre for Microsystems Technology (CMST), imec and Ghent University, 9052 Ghent, BelgiumDivision of Maxillofacial Surgery, University Hospital Complex of Granada, 18013 Granada, SpainDepartment of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, 18016 Granada, SpainDepartment of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, 18016 Granada, SpainDepartment of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, 18016 Granada, SpainConsidering the high prevalence of cartilage-associated pathologies, low self-repair capacity and limitations of current repair techniques, tissue engineering (TE) strategies have emerged as a promising alternative in this field. Three-dimensional culture techniques have gained attention in recent years, showing their ability to provide the most biomimetic environment for the cells under culture conditions, enabling the cells to fabricate natural, 3D functional microtissues (MTs). In this sense, the aim of this study was to generate, characterize and compare scaffold-free human hyaline and elastic cartilage-derived MTs (HC-MTs and EC-MTs, respectively) under expansion (EM) and chondrogenic media (CM). MTs were generated by using agarose microchips and evaluated ex vivo for 28 days. The MTs generated were subjected to morphometric assessment and cell viability, metabolic activity and histological analyses. Results suggest that the use of CM improves the biomimicry of the MTs obtained in terms of morphology, viability and extracellular matrix (ECM) synthesis with respect to the use of EM. Moreover, the overall results indicate a faster and more sensitive response of the EC-derived cells to the use of CM as compared to HC chondrocytes. Finally, future preclinical in vivo studies are still needed to determine the potential clinical usefulness of these novel advanced therapy products.https://www.mdpi.com/2227-9059/9/3/292microtissuestissue engineeringhuman hyaline chondrocyteshuman elastic chondrocytesextracellular matrixorganoids |
| spellingShingle | David Sánchez-Porras Daniel Durand-Herrera Ana B. Paes Jesús Chato-Astrain Rik Verplancke Jan Vanfleteren José Darío Sánchez-López Óscar Darío García-García Fernando Campos Víctor Carriel Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications Biomedicines microtissues tissue engineering human hyaline chondrocytes human elastic chondrocytes extracellular matrix organoids |
| title | Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications |
| title_full | Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications |
| title_fullStr | Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications |
| title_full_unstemmed | Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications |
| title_short | Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications |
| title_sort | ex vivo generation and characterization of human hyaline and elastic cartilaginous microtissues for tissue engineering applications |
| topic | microtissues tissue engineering human hyaline chondrocytes human elastic chondrocytes extracellular matrix organoids |
| url | https://www.mdpi.com/2227-9059/9/3/292 |
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