Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and Neurons
The consumption of energy drinks is continuously rising, particularly in children and adolescents. While risks for adverse health effects, like arrhythmia, have been described, effects on neural cells remain elusive. Considering that neurodevelopmental processes like myelination and neuronal network...
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MDPI AG
2019-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/11/1381 |
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| author | Meray Serdar Annika Mordelt Katharina Müser Karina Kempe Ursula Felderhoff-Müser Josephine Herz Ivo Bendix |
| author_facet | Meray Serdar Annika Mordelt Katharina Müser Karina Kempe Ursula Felderhoff-Müser Josephine Herz Ivo Bendix |
| author_sort | Meray Serdar |
| collection | DOAJ |
| description | The consumption of energy drinks is continuously rising, particularly in children and adolescents. While risks for adverse health effects, like arrhythmia, have been described, effects on neural cells remain elusive. Considering that neurodevelopmental processes like myelination and neuronal network formation peak in childhood and adolescence we hypothesized that developing oligodendrocytes and neurons are particularly vulnerable to main energy drink components. Immature oligodendrocytes and hippocampal neurons were isolated from P0-P1 Wistar rats and were incubated with 0.3 mg/mL caffeine and 4 mg/mL taurine alone or in combination for 24 h. Analysis was performed immediately after treatment or after additional three days under differentiating conditions for oligodendrocytes and standard culture for neurons. Oligodendrocyte degeneration, proliferation, and differentiation were assessed via immunocytochemistry and immunoblotting. Neuronal integrity was investigated following immunocytochemistry by analysis of dendrite outgrowth and axonal morphology. Caffeine and taurine induced an increased degeneration and inhibited proliferation of immature oligodendrocytes accompanied by a decreased differentiation capacity. Moreover, dendritic branching and axonal integrity of hippocampal neurons were negatively affected by caffeine and taurine treatment. The negative impact of caffeine and taurine on developing oligodendrocytes and disturbed neuronal morphology indicates a high risk for disturbed neurodevelopment in children and adolescents by excessive energy drink consumption. |
| first_indexed | 2024-03-12T09:02:06Z |
| format | Article |
| id | doaj.art-c6b57ad74b3d4bb79f034e26977cd3b7 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T09:02:06Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-c6b57ad74b3d4bb79f034e26977cd3b72023-09-02T15:38:32ZengMDPI AGCells2073-44092019-11-01811138110.3390/cells8111381cells8111381Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and NeuronsMeray Serdar0Annika Mordelt1Katharina Müser2Karina Kempe3Ursula Felderhoff-Müser4Josephine Herz5Ivo Bendix6Department of Paediatrics I, Neonatology & Experimental perinatal Neurosciences, University Hospital Essen, University Duisburg-Essen, Essen, GermanyDepartment of Paediatrics I, Neonatology & Experimental perinatal Neurosciences, University Hospital Essen, University Duisburg-Essen, Essen, GermanyDepartment of Paediatrics I, Neonatology & Experimental perinatal Neurosciences, University Hospital Essen, University Duisburg-Essen, Essen, GermanyDepartment of Paediatrics I, Neonatology & Experimental perinatal Neurosciences, University Hospital Essen, University Duisburg-Essen, Essen, GermanyDepartment of Paediatrics I, Neonatology & Experimental perinatal Neurosciences, University Hospital Essen, University Duisburg-Essen, Essen, GermanyDepartment of Paediatrics I, Neonatology & Experimental perinatal Neurosciences, University Hospital Essen, University Duisburg-Essen, Essen, GermanyDepartment of Paediatrics I, Neonatology & Experimental perinatal Neurosciences, University Hospital Essen, University Duisburg-Essen, Essen, GermanyThe consumption of energy drinks is continuously rising, particularly in children and adolescents. While risks for adverse health effects, like arrhythmia, have been described, effects on neural cells remain elusive. Considering that neurodevelopmental processes like myelination and neuronal network formation peak in childhood and adolescence we hypothesized that developing oligodendrocytes and neurons are particularly vulnerable to main energy drink components. Immature oligodendrocytes and hippocampal neurons were isolated from P0-P1 Wistar rats and were incubated with 0.3 mg/mL caffeine and 4 mg/mL taurine alone or in combination for 24 h. Analysis was performed immediately after treatment or after additional three days under differentiating conditions for oligodendrocytes and standard culture for neurons. Oligodendrocyte degeneration, proliferation, and differentiation were assessed via immunocytochemistry and immunoblotting. Neuronal integrity was investigated following immunocytochemistry by analysis of dendrite outgrowth and axonal morphology. Caffeine and taurine induced an increased degeneration and inhibited proliferation of immature oligodendrocytes accompanied by a decreased differentiation capacity. Moreover, dendritic branching and axonal integrity of hippocampal neurons were negatively affected by caffeine and taurine treatment. The negative impact of caffeine and taurine on developing oligodendrocytes and disturbed neuronal morphology indicates a high risk for disturbed neurodevelopment in children and adolescents by excessive energy drink consumption.https://www.mdpi.com/2073-4409/8/11/1381energy drinkscaffeinetaurineneuronoligodendrocytes |
| spellingShingle | Meray Serdar Annika Mordelt Katharina Müser Karina Kempe Ursula Felderhoff-Müser Josephine Herz Ivo Bendix Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and Neurons Cells energy drinks caffeine taurine neuron oligodendrocytes |
| title | Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and Neurons |
| title_full | Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and Neurons |
| title_fullStr | Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and Neurons |
| title_full_unstemmed | Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and Neurons |
| title_short | Detrimental Impact of Energy Drink Compounds on Developing Oligodendrocytes and Neurons |
| title_sort | detrimental impact of energy drink compounds on developing oligodendrocytes and neurons |
| topic | energy drinks caffeine taurine neuron oligodendrocytes |
| url | https://www.mdpi.com/2073-4409/8/11/1381 |
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