The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka
Traditional folk medicine in Sri Lanka is mostly based on plants and plant-derived products, however, many of these medicinal plant species are scientifically unexplored. Here, we evaluated the anti-inflammatory and antimicrobial potency of 28 different extracts prepared from seven popular medicinal...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/1894 |
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| author | Mayuri Napagoda Jana Gerstmeier Hannah Butschek Sudhara De Soyza Simona Pace Sybille Lorenz Mallique Qader Sanjeeva Witharana Ajith Nagahawatte Gaya Wijayaratne Aleš Svatoš Lalith Jayasinghe Andreas Koeberle Oliver Werz |
| author_facet | Mayuri Napagoda Jana Gerstmeier Hannah Butschek Sudhara De Soyza Simona Pace Sybille Lorenz Mallique Qader Sanjeeva Witharana Ajith Nagahawatte Gaya Wijayaratne Aleš Svatoš Lalith Jayasinghe Andreas Koeberle Oliver Werz |
| author_sort | Mayuri Napagoda |
| collection | DOAJ |
| description | Traditional folk medicine in Sri Lanka is mostly based on plants and plant-derived products, however, many of these medicinal plant species are scientifically unexplored. Here, we evaluated the anti-inflammatory and antimicrobial potency of 28 different extracts prepared from seven popular medicinal plant species employed in Sri Lanka. The extracts were subjected to cell-based and cell-free assays of 5-lipoxygenase (5-LO), microsomal prostaglandin E<sub>2</sub> synthase (mPGES)-1, and nitric oxide (NO) scavenging activity. Moreover, antibacterial and disinfectant activities were assessed. Characterization of secondary metabolites was achieved by gas chromatography coupled to mass spectrometric (GC-MS) analysis. <i>n</i>-Hexane- and dichloromethane-based extracts of <i>Garcinia cambogia</i> efficiently suppressed 5-LO activity in human neutrophils (IC<sub>50</sub> = 0.92 and 1.39 µg/mL), and potently inhibited isolated human 5-LO (IC<sub>50</sub> = 0.15 and 0.16 µg/mL) and mPGES-1 (IC<sub>50</sub> = 0.29 and 0.49 µg/mL). Lipophilic extracts of <i>Pothos scandens</i> displayed potent inhibition of mPGES-1 only. A methanolic extract of <i>Ophiorrhiza mungos</i> caused significant NO scavenging activity. The lipophilic extracts of <i>G. cambogia</i> exhibited prominent antibacterial and disinfectant activities, and GC-MS analysis revealed the presence of fatty acids, sesquiterpenes and other types of secondary metabolites. Together, our results suggest the prospective utilization of <i>G.</i> <i>cambogia</i> as disinfective agent with potent anti-inflammatory properties. |
| first_indexed | 2024-03-10T20:20:38Z |
| format | Article |
| id | doaj.art-c6b29798441c4263a0f856926a93780b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:20:38Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-c6b29798441c4263a0f856926a93780b2023-11-19T22:12:26ZengMDPI AGMolecules1420-30492020-04-01258189410.3390/molecules25081894The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri LankaMayuri Napagoda0Jana Gerstmeier1Hannah Butschek2Sudhara De Soyza3Simona Pace4Sybille Lorenz5Mallique Qader6Sanjeeva Witharana7Ajith Nagahawatte8Gaya Wijayaratne9Aleš Svatoš10Lalith Jayasinghe11Andreas Koeberle12Oliver Werz13Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, GermanyDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, GermanyDepartment of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, GermanyResearch Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, 07745 Jena, GermanyNational Institute of Fundamental Studies, Kandy 20000, Sri LankaFaculty of Engineering, Higher Colleges of Technology, PO Box 4793 Abu Dhabi, UAEDepartment of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaDepartment of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaResearch Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, 07745 Jena, GermanyNational Institute of Fundamental Studies, Kandy 20000, Sri LankaDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, GermanyDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, GermanyTraditional folk medicine in Sri Lanka is mostly based on plants and plant-derived products, however, many of these medicinal plant species are scientifically unexplored. Here, we evaluated the anti-inflammatory and antimicrobial potency of 28 different extracts prepared from seven popular medicinal plant species employed in Sri Lanka. The extracts were subjected to cell-based and cell-free assays of 5-lipoxygenase (5-LO), microsomal prostaglandin E<sub>2</sub> synthase (mPGES)-1, and nitric oxide (NO) scavenging activity. Moreover, antibacterial and disinfectant activities were assessed. Characterization of secondary metabolites was achieved by gas chromatography coupled to mass spectrometric (GC-MS) analysis. <i>n</i>-Hexane- and dichloromethane-based extracts of <i>Garcinia cambogia</i> efficiently suppressed 5-LO activity in human neutrophils (IC<sub>50</sub> = 0.92 and 1.39 µg/mL), and potently inhibited isolated human 5-LO (IC<sub>50</sub> = 0.15 and 0.16 µg/mL) and mPGES-1 (IC<sub>50</sub> = 0.29 and 0.49 µg/mL). Lipophilic extracts of <i>Pothos scandens</i> displayed potent inhibition of mPGES-1 only. A methanolic extract of <i>Ophiorrhiza mungos</i> caused significant NO scavenging activity. The lipophilic extracts of <i>G. cambogia</i> exhibited prominent antibacterial and disinfectant activities, and GC-MS analysis revealed the presence of fatty acids, sesquiterpenes and other types of secondary metabolites. Together, our results suggest the prospective utilization of <i>G.</i> <i>cambogia</i> as disinfective agent with potent anti-inflammatory properties.https://www.mdpi.com/1420-3049/25/8/18945-lipoxygenasemicrosomal prostaglandin E<sub>2</sub> synthasenitric oxidemedicinal plantsantimicrobialdisinfectant |
| spellingShingle | Mayuri Napagoda Jana Gerstmeier Hannah Butschek Sudhara De Soyza Simona Pace Sybille Lorenz Mallique Qader Sanjeeva Witharana Ajith Nagahawatte Gaya Wijayaratne Aleš Svatoš Lalith Jayasinghe Andreas Koeberle Oliver Werz The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka Molecules 5-lipoxygenase microsomal prostaglandin E<sub>2</sub> synthase nitric oxide medicinal plants antimicrobial disinfectant |
| title | The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka |
| title_full | The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka |
| title_fullStr | The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka |
| title_full_unstemmed | The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka |
| title_short | The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka |
| title_sort | anti inflammatory and antimicrobial potential of selected ethnomedicinal plants from sri lanka |
| topic | 5-lipoxygenase microsomal prostaglandin E<sub>2</sub> synthase nitric oxide medicinal plants antimicrobial disinfectant |
| url | https://www.mdpi.com/1420-3049/25/8/1894 |
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