Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancer
Breast cancer is a major health threat to women globally. Many circulating microRNAs are non-invasive cancer biomarkers. In this study, the expression of miR-29b and miR-31 was assessed in blood samples from 200 patients with breast cancer and wholesome volunteer women using quantitative reverse tra...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2022-12-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054022000488 |
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| author | Mona Ahmed Abbas Ibrahim El Tantawy El Sayed Azza Mohamed Kamel Abdu-Allah Abul Kalam Abdullah G. Al-Sehemi Omar A. Al-Hartomy Maha Salah Abd El-rahman |
| author_facet | Mona Ahmed Abbas Ibrahim El Tantawy El Sayed Azza Mohamed Kamel Abdu-Allah Abul Kalam Abdullah G. Al-Sehemi Omar A. Al-Hartomy Maha Salah Abd El-rahman |
| author_sort | Mona Ahmed Abbas |
| collection | DOAJ |
| description | Breast cancer is a major health threat to women globally. Many circulating microRNAs are non-invasive cancer biomarkers. In this study, the expression of miR-29b and miR-31 was assessed in blood samples from 200 patients with breast cancer and wholesome volunteer women using quantitative reverse transcriptase PCR to evaluate their role in the disease. MiR-29b was significantly overexpressed in patients compared to controls. Multivariate regression analysis showed that it was an established risk factor for relapse and mortality. MiR-31 was significantly under-expressed in patients. It was an established risk factor for relapse and was strongly associated with mortality. For the prediction of relapse, miR-29b had a sensitivity of 81.25% and a specificity of 88.24% at a cutoff of > 30.09, while miR-31 had a sensitivity of 87.50% and a specificity of 79.41% at a cutoff of 0.12. The specificity was enhanced to 100% by combining the values of miR-29b and miR-31. In predicting mortality, miR-29b exhibited a sensitivity of 90% and a specificity of 97.5% at a cutoff of > 48.10. At a cutoff of 0.119, miR-31 exhibited a sensitivity of 87.50% and a specificity of 79.41%. High miR-29b expression and low miR-31 expression were linked with a low survival rate. MiR-29b and miR-31 could be useful markers for predicting breast cancer relapse and mortality. |
| first_indexed | 2024-04-11T18:51:04Z |
| format | Article |
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| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-04-24T08:42:33Z |
| publishDate | 2022-12-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Non-coding RNA Research |
| spelling | doaj.art-c6b863693301486a9974b0698544f2552024-04-16T15:16:24ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402022-12-0174248257Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancerMona Ahmed Abbas0Ibrahim El Tantawy El Sayed1Azza Mohamed Kamel Abdu-Allah2Abul Kalam3Abdullah G. Al-Sehemi4Omar A. Al-Hartomy5Maha Salah Abd El-rahman6Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menoufia, EgyptDepartment of Chemistry, Faculty of Science, Menoufia University, Egypt; Corresponding author.Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menoufia, Egypt; Medical Laboratory Department, College of Applied Medical Sciences, Taibah University, Yanbu, Saudi ArabiaResearch Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia; Department of Chemistry, College of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi ArabiaResearch Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia; Department of Chemistry, College of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi ArabiaDepartment of Physics, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaDepartment of Chemistry, Faculty of Science, Menoufia University, EgyptBreast cancer is a major health threat to women globally. Many circulating microRNAs are non-invasive cancer biomarkers. In this study, the expression of miR-29b and miR-31 was assessed in blood samples from 200 patients with breast cancer and wholesome volunteer women using quantitative reverse transcriptase PCR to evaluate their role in the disease. MiR-29b was significantly overexpressed in patients compared to controls. Multivariate regression analysis showed that it was an established risk factor for relapse and mortality. MiR-31 was significantly under-expressed in patients. It was an established risk factor for relapse and was strongly associated with mortality. For the prediction of relapse, miR-29b had a sensitivity of 81.25% and a specificity of 88.24% at a cutoff of > 30.09, while miR-31 had a sensitivity of 87.50% and a specificity of 79.41% at a cutoff of 0.12. The specificity was enhanced to 100% by combining the values of miR-29b and miR-31. In predicting mortality, miR-29b exhibited a sensitivity of 90% and a specificity of 97.5% at a cutoff of > 48.10. At a cutoff of 0.119, miR-31 exhibited a sensitivity of 87.50% and a specificity of 79.41%. High miR-29b expression and low miR-31 expression were linked with a low survival rate. MiR-29b and miR-31 could be useful markers for predicting breast cancer relapse and mortality.http://www.sciencedirect.com/science/article/pii/S2468054022000488microRNABreast cancerBiomarkerqRT-PCRExpression |
| spellingShingle | Mona Ahmed Abbas Ibrahim El Tantawy El Sayed Azza Mohamed Kamel Abdu-Allah Abul Kalam Abdullah G. Al-Sehemi Omar A. Al-Hartomy Maha Salah Abd El-rahman Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancer Non-coding RNA Research microRNA Breast cancer Biomarker qRT-PCR Expression |
| title | Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancer |
| title_full | Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancer |
| title_fullStr | Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancer |
| title_full_unstemmed | Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancer |
| title_short | Expression of MiRNA-29b and MiRNA-31 and their diagnostic and prognostic values in Egyptian females with breast cancer |
| title_sort | expression of mirna 29b and mirna 31 and their diagnostic and prognostic values in egyptian females with breast cancer |
| topic | microRNA Breast cancer Biomarker qRT-PCR Expression |
| url | http://www.sciencedirect.com/science/article/pii/S2468054022000488 |
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