Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain
Yaswanth Kuthati,1 Prabhakar Busa,2 Venkata Naga Goutham Davuluri,3 Chih Shung Wong1 1Department of Anesthesiology, Cathy General Hospital, Taipei, Taiwan; 2Department of Life Sciences, National Dong Hwa University, Hualien, Taiwan; 3Department of Microbiology and Immunology, College of Medicine, Na...
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Dove Medical Press
2019-12-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/manganese-oxide-nanozymes-ameliorate-mechanical-allodynia-in-a-rat-mod-peer-reviewed-article-IJN |
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| author | Kuthati Y Busa P Goutham Davuluri VN Wong CS |
| author_facet | Kuthati Y Busa P Goutham Davuluri VN Wong CS |
| author_sort | Kuthati Y |
| collection | DOAJ |
| description | Yaswanth Kuthati,1 Prabhakar Busa,2 Venkata Naga Goutham Davuluri,3 Chih Shung Wong1 1Department of Anesthesiology, Cathy General Hospital, Taipei, Taiwan; 2Department of Life Sciences, National Dong Hwa University, Hualien, Taiwan; 3Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanCorrespondence: Chih Shung WongDepartment of Anesthesiology, Cathay General Hospital, #280, Renai Road, Section 4, Taipei, TaiwanTel +886 2 27082121 Ext 3510Email w82556@gmail.comBackground: Reactive oxygen species (ROS) induced oxidative stress is linked to numerous neurological diseases, including neuropathic pain. Natural ROS scavenging enzymes like superoxide dismutase (SOD) and catalase have been found to be efficient in alleviating neuropathic pain. However, their sensitivity towards extreme pH and a short half-life limit their efficacy in vivo. Manganese oxide nanoparticles (MONPs) are recently known to possess ROS scavenging properties. In this study, MONPs were examined for their therapeutic effect on neuropathic pain.Methods: The MONPs were synthesized by a hydrothermal method. The synthesized MONPs were characterized by UV/Vis, TEM, SEM, FTIR, NTA and XRD. The biocompatibility of the nanoparticles is evaluated in neural cells using LDH assay. MONPs were evaluated for their antioxidant activity by DPPH assay. In addition, in vitro ROS scavenging properties were examined in bone marrow-derived macrophage (BMDM) cells using 2ʹ,7ʹ-dichlorofluorescin diacetate (DCFDA) assay. To evaluate the in vivo efficacy of nanoparticles, neuropathic pain was induced in Wistar rats by partial sciatic nerve transection (PSNT). On post-transection days 14 to 18, rats were intrathecally injected with MONPs and paw withdrawal threshold was measured. The spinal cords were collected and processed for Western blotting and histological analysis.Results: The synthesized MONPs were biocompatible and showed effective antioxidant activity against DPPH free radical scavenging. Further, the nanoparticles scavenged ROS efficiently in vitro in BMDM and their intrathecal administration significantly reduced mechanical allodynia as well as the expression of cyclooxygenase-2 (COX-2), an important mediator of chronic and inflammatory pain in the spinal dorsal horns of PSNT rats.Conclusion: As ROS play a significant role in neuropathic pain, we expect that MONPs could be a promising tool for the treatment of various inflammatory diseases and might also serve as a potential nanocarrier for the delivery of analgesics.Keywords: allodynia, MONPs, reactive oxygen species and neuropathic pain |
| first_indexed | 2024-12-18T04:38:51Z |
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| language | English |
| last_indexed | 2024-12-18T04:38:51Z |
| publishDate | 2019-12-01 |
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| series | International Journal of Nanomedicine |
| spelling | doaj.art-c6b85f07dada4c34be545c5f7a3146792022-12-21T21:20:47ZengDove Medical PressInternational Journal of Nanomedicine1178-20132019-12-01Volume 14101051011750743Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic PainKuthati YBusa PGoutham Davuluri VNWong CSYaswanth Kuthati,1 Prabhakar Busa,2 Venkata Naga Goutham Davuluri,3 Chih Shung Wong1 1Department of Anesthesiology, Cathy General Hospital, Taipei, Taiwan; 2Department of Life Sciences, National Dong Hwa University, Hualien, Taiwan; 3Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanCorrespondence: Chih Shung WongDepartment of Anesthesiology, Cathay General Hospital, #280, Renai Road, Section 4, Taipei, TaiwanTel +886 2 27082121 Ext 3510Email w82556@gmail.comBackground: Reactive oxygen species (ROS) induced oxidative stress is linked to numerous neurological diseases, including neuropathic pain. Natural ROS scavenging enzymes like superoxide dismutase (SOD) and catalase have been found to be efficient in alleviating neuropathic pain. However, their sensitivity towards extreme pH and a short half-life limit their efficacy in vivo. Manganese oxide nanoparticles (MONPs) are recently known to possess ROS scavenging properties. In this study, MONPs were examined for their therapeutic effect on neuropathic pain.Methods: The MONPs were synthesized by a hydrothermal method. The synthesized MONPs were characterized by UV/Vis, TEM, SEM, FTIR, NTA and XRD. The biocompatibility of the nanoparticles is evaluated in neural cells using LDH assay. MONPs were evaluated for their antioxidant activity by DPPH assay. In addition, in vitro ROS scavenging properties were examined in bone marrow-derived macrophage (BMDM) cells using 2ʹ,7ʹ-dichlorofluorescin diacetate (DCFDA) assay. To evaluate the in vivo efficacy of nanoparticles, neuropathic pain was induced in Wistar rats by partial sciatic nerve transection (PSNT). On post-transection days 14 to 18, rats were intrathecally injected with MONPs and paw withdrawal threshold was measured. The spinal cords were collected and processed for Western blotting and histological analysis.Results: The synthesized MONPs were biocompatible and showed effective antioxidant activity against DPPH free radical scavenging. Further, the nanoparticles scavenged ROS efficiently in vitro in BMDM and their intrathecal administration significantly reduced mechanical allodynia as well as the expression of cyclooxygenase-2 (COX-2), an important mediator of chronic and inflammatory pain in the spinal dorsal horns of PSNT rats.Conclusion: As ROS play a significant role in neuropathic pain, we expect that MONPs could be a promising tool for the treatment of various inflammatory diseases and might also serve as a potential nanocarrier for the delivery of analgesics.Keywords: allodynia, MONPs, reactive oxygen species and neuropathic painhttps://www.dovepress.com/manganese-oxide-nanozymes-ameliorate-mechanical-allodynia-in-a-rat-mod-peer-reviewed-article-IJNallodyniamonpsreactive oxygen species and neuropathic pain |
| spellingShingle | Kuthati Y Busa P Goutham Davuluri VN Wong CS Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain International Journal of Nanomedicine allodynia monps reactive oxygen species and neuropathic pain |
| title | Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain |
| title_full | Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain |
| title_fullStr | Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain |
| title_full_unstemmed | Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain |
| title_short | Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain |
| title_sort | manganese oxide nanozymes ameliorate mechanical allodynia in a rat model of partial sciatic nerve transection induced neuropathic pain |
| topic | allodynia monps reactive oxygen species and neuropathic pain |
| url | https://www.dovepress.com/manganese-oxide-nanozymes-ameliorate-mechanical-allodynia-in-a-rat-mod-peer-reviewed-article-IJN |
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